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Immunotherapeutic Effects of Glycyrrhiza Glabra and Glycyrrhizic Acid on Leishmania Major-Infection Balb/C Mice Publisher Pubmed



Sheikhi S1 ; Khamesipour A2 ; Radjabian T3 ; Ghazanfari T4 ; Miramin Mohammadi A2
Authors
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Authors Affiliations
  1. 1. Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran
  2. 2. Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
  4. 4. Immunoregulation Research Center, Shahed University, Tehran, Iran

Source: Parasite Immunology Published:2022


Abstract

Treatment of cutaneous leishmaniasis (CL) is a public health problem in endemic areas. The objective of the current study was to investigate the immunotherapeutic activities of the hydroalcoholic extract of Glycyrrhiza glabra (HEG) and glycyrrhizic acid (GA) in the treatment of Leishmania major (L. major)-infected BALB/c mice. In this study, the effect of HEG and GA was checked in vitro on growth of L. major promastigote and amastigote using MTT assay and microscopic counting, respectively. For in vivo experiment, the lesion induced by L. major on BALB/c mice were treated intraperitoneally with HEG, GA, meglumine antimoniate or phosphate buffer saline (negative control) for one month. Then, the lesion development and the parasite burden of the lymph node was assessed, the cytokine response (IFN-γ and IL-4) to Leishmania antigens was evaluated using ELISA method. The results showed that HEG and GA significantly inhibited the growth of L. major promastigotes and amastigotes, the lesion development, parasite burden in the lymph nodes, level of IFN-γ and the ratio of IFN-γ/IL-4 in HEG, GA and meglumine antimoniate-treated mice were significantly higher compared with the negative control group, there was no difference between the HEG, GA and meglumine antimoniate group. It is concluded that hydroalcoholic extract of G. glabra and glycyrrhizic acid showed therapeutic and immunomodulatory effects on L. major-infected BALB/c mice. © 2021 John Wiley & Sons Ltd
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