Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Association of Mir-451 and Mir-21 in Plasma With Lymph Node Metastases in Breast Cancer



Motamedi M1 ; Hashemzadeh Chaleshtori M1 ; Ghasemi S1 ; Kheiri S2 ; Haji Gholami A3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Cellular and Molecular Biology Research Center, Basic Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  2. 2. Department of Biostatistics and Epidemiology, Faculty of Public Health, Shahrekord University of Medical Sciences, Shahrekord, Iran
  3. 3. Faculty of Medicine, Isfahan University of Medical Sciences, Iran

Source: Journal of Babol University of Medical Sciences Published:2018

Abstract

BACKGROUND AND OBJECTIVE: The expression of some circulating microRNAs (miRNAs) in biological fluids of healthy individuals is different from cancerous patients. circulating miRNAs are a new class of cancer biomarkers because of their high stability and sensitivity, ease of measurement and specificity due to their correlation with various cancer states. According to the miR-451 and miR-21 functions in the metastasis of some cancers, the aim of this study was to investigate the differences of expression levels of miR-451 and miR-21 in the plasma of breast cancer (BC) patients with and without lymph nodes metastasis (LNM). METHODS: In this descriptive-analytical study, blood samples were collected from 47 women with BC and 24 healthy women with mammography confirmation. The presence/or absence of LNM was recognized from patients' medical records. The expression levels of miR-451 and miR-21 in the plasma, were investigated using Real-Time PCR. FINDINGS: The median of expression of miR-451 in BC patients with LNM and without LNM was 1.739 and 3.187, respectively, and its expression in lymph node metastatic patients decreased 0.444 folds in comparison with non-metastatic patients (p=0.031). The median of expression of miR-21 in patients with LNM and in non-metastatic lymph nodes patients was 5.922 and 2.157, respectively, and its expression in metastatic status was 2 folds higher than non-metastatic (p=0.029). CONCLUSION: The results of this study indicated that decreased miR-451 and increased miR-21 expression in plasma of BC patients was associated with LNM status. © 2018, Babol University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Plasma Level of Mir-21 and Mir-451 in Primary and Recurrent Breast Cancer Patients, Breast Cancer: Targets and Therapy (2019)
2. Mir-370 Expression Analysis in Breast Cancer, Journal of Isfahan Medical School (2015)
3. Differential Expression Profile of Mir-27B, Mir-29A, and Mir-155 in Chronic Lymphocytic Leukemia and Breast Cancer Patients, Molecular Therapy Oncolytics (2020)
4. Exploring Plasma Expression Levels of Mir-200C-3P, Mir-150, and Mir-155 in Sars-Cov-2 Positive and Negative Subjects, Future Virology (2024)
5. Evaluation of Mir-146A As a Potential Biomarker for Diagnosis of Cardiotoxicity Induced by Chemotherapy in Patients With Breast Cancer, Journal of Research in Medical Sciences (2025)
6. Emerging Circulating Mirnas and Lncrnas in Upper Gastrointestinal Cancers, Expert Review of Molecular Diagnostics (2020)
7. Circulating Lncrna Hotair Is a Biomarker for Pediatric Acute Lymphoblastic Leukemia and Mediator of Mir-326 Exosomal Export, Scientific Reports (2025)
8. Circulating Micrornas As Potential Diagnostic Biomarkers and Therapeutic Targets in Gastric Cancer: Current Status and Future Perspectives, Current Medicinal Chemistry (2016)
Experts (# of related papers)
View all Related Experts
Mohammadreza Sharifi (8)
Rasoul Salehi (6)
Other Related Docs
9. Melatonin Affects the Expression of Microrna-21: A Mini-Review of Current Evidence, Pathology Research and Practice (2024)
10. Emerging Role of Mir-372 and Mir-101A in Head and Neck Squamous Cell Carcinoma, Clinical Laboratory (2020)
11. A Rapid and Simple Method for Simultaneous Determination of Three Breast Cancer Related Micrornas Based on Magnetic Nanoparticles Modified With S9.6 Antibody, Analytical Biochemistry (2023)
12. Bioinformatics Prediction and Experimental Validation of a Novel Microrna: Hsa-Mir-B43 Within Human Cdh4 Gene With a Potential Metastasis-Related Function in Breast Cancer, Journal of Cellular Biochemistry (2020)
13. Mir-195 Down-Regulation Is a Distinctive Biomarker of Her2 Positive State in Breast Cancer, Gene Reports (2020)
14. Circulating Micrornas in Hepatocellular Carcinoma: Potential Diagnostic and Prognostic Biomarkers, Current Pharmaceutical Design (2016)
15. Evaluation of Microrna Expression Pattern (Mir-28, Mir-181A, Mir-34A, and Mir-31) in Patients With Covid-19 Admitted to Icu and Diabetic Covid-19 Patients, Intervirology (2023)
16. Apoptosis-Inducing and Antiproliferative Effect by Inhibition of Mir-182-5P Through the Regulation of Casp9 Expression in Human Breast Cancer, Cancer Gene Therapy (2017)
17. Evaluation of the Expressed Mir‑129 and Mir‑549A in Patients With Multiple Sclerosis, Advanced Biomedical Research (2021)
18. Locked Nucleic Acid Anti-Mir-21 Inhibits Cell Growth and Invasive Behaviors of a Colorectal Adenocarcinoma Cell Line: Lna-Anti-Mir As a Novel Approach, Cancer Gene Therapy (2016)
19. Pbmcs: A New Source of Diagnostic and Prognostic Biomarkers, Archives of Physiology and Biochemistry (2022)
20. Current Status and Perspectives Regarding Lna-Anti-Mir Oligonucleotides and Microrna Mir-21 Inhibitors As a Potential Therapeutic Option in Treatment of Colorectal Cancer, Journal of Cellular Biochemistry (2017)
21. The Importance of Snps at Mirna Binding Sites As Biomarkers of Gastric and Colorectal Cancers: A Systematic Review, Journal of Personalized Medicine (2022)
22. Breast Cancer Diagnosis: Imaging Techniques and Biochemical Markers, Journal of Cellular Physiology (2018)
23. Emerging Biologic and Clinical Implications of Mir-182-5P in Gynecologic Cancers, Clinical and Translational Oncology (2024)
24. Identification of Potential Biomarkers in the Peripheral Blood Mononuclear Cells of Relapsing–Remitting Multiple Sclerosis Patients, Inflammation (2022)
25. Microrna-200C Overexpression in Cancer-Associated Fibroblasts Reduces the Invasive Properties of Breast Tumour Cells, Clinical and Translational Discovery (2022)
26. Microrna Profiles in Critically Ill Patients, Current Medicinal Chemistry (2024)
27. A Meta-Analysis of Microrna Expression Profiling Studies in Heart Failure, Heart Failure Reviews (2021)
28. Candidate Microrna Biomarkers in Lupus Nephritis: A Meta-Analysis of Profiling Studies in Kidney, Blood and Urine Samples, Molecular Diagnosis and Therapy (2023)
29. Comparison of Mir-211 Expression in Murine Melanoma Cell Line and Murine Melanoma Tumor by Real-Time Polymerase Chain Reaction, Journal of Isfahan Medical School (2012)
30. Deciphering the Multifaceted Role of Micrornas in Hepatocellular Carcinoma: Integrating Literature Review and Bioinformatics Analysis for Therapeutic Insights, Heliyon (2024)
31. Therapeutic Inhibition of Microrna-21 (Mir-21) Using Locked-Nucleic Acid (Lna)-Anti-Mir and Its Effects on the Biological Behaviors of Melanoma Cancer Cells in Preclinical Studies, Cancer Cell International (2020)
32. The Role of Mir-16 and Mir-34A Family in the Regulation of Cancers: A Review, Heliyon (2025)
33. Mir-574, Mir-499, Mir-125B, Mir-106A, and Mir-9 Potentially Target Tgfbr-1 and Tgfbr-2 Genes Involving in Inflammatory Response Pathway: Potential Novel Biomarkers for Chronic Lymphocytic Leukemia, Pathology Research and Practice (2022)
34. In Silico Identification and in Vitro Evaluation of Mrps30-Dt Lncrna and Mrps30 Gene Expression in Breast Cancer, Cancer Reports (2024)
35. Micrornas Regulating Wnt Signaling Pathway in Colorectal Cancer: Biological Implications and Clinical Potentials, Functional and Integrative Genomics (2022)
36. The Expression of Ck-19 Gene in Circulating Tumor Cells of Blood Samples of Metastatic Breast Cancer Women, Research in Pharmaceutical Sciences (2015)
37. A Review on Expression and Regulatory Mechanisms of Mir-337-3P in Cancer, Journal of Biomolecular Structure and Dynamics (2024)
38. The Potential for Circulating Micrornas in the Diagnosis of Myocardial Infarction: A Novel Approach to Disease Diagnosis and Treatment, Current Pharmaceutical Design (2016)
39. Higher Plasma Mmp-9 Level in Breast Cancer Patients With Mmp-9 Promoter T Allele, Journal of Biological Research (2010)
40. Mir-939, As an Important Regulator in Various Cancers Pathogenesis, Has Diagnostic, Prognostic, and Therapeutic Values: A Review, Journal of the Egyptian National Cancer Institute (2024)
41. Investigation of Microrna-10B Values for the Discrimination of Metastasis Due to Melanoma, Journal of Research in Medical Sciences (2021)
42. Serum Mmp-9 Level Associated With Initiation and Progression Steps of Breast Cancer in the Iranian Population, Reproductive Sciences (2010)
43. Association of Serum Microrna-21 Levels With Visfatin, Inflammation, and Acute Coronary Syndromes, Heart and Vessels (2017)
44. Micrornas and Target Molecules in Bladder Cancer, Medical Oncology (2020)
45. The Upregulation of Hsa-Mir-181B-1 and Downregulation of Its Target Cyld in the Late-Stage of Tumor Progression of Breast Cancer, Indian Journal of Clinical Biochemistry (2020)
46. A H. Pylori-Independent Mir-21 Overexpression in Gastric Cancer Patients, Gene Reports (2019)
47. Mir-326 and Mir-26A, Two Potential Markers for Diagnosis of Relapse and Remission Phases in Patient With Relapsing-Remitting Multiple Sclerosis, Gene (2014)
48. Circulating Microrna-192 As a Diagnostic Biomarker in Human Chronic Lymphocytic Leukemia, Cancer Gene Therapy (2016)
49. Molecular Mechanisms of Mir-1236 in the Assessment of Tumor Lymphangiogenesis in Human Ovarian Cancer Patients, Journal of Gene Medicine (2023)
50. Evaluation of Expression Level of Mir-3135B-5P in Blood Samples of Breast Cancer Patients Experiencing Chemotherapy-Induced Cardiotoxicity, Indian Journal of Clinical Biochemistry (2023)