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Emergence of Carbapenem Resistant Escherichia Coli Isolates Producing Blandm and Blaoxa-48-Like Carried on Inca/C and Incl/M Plasmids at Two Iranian University Hospitals Publisher Pubmed



Solgi H1 ; Giske CG2 ; Badmasti F1 ; Aghamohammad S1 ; Havaei SA3 ; Sabeti S4 ; Mostafavizadeh K5 ; Shahcheraghi F1
Authors
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Authors Affiliations
  1. 1. Department of Bacteriology, Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran
  2. 2. Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute and Karolinska University Hospital, Stockholm, Sweden
  3. 3. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Pathology, Loghman Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Infection, Genetics and Evolution Published:2017


Abstract

The emergence of carbapenem resistance among Escherichia coli is a serious threat to public health. The objective of this study was to investigate resistance genes and clonality of carbapenem resistant E. coli in Iran. Between February 2015 and July 2016, a total of 32 non-duplicate E. coli isolates that were ertapenem resistant or intermediate (R/I-ETP) were collected from patient clinical or surveillance cultures (rectal swabs) at two university hospitals. Resistance genes were identified by PCR and sequencing. Conjugation experiments, PCR-based replicon typing, PFGE and multilocus sequence typing (MLST) were performed. PCR assays showed, among the 32 isolates, twenty-nine strains produced carbapenemase genes. The predominant carbapenemase was blaOXA-48 (82.8%), followed by blaNDM-1 (31%), blaNDM-7 (6.9%) and blaOXA-181 (3.4%). Seven of the blaNDM positive isolates co-harbored blaOXA-48 carbapenemases. The blaNDM and blaOXA-48 were found in IncA/C and IncL/M conjugative plasmids, respectively. The blaCTX-M-15, qnrA and intI1 genes were also present in most isolates. The PFGE revealed genetic diversity among the 28 E. coli isolates, which belonged to six minor PFGE clusters and 14 isolates were singletons. The 26 isolates were distributed into 18 STs, of which two were dominant (ST648 and ST167). We identified one blaNDM-1-positive ST131 E. coli isolates that harbor the blaCTX-M-15 and blaTEM genes. Horizontal transfer of IncA/C and IncL/M plasmids has likely facilitated the spread of the blaOXA-48 and blaNDM genes among E. coli. Their clonal diversity and the presence of faecal carriers in isolates suggest an endemic spread of OXA-48 and NDM. Therefore, it emphasizes the critical importance of monitoring and controlling the spread of carbapenem resistant E. coli. © 2017
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