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Considerable Rate of Putative Virulent Phylo-Groups in Fecal Carriage of Extended-Spectrum Β-Lactamase Producing Escherichia Coli Publisher Pubmed



Aghamohammad S1 ; Badmasti F1 ; Shirazi AS1 ; Dabiri H2 ; Solgi H3 ; Sabeti S4 ; Shahcheraghi F1
Authors
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Authors Affiliations
  1. 1. Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
  2. 2. Department of Medical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Division of Clinical Microbiology, Department of Laboratory Medicine, Amin Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Infection, Genetics and Evolution Published:2019


Abstract

Extended-Spectrum Beta–lactamase producing Enterobacteriales (ESBL-PE)in fecal carriage have become a global health concern. Detection of putative virulent ESBL-producing E.coli (ESBL-EC)isolates among asymptomatic carriers is a threatening issue in public health. The aim of this study was to investigate the intestinal carriage of ESBL-EC, phylo-groups and clonal relatedness among putative virulent groups of ESBL-EC isolated from fecal carriages. A total of 120 rectal swabs; 50.8% (61/120)from inpatients of intensive care unit (ICU)and 49.2% (59/120)from outpatients were collected. The ESBL-EC screening was performed by using MacConkey agar supplemented with cefotaxime. PCR assays were applied for determination of phylo-groups, detection of ESBL and carbapenemase genes. Conjugation experiment, plasmid replicon typing and Multilocus Sequence Typing (MLST)were performed for putative virulent phylo-groups. Totally, of 120 studied individuals, 60.0% (72/120)were carrier for ESBL-EC. The rate of blaCTX-M-15, blaTEM, blaSHV was 90.2% (65/72), 50.0% (36/72)and 5.5% (4/72), respectively. The frequency of phylo-groups A, B1, B2, C, D, and F were 20.8% (15/72), 6.9% (5/72), 20.8% (15/72), 2.7% (2/72), 13.8 (10/72)and 12.5% (9/72), respectively. In conjugation experiments, of 6 tested isolates, 5 had conjugative plasmids. The most prevalent plasmid types belonged to IncF incompatibility groups. The MLST analysis showed that the main sequence types among ESBL-EC isolates were ST769 and ST472. The current study provides novel information about the presence of the ESBL-EC isolates, particularly putative virulent phylo-groups among fecal carriages in Iran. Our data revealed that there was almost high ST heterogeneity among putative ESBL-EC isolates. In order to implementation of effective infection control program, detection of fecal carriage in appropriate time typically at the beginning of admission to the hospital is recommended. © 2019 Elsevier B.V.
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