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Evaluation of Prevalence of New Candida Species (C. Orthopsilosis and C. Metapsilosis) Among C. Parapsilosis Group Isolated From Patients by Pcr-Rflp of Sadh Gene in Iran



Mohammadi R1 ; Mirhendi H2 ; Yadegari MH1 ; Ghahri M3 ; Shadzi S4 ; Jalalizand N5 ; Hedayati MT6
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Authors Affiliations
  1. 1. Department of Medical Mycology, Tarbiat Modares University, Tehran, Iran
  2. 2. Department of Medical Mycology and Parasitology, School of Health and Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Mycology, School of Applied Sciences, Imam Hossein University, Tehran, Iran
  4. 4. Department of Medical Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Source: Journal of Isfahan Medical School Published:2011

Abstract

Background: Candida orthopsilosis and C. metapsilosis are recently introduced species that phenotipically indistinguishable from C. parapsilosis. In the present study, we evaluated the incidence and distribution of C. orthopsilosis and C. metapsilosis among 111 C. parapsilosis, isolated from different body locations by molecular methods. Methods: Yeast genomic DNAs were extracted from 935 isolates using Whatman FTA filter matrix technology from fresh cultures. First, ITS region of yeasts, were amplified by ITS1 and ITS4 primers, then Candida parapsilosis group were identified with digestion by MspI. Then SADH gene was amplified and three species of candida including C. parapsilosis, C. orthopsilosis and C. metapsilosis were distincted with digestion by NlaIII enzyme. Finding: Totally 111 isolates were identified as Candida parapsilosis that among them, 18 isolates (16.2%) were identified as C. orthopsilosis and 93 isolates (83.7%) as C. parapsilosis. No isolates were identified as C. metapsilosis. The most isolates of C. orthopsilosis, were obtained from nail. Conclusion: C. ortopsilosis is not a rare species in Iran and it seems that a significant amounts of Candida strains which are considered as C. parapsilosis might be C. ortopsilosis.
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