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Evaluation of the Frequency of Osteopontin Gene Polymorphism (Rs28357094 T>G) and Its Receptor Gene (Itga4- Rs1449263 A>G) in Multiple Sclerosis Patients in Comparison to Healthy Controls in Isfahan, Iran, Population



Dadkhah F1 ; Alsahebfosoul F2 ; Eskandari N2 ; Salehi M3 ; Etemadifar M4 ; Kazemi M4
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Medicine AND Student Research Committee, Isfahan University of Medical Science, Isfahan, Iran
  2. 2. Department of Immunology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
  3. 3. Department of Cellular and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
  4. 4. Department of Neurosciences, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
  5. 5. Iran

Source: Journal of Isfahan Medical School Published:2016

Abstract

Background: Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS). Various factors including genetic and environmental factors are involved in the prevalence of MS. The Osteopontin (OPN) and its receptor Integrins alpha-4/beta-1 (ITGA4) have important role in immune response, so their encoding gene could be considered as a candidate for susceptibility to MS diseases. In the present study, we evaluated the frequency of OPN (rs28357094T > G) and ITGA4 (rs1449263A>G) polymorphisms in MS patients compared with healthy controls in Isfahan, Iran population. Methods: Blood samples were collected from 100 patients with multiple sclerosis and 100 healthy controls. After DNA extraction, OPN (rs28357094) and ITGA4(rs1449263) polymorphisms were detected with high resolution melt real time polymerase chain reaction (HRM Real Time PCR) technique. The results were analyzed with the SPSS software. Findings: In this study, frequency of TT genotype (OPN-rs28357094) (P = 0.020, OR = 1.85) and AA genotype (ITGA4 - rs1449263) (P = 0.012, OR = 2.10) in Patients with multiple sclerosis were higher than the control group significantly. Conclusion: The results of this study show that rs28357094 T>G on OPN gene and ITGA4-rs1449263A > G polymorphisms were associated with susceptibility to multiple sclerosis in Isfahan population. © 2016, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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