Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Apoptosis-Inducing Effect of Spiroaminopyrimidine Analogue in Nb4 Leukemia Cells Via Down-Regulation of Birc5 Expression Publisher Pubmed



Dehghan Nayeri MJ1 ; Mahdavi M1 ; Fazeli H2 ; Hosseinpour Feizi MA1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, 5166616471, Iran
  2. 2. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 817467346, Iran

Source: Experimental Oncology Published:2019


Abstract

Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP) on NB4 acute promyelocytic leukemia (APL) cells. Materials and Methods: The cells were treated with 10-100 µM of 9-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst 33258 staining as well as by Annexin V/PI double staining. Results: The compound under study was found to be highly active cell growth inhibitor with IC50 of 30.0 ± 3.5 µM inducing apoptosis in NB4 cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G1 cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC5 expression in a time-dependent manner. Conclusion: 9-tBAP displayed potent anti-leukemic activity in vitro thus warranting further investigation. © Experimental Oncology, 2019
Related Docs
View other Related Docs
1. Formononetin and Dihydroartemisinin Act Synergistically to Induce Apoptosis in Human Acute Myeloid Leukemia Cell Lines, Cell Journal (2024)
2. A Comparison of Thiazolyl Blue (Mtt) Versus Sulforhodamine B (Srb) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, Ags, and Pc3 Cancer Cell Lines, Journal of Isfahan Medical School (2017)
3. Novel 9-(Alkylthio)-Acenaphtho[1,2-E]-1,2,4-Triazine Derivatives: Synthesis, Cytotoxic Activity and Molecular Docking Studies on B-Cell Lymphoma 2 (Bcl-2), DARU, Journal of Pharmaceutical Sciences (2014)
4. Synthesis and Biological Evaluation of Bidentate 3-Hydroxypyridin-4-Ones Iron Chelating Agents, Research in Pharmaceutical Sciences (2011)
5. Dihydroartemisinin Enhances the Therapeutic Efficacy of Bh3 Mimetic Inhibitor in Acute Lymphoblastic Leukemia Cells Via Inhibition of Mcl-1, Asian Pacific Journal of Cancer Prevention (2024)
6. Persicoimidate Isolated From Allium Ampeloprasum Subsp. Persicum With Apoptotic Effects Against Breast Cancer Cell Lines, Iranian Journal of Pharmaceutical Sciences (2020)
7. Evaluation of the Radiosensitizing Potency of Bromelain for Radiation Therapy of 4T1 Breast Cancer Cells, Journal of Medical Signals and Sensors (2019)
8. The Effects of Abt-199 and Dihydroartemisinin Combination on Cell Growth and Apoptosis in Human U937 and Kg-1 Cancer Cells, Asian Pacific Journal of Cancer Prevention (2024)
Experts (# of related papers)
View all Related Experts
Mahmoud Aghaei (8)
Hojjat Sadeghi-Aliabadi (7)
Other Related Docs
9. Cytotoxic Activities of Euphorbia Kopetdaghi Against Ovcar-3 and Ej-138 Cell Lines, Journal of HerbMed Pharmacology (2015)
10. Synthesis, Anti-Hiv-1 and Antiproliferative Evaluation of Novel 4-Nitroimidazole Derivatives Combined With 5-Hydroxy-4-Pyridinone Moiety, Journal of Molecular Structure (2020)
11. Evaluation of Cytotoxic Effects of a Dinuclearpalladacycle Derivative, Biphosphinic Complex, on Cisplatin-Resistant Ht-29 Cells, Journal of Reports in Pharmaceutical Sciences (2017)
12. Hybrid Benzoxazole-Coumarin Compounds Induce Death Receptor-Mediated Switchable Apoptotic and Necroptotic Cell Death on Hn-5 Head and Neck Cancer Cell Line, Anti-Cancer Agents in Medicinal Chemistry (2016)
13. Synthesis and Cytotoxic Evaluation of Some New 3-(2-(2-Phenylthiazol-4-Yl) Ethyl)-Quinazolin-4(3H) One Derivatives With Potential Anticancer Effects, Research in Pharmaceutical Sciences (2017)
14. Synthesis and Cytotoxic Evaluation of Two Novel Anthraquinone Derivatives, Farmaco (2004)
15. Synthesis and Cytotoxic Evaluation of Some Quinazolinone- 5-(4-Chlorophenyl) 1, 3, 4-Oxadiazole Conjugates, Research in Pharmaceutical Sciences (2019)
16. Effect of Thymoquinone on P53 Gene Expression and Consequence Apoptosis in Breast Cancer Cell Line, International Journal of Preventive Medicine (2016)
17. Monastrol Derivatives: In Silico and in Vitro Cytotoxicity Assessments, Research in Pharmaceutical Sciences (2020)
18. Synthesis and Cytotoxic Evaluation of Some Novel 3-[2-(2-Phenyl-Thiazol-4-Yl)-Ethyl] -3H-Pyrido [2,3-D]Pyrimidin-4-One Derivatives, Research in Pharmaceutical Sciences (2021)
19. Kopetdaghinanes, Pro-Apoptotic Hemiacetialic Cyclomyrsinanes From Euphorbia Kopetdaghi, Fitoterapia (2020)
20. Design, Synthesis, in Silico Studies, and Antiproliferative Evaluations of Novel Indolin-2-One Derivatives Containing 3-Hydroxy-4-Pyridinone Fragment, Bioorganic and Medicinal Chemistry Letters (2022)
21. Synthesis and Cytotoxic Evaluation of Some New 4(3H)-Quinazolinones on Hela Cell Line, Research in Pharmaceutical Sciences (2012)
22. Novel Palladium Complex: Cytotoxicity Against Cisplatin-Resistant K562 Cells, Iranian Journal of Pharmaceutical Research (2019)
23. The Art of Design in Azlactone-Benzoxazinone Chemistry, Docking Studies and in Vitro Cytotoxicity Evaluation, Australian Journal of Chemistry (2022)
24. Britannin, a Sesquiterpene Lactone, Inhibits Proliferation and Induces Apoptosis Through the Mitochondrial Signaling Pathway in Human Breast Cancer Cells, Tumor Biology (2015)
25. Phytochemical Analysis of Daphne Pontica L. Stems With Their Pro-Apoptotic Properties Against Du-145 and Lncap Prostate Cancer Cells, DARU, Journal of Pharmaceutical Sciences (2022)
26. New 6(17)-Epoxylathyrane Diterpene: Aellinane From Euphorbia Aellenii Induces Apoptosis Via Mitochondrial Pathway in Ovarian Cancer Cell Line, Toxicology Mechanisms and Methods (2017)
27. All-Trans Retinoic Acid and Genistein Induce Cell Apoptosis in Ovcar-3 Cells by Increasing the P14 Tumor Suppressor Gene, Research in Pharmaceutical Sciences (2016)
28. Anticancer Activity of Delphinium Semibarbatum Alkaloid Fractions Against Lncap, and Du 145 Human Prostate Cancer Cells Through the Intrinsic Apoptotic Pathway, Iranian Journal of Pharmaceutical Research (2021)
29. Molecular Mechanism of Apoptosis Induction by Gaillardin, a Sesquiterpene Lactone, in Breast Cancer Cell Lines: Gaillardin-Induced Apoptosis in Breast Cancer Cell Lines, Cell Biology and Toxicology (2015)
30. Synthesis, Biological and Docking Studies of Thiadiazole Amide Derivatives Containing Anthranilic Acid, Journal of Sciences, Islamic Republic of Iran (2023)
31. Centaurea Cyanus Extracted 13-O-Acetylsolstitialin a Decrease Bax/Bcl-2 Ratio and Expression of Cyclin D1/Cdk-4 to Induce Apoptosis and Cell Cycle Arrest in Mcf-7 and Mda-Mb-231 Breast Cancer Cell Lines, Journal of Cellular Biochemistry (2019)
32. Protective Effect of Amifostine on Busulfan Induced Dna Damage in Human Hepatoma Cells, Toxicology Mechanisms and Methods (2017)
33. Evaluation of the Cytotoxic Effect of Hydroxypyridinone Derivatives on Hct116 and Sw480 Colon Cancer Cell Lines, Pharmaceutical Chemistry Journal (2019)
34. Evaluation of Protective Effect of Amifostine on Dacarbazine Induced Genotoxicity, Research in Pharmaceutical Sciences (2015)