Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Anticancer Activity of Delphinium Semibarbatum Alkaloid Fractions Against Lncap, and Du 145 Human Prostate Cancer Cells Through the Intrinsic Apoptotic Pathway Publisher



Lotfaliani M1, 2 ; Ghanadian M3 ; Ayatollahi SA2, 4 ; Aghaei M5 ; Kobarfard F6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Science, Tehran, Iran
  2. 2. Department of Pharmacognosy, School of Pharmacy, Shaheed Sadoughi University of Medical Science and Health Service, Yazd, Iran
  3. 3. Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Phytochemistry Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran
  5. 5. Department of Clinical Biochemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Medical Chemistry, Phytochemistry Research Center, School of Pharmacy, Shahid Beheshti University of Medical Science, Tehran, Iran

Source: Iranian Journal of Pharmaceutical Research Published:2021


Abstract

Prostate cancer is one of the common cancers with a high mortality rate in men. Therefore, there is always a necessity to discover new medications for treatment or alleviating its symptoms. In recent years, anticancer properties of a number of delphinium species were reported, but there is no study on the anticancer effects of Delphinium semibarbatum (D. semibarbatum) alkaloid contents. Therefore, this survey aimed to check the cytotoxicity and apoptotic properties of D. semibarbatum alkaloid fractions (DSAFs) against prostate cancer cells. Cytotoxicity was measured by MTT assay. We examined the apoptosis by detecting annexin V-FITC/PI staining, the mitochondrial membrane potential (ΔΨm) disruption, reactive oxygen species (ROS) generation, the activity of caspase-3, and expression of the Bax and Bcl-2 in cancer cells. DSAFs treatment inhibited the growth of LNCaP and DU‐145 cells by the increase of apoptotic (Q2+Q3) cells detected by annexin V/PI assay. We found over-generation of intracellular ROS and ΔΨm loss in mitochondrial membrane potential treated cell lines. Attenuation of anti-apoptotic Bcl-2 followed by the increase in pro-apoptotic Bax bands, as well as activation of the caspase-3 enzyme was shown in Western blot analysis. Phytochemical analysis suggested that hetisine type diterpene alkaloids were probably responsible for apoptotic activities. Conclusively, the present study demonstrated that D. semibarbatum alkaloid content exerted antiproliferative effects against prostate cancer cells by inducing the intrinsic pathway of apoptosis. © 2021, Briefland. All rights reserved.
Related Docs
View other Related Docs
1. Phytochemical Analysis of Daphne Pontica L. Stems With Their Pro-Apoptotic Properties Against Du-145 and Lncap Prostate Cancer Cells, DARU, Journal of Pharmaceutical Sciences (2022)
2. Chemistry, Biological Activities and Toxic Effects of Alkaloidal Constituents of Genus Delphinium - a Mini Review, Journal of HerbMed Pharmacology (2021)
3. Molecular Mechanism of Apoptosis Induction by Gaillardin, a Sesquiterpene Lactone, in Breast Cancer Cell Lines: Gaillardin-Induced Apoptosis in Breast Cancer Cell Lines, Cell Biology and Toxicology (2015)
4. Centaurea Cyanus Extracted 13-O-Acetylsolstitialin a Decrease Bax/Bcl-2 Ratio and Expression of Cyclin D1/Cdk-4 to Induce Apoptosis and Cell Cycle Arrest in Mcf-7 and Mda-Mb-231 Breast Cancer Cell Lines, Journal of Cellular Biochemistry (2019)
5. Mitochondrial Pro-Apoptotic Properties of Sinularia Compressa From Persian Gulf Against Breast Cancer Cells and Its Chemical Composition, Nutrition and Cancer (2022)
6. A Comparison of Thiazolyl Blue (Mtt) Versus Sulforhodamine B (Srb) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, Ags, and Pc3 Cancer Cell Lines, Journal of Isfahan Medical School (2017)
7. Dihydroartemisinin Enhances the Therapeutic Efficacy of Bh3 Mimetic Inhibitor in Acute Lymphoblastic Leukemia Cells Via Inhibition of Mcl-1, Asian Pacific Journal of Cancer Prevention (2024)
8. Cytotoxicity and Apoptosis Inducing Effects of Some Lathyrane and Tigliane Diterpenes Against Breast Cancer Cell Lines, Traditional and Integrative Medicine (2021)
Experts (# of related papers)
View all Related Experts
Mahmoud Aghaei (19)
Mustafa Ghanadian (13)
Other Related Docs
9. Persicoimidate Isolated From Allium Ampeloprasum Subsp. Persicum With Apoptotic Effects Against Breast Cancer Cell Lines, Iranian Journal of Pharmaceutical Sciences (2020)
10. Persianolide-A, an Eudesmanolide-Type Sesquiterpene Lactone From Artemisia Kopetdaghensis, Induces Apoptosis by Regulating Erk Signaling Pathways, Research in Pharmaceutical Sciences (2024)
11. Evaluation of Anticancer Effects of Cerium Oxide Nanoparticles on Mouse Fibrosarcoma Cell Line, Journal of Cellular Physiology (2019)
12. Bioactivity-Guided Isolation of New Antiproliferative Compounds From Juniperus Foetidissima Willd, Natural Product Research (2016)
13. A New Taraxastane Triterpene From Euphorbia Denticulata With Cytotoxic Activity Against Prostate Cancer Cells, Iranian Journal of Pharmaceutical Research (2018)
14. Apoptosis-Inducing Effect of Spiroaminopyrimidine Analogue in Nb4 Leukemia Cells Via Down-Regulation of Birc5 Expression, Experimental Oncology (2019)
15. Novel Tocopherol Succinate-Polyoxomolybdate Bioconjugate As Potential Anti-Cancer Agent, Journal of Inorganic and Organometallic Polymers and Materials (2021)
16. The Effects of Abt-199 and Dihydroartemisinin Combination on Cell Growth and Apoptosis in Human U937 and Kg-1 Cancer Cells, Asian Pacific Journal of Cancer Prevention (2024)
17. Α2β1 Integrin Specific Inhibitor Btt-3033 Promotes Paclitaxel-Induced Apoptosis in Human Ovarian Cancer Cells, Research in Pharmaceutical Sciences (2024)
18. A3 Adenosine Receptor Agonist Inhibited Survival of Breast Cancer Stem Cells Via Gli-1 and Erk1/2 Pathway, Journal of Cellular Biochemistry (2017)
19. Adenosine Induces Cell-Cycle Arrest and Apoptosis in Androgen-Dependent And-Independent Prostate Cancer Cell Lines, Lncap-Fgc-10, Du-145, and Pc3, Prostate (2012)
20. Comparison of Anticancer Effects of Hydroalcoholic Extracts of Camellia Sinensis and Lepidium Sativum L on Hela Cell Line, International Journal of Cancer Management (2020)
21. Two New Dammarane Triterpenes Isolated From Cleome Khorassanica Bunge & Bien With Cytotoxicity Against Du-145 and Lncap Prostate Cancer Cell Lines, Journal of Asian Natural Products Research (2020)
22. Enhancing Prostate Cancer Cells’ Sensitivity to Flutamide by Resveratrol: An In-Vitro Study, Tissue and Cell (2025)
23. Crocin Effects on Human Myeloma Cells Regarding Intracellular Redox State, Dna Fragmentation, and Apoptosis or Necrosis Profile, Jundishapur Journal of Natural Pharmaceutical Products (2014)
24. Formononetin and Dihydroartemisinin Act Synergistically to Induce Apoptosis in Human Acute Myeloid Leukemia Cell Lines, Cell Journal (2024)
25. Mitochondrial and Caspase Pathways Are Involved in the Induction of Apoptosis by Nardosinen in Mcf-7 Breast Cancer Cell Line, Research in Pharmaceutical Sciences (2018)
26. Cytotoxic Effect of Drimia Maritima Bulb Extract and Induction of Mitochondrial Apoptotic Signaling in Human Breast Cancer Cells, Mcf-7 and Mda-Mb-468, OncoTargets and Therapy (2018)
27. Britannin, a Sesquiterpene Lactone, Inhibits Proliferation and Induces Apoptosis Through the Mitochondrial Signaling Pathway in Human Breast Cancer Cells, Tumor Biology (2015)
28. The Modulatory Effects of Two Bioflavonoids, Quercetin and Thymoquinone on the Expression Levels of Dna Damage and Repair Genes in Human Breast, Lung and Prostate Cancer Cell Lines, Pathology Research and Practice (2022)
29. Jatropha-6(17),11 E -Diene Class Derivatives Induce Apoptosis Effects in Ovcar-3 and Caov-4 Ovarian Cancer Cell Lines Via a Mitochondrial Pathway, Biochemistry and Cell Biology (2017)
30. Apoptosis and Cell Cycle Regulatory Effects of Adenosine by Modulation of Gli-1 and Erk1/2 Pathways in Cd44+ and Cd24− Breast Cancer Stem Cells, Cell Proliferation (2017)
31. The Cytotoxicity of 27-Hydroxycholesterol in Mcf-7 and Mda-Mb-231, Advanced Biomedical Research (2023)
32. New 6(17)-Epoxylathyrane Diterpene: Aellinane From Euphorbia Aellenii Induces Apoptosis Via Mitochondrial Pathway in Ovarian Cancer Cell Line, Toxicology Mechanisms and Methods (2017)
33. Galectin-9 Induces Apoptosis in Ovcar-3 Ovarian Cancer Cell Through Mitochondrial Pathway, Research in Pharmaceutical Sciences (2018)
34. Dihydroartemisinin Induces Apoptosis in Human Bladder Cancer Cell Lines Through Reactive Oxygen Species, Mitochondrial Membrane Potential, and Cytochrome C Pathway, International Journal of Preventive Medicine (2017)
35. Induction of G2/M Phase Arrest and Apoptosis by a New Tetrahydroingenol Diterpenoid From Euphorbia Erythradenia Bioss. in Melanoma Cancer Cells, Biomedicine and Pharmacotherapy (2017)