Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effects of Abt-199 and Dihydroartemisinin Combination on Cell Growth and Apoptosis in Human U937 and Kg-1 Cancer Cells Publisher Pubmed



Nazmabadi R1 ; Pooladi M1, 2 ; Amri J3 ; Darvish M1 ; Abbasi Y4 ; Karami H1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Molecular Medicine and Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
  2. 2. Department of Anatomy, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Anatomy, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran

Source: Asian Pacific Journal of Cancer Prevention Published:2024


Abstract

Introduction: Change in the balance of Bcl-2 family proteins is one of the main reasons for resistance of tumor cells to ABT-199. In this study, the effect of dihydroartemisinin on cell growth, apoptosis and sensitivity of the AML cells to ABT-199 was investigated. Methods: Cell proliferation and survival were assessed by trypan blue staining and MTT assay, respectively. Cell apoptosis was measured by Hoechst 33342 staining and caspase-3 activity assay. The expression levels of Bcl-2, Mcl-1 and Bax mRNA were tested by qRT-PCR. Results: Our data showed that combination therapy significantly reduced the IC50 value and synergistically decreased the AML cell survival and growth compared with dihydroartemisinin or ABT-199 alone. Treatment with each of ABT-199 or dihydroartemisinin alone clearly enhanced the Bax mRNA expression and inhibited the expression of Mcl-1 and Bcl-2 mRNA. Inhibition of Mcl-1 mRNA by dihydroartemisinin was associated with enhancement of apoptosis induced by ABT-199 in AML cells. Conclusion: In conclusion, dihydroartemisinin not only triggers the intrinsic pathway of apoptosis, but also can increase the sensitivity of the AML cells to ABT-199 via suppression of Mcl-1 expression. © (2024) This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License.
Related Docs
View other Related Docs
1. Dihydroartemisinin Enhances the Therapeutic Efficacy of Bh3 Mimetic Inhibitor in Acute Lymphoblastic Leukemia Cells Via Inhibition of Mcl-1, Asian Pacific Journal of Cancer Prevention (2024)
2. Formononetin and Dihydroartemisinin Act Synergistically to Induce Apoptosis in Human Acute Myeloid Leukemia Cell Lines, Cell Journal (2024)
3. A Comparison of Thiazolyl Blue (Mtt) Versus Sulforhodamine B (Srb) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, Ags, and Pc3 Cancer Cell Lines, Journal of Isfahan Medical School (2017)
4. Sar131675 Receptor Tyrosine Kinase Inhibitor Induces Apoptosis Through Bcl-2/Bax/Cyto C Mitochondrial Pathway in Human Umbilical Vein Endothelial Cells, Anti-Cancer Agents in Medicinal Chemistry (2022)
5. Persianolide-A, an Eudesmanolide-Type Sesquiterpene Lactone From Artemisia Kopetdaghensis, Induces Apoptosis by Regulating Erk Signaling Pathways, Research in Pharmaceutical Sciences (2024)
6. Effects of Disulfiram on Apoptosis in Panc-1 Human Pancreatic Cancer Cell Line, Research in Pharmaceutical Sciences (2014)
7. Apoptosis-Inducing Effect of Spiroaminopyrimidine Analogue in Nb4 Leukemia Cells Via Down-Regulation of Birc5 Expression, Experimental Oncology (2019)
8. Cycloartane Triterpenoid From Euphorbia Macrostegia Modulates Er Stress Signaling Pathways to Induce Apoptosis in Mda-Mb231 and Mcf-7 Breast Cancer Cell Lines, Naunyn-Schmiedeberg's Archives of Pharmacology (2023)
Experts (# of related papers)
View all Related Experts
Mahmoud Aghaei (18)
Mustafa Ghanadian (7)
Other Related Docs
9. Α2β1 Integrin Specific Inhibitor Btt-3033 Promotes Paclitaxel-Induced Apoptosis in Human Ovarian Cancer Cells, Research in Pharmaceutical Sciences (2024)
10. Evaluation of Antitumor Effects of Aspirin and Lgk974 Drugs on Cellular Signaling Pathways, Cell Cycle and Apoptosis in Colorectal Cancer Cell Lines Compared to Oxaliplatin Drug, Fundamental and Clinical Pharmacology (2020)
11. Synergistic Anti-Proliferative Effects of Lenalidomide and Dexamethasone on the Ht-29 Cell Line Through Apoptotic Genes, International Journal of Cancer Management (2021)
12. Adenosine Induces Cell-Cycle Arrest and Apoptosis in Androgen-Dependent And-Independent Prostate Cancer Cell Lines, Lncap-Fgc-10, Du-145, and Pc3, Prostate (2012)
13. Myricetin Apoptotic Effects on T47d Breast Cancer Cells Is a P53-Independent Approach, Asian Pacific Journal of Cancer Prevention (2020)
14. Mitochondrial and Caspase Pathways Are Involved in the Induction of Apoptosis by Nardosinen in Mcf-7 Breast Cancer Cell Line, Research in Pharmaceutical Sciences (2018)
15. Synergistic Antitumor Effect of Nvp-Bez235 and Cape on Mda-Mb-231 Breast Cancer Cells, Biomedicine and Pharmacotherapy (2017)
16. Pd-1/Pd-L1 Interaction Regulates Bcl2, Ki67, Bax, and Casp3, Altering Proliferation, Survival, and Apoptosis in Acute Myeloid Leukemia, Iranian Journal of Allergy, Asthma and Immunology (2023)
17. Cytotoxic Effect of Drimia Maritima Bulb Extract and Induction of Mitochondrial Apoptotic Signaling in Human Breast Cancer Cells, Mcf-7 and Mda-Mb-468, OncoTargets and Therapy (2018)
18. Britannin, a Sesquiterpene Lactone, Inhibits Proliferation and Induces Apoptosis Through the Mitochondrial Signaling Pathway in Human Breast Cancer Cells, Tumor Biology (2015)
19. Effect of Hepatocyte Growth Factor (Hgf) on the Level of Survivin & Xiap Expression in Several Human Cancer Cell Lines, After Treating With Dna Damaging Agent, Molecular and Cellular Biochemistry (2007)
20. Activation of A2b Adenosine Receptor Regulates Ovarian Cancer Cell Growth: Involvement of Bax/Bcl-2 and Caspase-3, Biochemistry and Cell Biology (2015)
21. An Immunohistochemistry Study of Tissue Bcl-2 Expression and Its Serum Levels in Breast Cancer Patients, Annals of the New York Academy of Sciences (2008)
22. New 6(17)-Epoxylathyrane Diterpene: Aellinane From Euphorbia Aellenii Induces Apoptosis Via Mitochondrial Pathway in Ovarian Cancer Cell Line, Toxicology Mechanisms and Methods (2017)
23. The Role of Strain Variation in Bax and Bcl-2 Expression in Murine Bleomycin-Induced Pulmonary Fibrosis, Pakistan Journal of Biological Sciences (2008)
24. Adenosine Induces Cell Cycle Arrest and Apoptosis Via Cyclind1/Cdk4 and Bcl-2/Bax Pathways in Human Ovarian Cancer Cell Line Ovcar-3, Tumor Biology (2013)
25. All-Trans Retinoic Acid and Genistein Induce Cell Apoptosis in Ovcar-3 Cells by Increasing the P14 Tumor Suppressor Gene, Research in Pharmaceutical Sciences (2016)
26. Anticancer Activity of Delphinium Semibarbatum Alkaloid Fractions Against Lncap, and Du 145 Human Prostate Cancer Cells Through the Intrinsic Apoptotic Pathway, Iranian Journal of Pharmaceutical Research (2021)
27. Mitochondrial and Caspase Pathways Are Involved in the Induction of Apoptosis by Ib-Meca in Ovarian Cancer Cell Lines, Tumor Biology (2014)
28. Implementation of Docking, Molecular Dynamics and Free Energy to Investigate Drug Potency of Novel Bcr-Ablt315i Inhibitors As an Alternative to Ponatinib, Computational Toxicology (2021)
29. Molecular Mechanisms of A3 Adenosine Receptor-Induced G1 Cell Cycle Arrest and Apoptosis in Androgen-Dependent and Independent Prostate Cancer Cell Lines: Involvement of Intrinsic Pathway, Journal of Cancer Research and Clinical Oncology (2011)
30. Molecular Mechanism of Apoptosis Induction by Gaillardin, a Sesquiterpene Lactone, in Breast Cancer Cell Lines: Gaillardin-Induced Apoptosis in Breast Cancer Cell Lines, Cell Biology and Toxicology (2015)
31. The Role of Bcl-2 Family Proteins in Pulmonary Fibrosis, European Journal of Pharmacology (2014)
32. Centaurea Cyanus Extracted 13-O-Acetylsolstitialin a Decrease Bax/Bcl-2 Ratio and Expression of Cyclin D1/Cdk-4 to Induce Apoptosis and Cell Cycle Arrest in Mcf-7 and Mda-Mb-231 Breast Cancer Cell Lines, Journal of Cellular Biochemistry (2019)
33. Dihydroartemisinin Induces Apoptosis in Human Bladder Cancer Cell Lines Through Reactive Oxygen Species, Mitochondrial Membrane Potential, and Cytochrome C Pathway, International Journal of Preventive Medicine (2017)
34. Induction of G2/M Phase Arrest and Apoptosis by a New Tetrahydroingenol Diterpenoid From Euphorbia Erythradenia Bioss. in Melanoma Cancer Cells, Biomedicine and Pharmacotherapy (2017)