Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Correlation Between Sperm Motility and Sperm Chromatin/Dna Damage Before and After Cryopreservation and the Effect of Folic Acid and Nicotinic Acid on Post-Thaw Sperm Quality in Normozoospermic Men Publisher Pubmed



Rarani FZ1 ; Golshaniranpour F1, 2 ; Dashti GR1, 2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Saint Maryam Fertility and Infertility Center, Shahid Beheshti Hospital, Isfahan, Iran

Source: Cell and Tissue Banking Published:2019


Abstract

Cryopreservation exposes sperm to physical and chemical stresses causing cell damages and impairs sperm functions. The aim of this study was to evaluate the association between motility and sperm chromatin/DNA damage before and after cryopreservation and investigate the effects of folic acid and nicotinic acid on post-thaw sperm quality. Thirty semen samples were obtained from 30 normozoospermic men, aged between 25 and 45 years old. Each sample were divided into five aliquots to form the following groups: fresh, cryopreserved with sperm-freeze only (control), with nicotinic acid (10 mM), with folic acid (50 nM), and with a combination of folic acid (50 nM) + nicotinic acid (10 mM). Sperm viability and motility in each group were assessed by eosin-nigrosine staining and computer-aided sperm analysis respectively. Sperm chromatin quality was studied by aniline blue, toluidine blue, acridine orange staining methods and sperm chromatin dispersion test. Cryopreservation led to a significant reduction in sperm quality in comparison to fresh sample groups (p < 0.05). Sperm chromatin damage was negatively correlated with the percentage of progressively motile cells. Supplementation of the cryopreservation medium with folic acid or nicotinic acid induced a significant improvement in sperm parameters and chromatin quality, compared to control groups (p < 0.05). Meanwhile, the combination of folic acid + nicotinic acid showed a significant protective effect in post thaw sperm. In conclusion, cryopreservation generated oxidative stress, inducingsperm cryodamage, reducing progressive motility and sperm quality, as an indicator of significant chromatin/DNA damage. Folic acid and nicotinic acid exhibited a potential cryoprotective effect by enhancing sperm quality. © 2019, Springer Nature B.V.
Related Docs
View other Related Docs
1. Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men During Cryopreservation., Scientific Journal of Kurdistan University of Medical Sciences (2022)
2. Effects of Biotin and Folic Acid on Motility, Viability, Morphology, Chromatin Density and Integrity of Cryopreserved and Thawed Sperm in Normozoospermic Men, Scientific Journal of Kurdistan University of Medical Sciences (2021)
3. Effect of Chromatin Condensation on Frozen-Thawed Sperm Dna Integrity in Normozoospermic Men, Scientific Journal of Kurdistan University of Medical Sciences (2019)
4. The Effect of Folic Acid and Nicotinic Acid on Malondialdehyde Levels of Semen in Oligospermia Men After Cryopreservation, Journal of Isfahan Medical School (2021)
5. Comparison Between Open and Closed Systems for Vitrification of Individual Sperm: Assessing Morphometric Measurements and Chromatin Integrity, Middle East Fertility Society Journal (2024)
6. The Role of Sperm Chromatin Anomalies on the Outcome of Assisted Reproductive Techniques, Yakhteh (2006)
7. Effects of Keeping Semen Samples in 4-6 °C on Sperm Motility, Viability and Dna Denaturation, Journal of Isfahan Medical School (2016)
8. The Correlation Between Sperm Dna Fragmentation and the Expression of Long Non-Coding Rna Hotair in Infertile Men, Journal of Isfahan Medical School (2021)
Experts (# of related papers)
View all Related Experts
Shahnaz Razavi (29)
Mohammad Mardani (16)
Other Related Docs
9. Effects of Temperature and Storage Time on the Motility, Viability, Dna Integrity and Apoptosis of Processed Human Spermatozoa, Andrologia (2020)
10. Does Folate Deficiency Alter Sperm Parameters in Infertile Males?, Immunopathologia Persa (2024)
11. The Effects of Vitamin C and Menthone on Acyclovir Induced Dna Damage in Rat Spermatozoa: An Experimental Study, International Journal of Reproductive BioMedicine (2018)
12. Evaluation of Sperm Selection Procedure Based on Hyaluronic Acid Binding Ability on Icsi Outcome, Journal of Assisted Reproduction and Genetics (2008)
13. Relationship Between Protamine Deficiency With Fertilization Rate and Incidence of Sperm Premature Chromosomal Condensation Post-Icsi, Andrologia (2004)
14. Effect of Saffron on Rat Sperm Chromatin Integrity, Iranian Journal of Reproductive Medicine (2014)
15. Optimizing Acridine Orange Staining for Assessment of Protective Effects of Saffron and Vitamin E on Rat Sperm Dna Structure, Journal of Isfahan Medical School (2014)
16. The Epididymal Sperm Viability, Motility and Dna Integrity in Dead Mice Maintained at 4-6°C, Iranian Journal of Reproductive Medicine (2013)
17. Relationship Between Long Non-Coding Rna Malat1 and Hotair Expression With Sperm Parameters, Dna and Malondialdehyde Levels in Male Infertility, Tissue and Cell (2023)
18. Evaluation of Zeta and Ha-Binding Methods for Selection of Spermatozoa With Normal Morphology, Protamine Content and Dna Integrity, Andrologia (2010)
19. Relation Between Different Human Sperm Nuclear Maturity Tests and in Vitro Fertilization, Journal of Assisted Reproduction and Genetics (2001)
20. Can Sperm Protamine Deficiency Induce Sperm Premature Chromosomal Condensation?, Andrologia (2006)
21. Comparing the Roles of Sperm Chromatin Integrity and Apoptosis in Intrauterine Insemination Outcomes of Couples With Mild Male and Female Factor Infertility, Journal of Reproduction and Infertility (2014)
22. Evaluation of Nrf2/Are Signaling Pathway in the Presence of Pentoxifylline As a Cryoprotectant in Mouse Spermatogonial Stem Cells, Biopreservation and biobanking (2023)
23. Effect of Human Sperm Chromatin Anomalies on Fertilization Outcome Post-Icsi, Andrologia (2003)
24. Improvement of Rat Sperm Chromatin Integrity and Spermatogenesis With Omega 3 Following Bleomycin, Etoposide and Cisplatin Treatment, Nutrition and Cancer (2021)
25. Effect of Polyvinyl Pyrrolidone on Sperm Membrane Integrity and Chromatin Status, International Journal of Fertility and Sterility (2008)
26. Assessment of the Changing Trend of Different Kinds of Sperm Motility During the Storage of Normozoospermic Men’S Semen Samples at 4-6 ºc, Journal of Isfahan Medical School (2017)
27. The Association Between Serum Vitamin D Level and Sperm Parameters: A Pilot Study in a Subset of Iranian Infertile Males, Immunopathologia Persa (2020)
28. The Methylation Status of Gata3 Potentially Predicts the Outcomes of Assisted Reproductive Technologies, Human Fertility (2023)
29. Dietary Fats, Minerals and Semen Quality and Quantity Among Men Attending a Fertility Clinic: A Cross-Sectional Study in Iran, Journal of Nutrition and Food Security (2022)
30. Effect of Varicocelectomy on Semen Parameters and Protamine Deficiency 3 and 6 Months Post Surgery, Journal of Isfahan Medical School (2007)
31. The Association of Food Pattern and Sperm Quality Parameters and Biochemical Markers in Infertile Men: A Review Study, Journal of Health System Research (2020)
32. Assessment of Adiponectin and Sperm Function Parameters in Obese and Non-Obese: A Comprehensive Study, Cell Journal (2022)
33. The Relationship of Oocyte and Sperm Membrane Binding Protein With the Success of Intrauterine Insemination, Journal of Isfahan Medical School (2014)
34. Can Zeta Sperm Selection Method, Recover Sperm With Higher Dna Integrity Compare to Density Gradient Centrifugation?, Iranian Journal of Reproductive Medicine (2009)
35. Role of Dietary Antioxidants and Vitamins Intake in Semen Quality Parameters: A Cross-Sectional Study, Clinical Nutrition ESPEN (2022)
36. Influence of Sperm Chromatin Anomalies on Assisted Reproductive Technology Outcome, Fertility and Sterility (2009)
37. Sperm Mtt Viability Assay: A New Method for Evaluation of Human Sperm Viability, Journal of Assisted Reproduction and Genetics (2002)
38. Investigation of Association Between Genetic Diversities in Yq11.223 Region With Men Infertility in Isfahan Population, Journal of Isfahan Medical School (2011)
39. Effect of Zinc on Spermatogenesis and Sperm Chromatin Condensation in Bleomycin, Etoposide, Cisplatin Treated Rats, Cell Journal (2019)
40. Gonadotropins in Infertile Men With Idiopathic Hypogonadotropic Hypogonadism, International Journal of Fertility and Sterility (2008)
41. Selection of Sperm Based on Combined Density Gradient and Zeta Method May Improve Icsi Outcome, Human Reproduction (2009)
42. Failed Fertilization After Icsi and Spermiogenic Defects, Fertility and Sterility (2008)
43. Effects of Low Doses of Exogenous Thymoquinone on Sperm Motility and Viability of Normozoospermic Men, Journal of Isfahan Medical School (2014)
44. Investigating the Correlation Between Ubiquitination With Motility, Morphology, and Dna Methylation in Rat Sperm, Journal of Isfahan Medical School (2017)
45. The Effect of Consecutive Ejaculation on the Sperm Parameters in the Oligo-Astheno-Teratozoospermia (Oat) Men, Zygote (2025)
46. Is There a Relationship Between Serum Vitamin D and Semen Parameters? a Cross-Sectional Sample of the Iranian Infertile Men, Basic and Clinical Andrology (2021)
47. Correlation of Obesity and Serum Vitamin D Levels With Sperm Dna Integrity, Sperm Quality, and Sperm Viability in Normozoospermia Men, Advanced Biomedical Research (2022)
48. Effect of Preservation of Human Semen Sample at 4–6 and 25 °C on Sperm Motility, Cell and Tissue Banking (2018)
49. Treatment Outcome Following Intracytoplasmic Injection of Sperm Retrieved From Ejaculate, Epididymis, or Testis of Infertile Men, Archives of Iranian Medicine (2004)
50. Efficiency of Sil-Select and Percoll to Recover Spermatozoa With Normal Chromatin and Morphology and the Effect of These Parameters on Fertilization, Embryo Quality and Cleavage Score, Middle East Fertility Society Journal (2003)