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Cloning of Iranian Leishmania Major Mannose-1-Phosphate Guanyltransferase Gene



Hejazi SH1 ; Ziajahromi N2 ; Bandehpour M3 ; Eslami G1 ; Salehi R4 ; Khamesipour A5 ; Kazemi B3
Authors
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Authors Affiliations
  1. 1. Parasitology and Mycology Department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Islamic Azad University, Science and Research Campus, Tehran, Iran
  3. 3. Cellular and Molecular Biology Research Center, Shahid Beheshti University, M. C., Tehran, Iran
  4. 4. Genetic and Molecular Biology Department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Center of Research in Skin Diseases and Leprosy, Tehran University of Medical Science, Tehran, Iran

Source: African Journal of Biotechnology Published:2009

Abstract

Leishmania is an obligatory intracellular protozoan parasite which infects human beings when sand fly vector takes a blood meal. Most efforts are towards designing an effective vaccine to prevent Leishmanasis. In this way, development of candidate antigen for vaccine has special importance. The gene encoding mannose-1-phosphate guanyltransferase has 1140 bp and is on chromosome 23. In eukaryotes, mannose is a monosaccharide used for the glycosylation of lipids and proteins. In the absent of the enzyme, they are unable to synthesize mannose-containing glycoconjugates such as protein and lipid. In this study, we cloned mannose-1-phosphate guanyltransferase gene of Iranian L. major in pET32a expression vector. © 2009 Academic Journals.
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