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Evaluation of the Anti-Leishmanial Effect of Recombinant Clostridium Α-Toxin Publisher



Namdar F1 ; Khanahmad H2 ; Ghayour Z1 ; Mirzaei F3 ; Namdar A4 ; Aghaei M5 ; Izadi S6 ; Khamesipour F7, 8 ; Hejazi SH9
Authors
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Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  4. 4. Department of Community Medicine, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
  5. 5. Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
  8. 8. Shahid Beheshti University of Medical Sciences, Tehran, Iran
  9. 9. Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Infection and Drug Resistance Published:2020


Abstract

Background: Leishmaniasis is an infectious disease common in tropical and subtropical regions caused by the genus Leishmania, which is transmitted by the bite of female sandflies. In this study, we evaluate the anti-leishmanial effect of recombinant Clostridium α-toxin protein alone and the combination with glucantime through in vitro and in vivo. Materials and Methods: Production, expression, and purification of recombinant α-toxin were evaluated by SDS-PAGE and Western blotting techniques. The antileishmanial activ-ities of the purified α-toxin plus and without glucantime were examined in vitro and in vivo. Results: The results indicated successful expression of α-toxin as a 48 kDa band on SDS-PAGE and Western blot methods. Also, evaluation of α-toxin IC50 showed the strong fatal effect of it, and glucantime on medium proliferated Leishmania promastigotes at lower concentrations compared with glucantime or α-toxin alone. Moreover, in vivo surveys showed that at the end of treatment courses, the mean of lesion size diminished in glucantime plus α-toxin treated mice versus negative control groups (p < 0.001). Also, there was a significant difference in the parasite burden of the spleen and liver of the control versus the test groups (p < 0.001). Conclusion: The results showed recombinant α-toxin has synergistic effects with glucan-time in destroying Leishmania parasites. © 2020 Namdar et al.
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