Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Amino Acid Substitutions in Erg11p of Azole-Resistant Candida Glabrata: Possible Effective Substitutions and Homology Modelling Publisher Pubmed



Nabili M1 ; Abdollahi Gohar A2 ; Badali H2 ; Mohammadi R3 ; Moazeni M2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Mazandaran University of Medical Sciences, Sari, Iran
  2. 2. Invasive Fungi Research Centre, Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, 20th km of Khazar Abad Road, Sari, Iran
  3. 3. Department of Medical Parasitology and Mycology, School of Medicine and Infectious Diseases, Tropical Medicine Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Global Antimicrobial Resistance Published:2016


Abstract

Understanding the mechanisms responsible for fluconazole resistance in Candida glabrata is not only crucial for the development of new antifungals but is also important in choosing appropriate antifungals for patients at the earliest stages. The aim of this study was to determine the Erg11p amino acid substitutions in fluconazole-resistant C. glabrata isolates. Sixty clinical isolates of C. glabrata were investigated. In vitro antifungal activities of fluconazole, itraconazole and voriconazole were determined using the broth microdilution reference method. The ERG11 gene for resistant (n = 4) and susceptible (n = 1) isolates were sequenced and multi-aligned using MEGA6 software. A homology model of the C. glabrata ERG11 gene was created by SWISS-MODEL software using the crystal structure of Saccharomyces cerevisiae Erg11p as a template, and the predicted binding sites to fluconazole were investigated. Fluconazole and multi-azole resistance were observed in 6.7% and 3.3% of the isolates, respectively. Several amino acid substitutions were identified, among which some were also identified in susceptible isolates. The amino acid substitution G236V was at the binding site, and substitutions H146Q and D234E were near to the binding site of triazoles according to the SWISS-MODEL. According to the homology modelling results, the amino acid substitution G236V is highly likely to play a key role in azole resistance development. © 2016 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.
Related Docs
View other Related Docs
1. Detection of Erg11 Point Mutations in Iranian Fluconazole-Resistant Candida Albicans Isolates, Current Medical Mycology (2019)
2. Low Level of Antifungal Resistance in Iranian Isolates of Candida Glabrata Recovered From Blood Samples in a Multicenter Study From 2015 to 2018 and Potential Prognostic Values of Genotyping and Sequencing of Pdr1, Antimicrobial Agents and Chemotherapy (2019)
3. Caspofungin-Non-Susceptible Candida Orthopsilosis Isolated From Onychomycosis in Iran, Iranian Journal of Public Health (2017)
4. Regulation of Erg3, Erg6, and Erg11 Genes in Antifungal-Resistant Isolates of Candida Parapsilosis, Iranian Biomedical Journal (2017)
5. In Vitro Activities of Six Antifungal Drugs Against Candida Glabrata Isolates: An Emerging Pathogen, Jundishapur Journal of Microbiology (2016)
6. The Evaluation of the Overexpression of the Erg-11, Mdr-1, Cdr-1, and Cdr-2 Genes in Fluconazole-Resistant Candida Albicans Isolated From Ahvazian Cancer Patients With Oral Candidiasis, Journal of Clinical Laboratory Analysis (2022)
7. An Alarming Rise of Non-Albicans Candida Species and Uncommon Yeasts in the Clinical Samples; a Combination of Various Molecular Techniques for Identification of Etiologic Agents, BMC Research Notes (2019)
8. Antifungal Susceptibility Analysis of Clinical Isolates of Candida Parapsilosis in Iran, Iranian Journal of Public Health (2016)
Experts (# of related papers)
View all Related Experts
Rasoul Mohammadi (30)
Seyed Hossein Mirhendi Esfahani (27)
Other Related Docs
9. A 9-Year Experience of Aspergillus Infections From Isfahan, Iran, Infection and Drug Resistance (2020)
10. Frequency of Uncommon Clinical Yeast Species Confirmed by Its-Sequencing, Archives of Clinical Infectious Diseases (2019)
11. Antifungal Activity of Anethum Graveolens Extract and Atrovastatin Against Candida Species Compared to Fluconazole, Journal of Isfahan Medical School (2015)
12. In Vitro Activities of Five Antifungal Drugs Against Opportunistic Agents of Aspergillus Nigri Complex, Mycopathologia (2016)
13. In Vitro Activity of Juglone (5-Hydroxy-1,4-Naphthoquinone) Against Both Fluconazole-Resistant and Susceptible Candida Isolates; [Actividad in Vitro De Juglona (5-Hidroxi-1,4-Naftoquinona) Contra Aislamientos De Candida Resistentes Y Sensibles a Fluconazol], Revista Iberoamericana de Micologia (2022)
14. Species Distribution and Antifungal Susceptibility Profile of Candida Isolates From Blood and Other Normally Sterile Foci From Pediatric Icu Patients in Tehran, Iran, Medical Mycology (2020)
15. A 3-Year Study of Candida Infections Among Patients With Malignancy: Etiologic Agents and Antifungal Susceptibility Profile, Frontiers in Cellular and Infection Microbiology (2023)
16. In Vitro Antifungal Susceptibility Pattern of Candida Species Isolated From Gastroesophageal Candidiasis, Gastroenterology and Hepatology from Bed to Bench (2021)
17. Antifungal Activity of a Novel Triazole, Efinaconazole and Nine Comparators Against 354 Molecularly Identified Aspergillus Isolates, Mycopathologia (2020)
18. In-Vivo Efficiency of the Novel Azole Compounds (Attaf-1 and Attaf-2) Against Systemic Candidiasis in a Murine Model, Journal of Medical Mycology (2023)
19. Utilization of Size Polymorphism in Its1 and Its2 Regions for Identification of Pathogenic Yeast Species, Journal of Medical Microbiology (2017)
20. Epidemiology and Antifungal Susceptibility of Candida Species Isolated From 10 Tertiary Care Hospitals in Iran, Microbiology Spectrum (2022)
21. Confirmation of Fifth Candida Auris Clade by Whole Genome Sequencing, Emerging Microbes and Infections (2022)
22. Quantitative Analysis of Single-Nucleotide Polymorphism for Rapid Detection of Tr34/L98h- and Tr46/Y121f/T289a-Positive Aspergillus Fumigatus Isolates Obtained From Patients in Iran From 2010 to 2014, Antimicrobial Agents and Chemotherapy (2016)
23. Antifungal Effect of Atorvastatin Against Candida Species in Comparison to Fluconazole and Nystatin, Medicine and Pharmacy Reports (2019)
24. Species Identification and in Vitro Antifungal Susceptibility Testing of Aspergillus Section Nigri Strains Isolated From Otomycosis Patients, Journal de Mycologie Medicale (2018)
25. Characterization of Beta-Tubulin Dna Sequences Within Candida Parapsilosis Complex, Current Medical Mycology (2018)
26. In Vitro Susceptibility Patterns of Clinically Important Trichophyton and Epidermophyton Species Against Nine Antifungal Drugs, Mycoses (2015)
27. Candida Africana and Candida Dubliniensis As Causes of Pediatric Candiduria: A Study Using Hwp1 Gene Size Polymorphism, AIMS Microbiology (2020)
28. A Review on Candida Auris, an Emerging Drug-Resistant Fungal Pathogen, Journal of Isfahan Medical School (2022)
29. Evaluation of Prevalence of New Candida Species (C. Orthopsilosis and C. Metapsilosis) Among C. Parapsilosis Group Isolated From Patients by Pcr-Rflp of Sadh Gene in Iran, Journal of Isfahan Medical School (2011)
30. Candidemia in Children Caused by Uncommon Species of Candida, Archives of Pediatric Infectious Diseases (2018)
31. Identification and Frequency of Candida Species in Patients With Different Forms of Candidiasis in Isfahan, Using Pcr-Rflp Method, Journal of Isfahan Medical School (2011)
32. In Vitro Activity of Two Nanoparticles on Clinical Isolates of Candida Parapsilosis, Showing Resistance Against Antifungal Agents in Children, Archives of Clinical Infectious Diseases (2017)
33. In Vitro Antifungal Susceptibility Profile of Iranian Fusarium Isolates: Emphasising on the Potent Inhibitory Effect of Efinaconazole Compared to Other Drugs, Mycoses (2023)
34. The Frequency of Oral Candidiasis in Children and Adolescents With Leukemia in Isfahan Province, Iran, Journal of Isfahan Medical School (2017)
35. Antifungal Activity of Almond (Prunus Amygdalus) Hull Extracts Against Clinical Isolates of Candida Albicans, Current Medical Mycology (2024)
36. Exploring the Potent Antifungal Activity of Mazuj and Ghalghaf Gall Extracts Against Three Candida Species and Conducting Compositional and Characterization Analysis of Their Extracts Using Hplc-Dad and Lc-Esi-Ms/Ms, Journal of Medicinal Plants and By-Products (2024)
37. Epidemiologic and Antifungal Susceptibility Profile of Candiduria Among Patients Hospitalized in the Nephrology and Intensive Care Unit Wards, Tehran, Iran, Jundishapur Journal of Microbiology (2022)
38. Synthetic Approaches and Structural Diversity of Triazolylbutanols Derived From Voriconazole in the Antifungal Drug Development, European Journal of Medicinal Chemistry (2022)
39. Candida Africana Vulvovaginitis: Prevalence and Geographical Distribution, Journal de Mycologie Medicale (2020)
40. Molecular Characterization of Candida Dubliniensis and Candida Albicans in the Oral Cavity of Drug Abusers Using Duplex Polymerase Chain Reaction, Current Medical Mycology (2018)
41. South Asian (Clade I) Candida Auris Meningitis in a Paediatric Patient in Iran With a Review of the Literature, Mycoses (2022)
42. Candida Palmioleophila Candidemia and Bacterial Co-Infection in a 3-Month-Old Infant With Biliary Atresia, Frontiers in Cellular and Infection Microbiology (2023)
43. Methods for Identification of Candida Auris, the Yeast of Global Public Health Concern: A Review, Journal de Mycologie Medicale (2019)
44. Multicenter Identification and Antifungal Susceptibility Patterns of Candida Species Isolated From Clinical Samples, Jundishapur Journal of Microbiology (2017)
45. The Frequency of Candiduria in Hospitalized Patients at Nephrology Department, Labbafinejad Hospital, Tehran, Iran, Journal of Isfahan Medical School (2017)
46. Non-Invasive Methods to Diagnose Fungal Infections in Pediatric Patients With Hematologic Disorders, Jundishapur Journal of Microbiology (2016)
47. Identification of Candida Species and Antifungal Susceptibility in Cancer Patients With Oral Lesions in Ahvaz, Southern West of Iran, Advanced Biomedical Research (2020)
48. Otomycosis Due to the Rare Fungi Talaromyces Purpurogenus, Naganishia Albida and Filobasidium Magnum, Mycopathologia (2020)
49. Specific Identification of Candida Glabrata Via Colorimetric Assay Based on Gold Nanoparticles, Journal of Isfahan Medical School (2015)
50. Molecular Identification of Candida Species Isolated From Gastrooesophageal Candidiasis in Tehran, Iran, Gastroenterology and Hepatology from Bed to Bench (2015)