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Determining the Frequency of Pathogenic Yeasts in Clinical Urine Samples and Tracking the Trichosporon Using Gold Nanoparticles; [تعیین فراوانی انواع مخمرهای بیماریزا در نمونههای بالینی ادرار و ردیابی مخمر ترایکوسپورونبا استفاده از نانوذرات طلا] Publisher



Zareie I1 ; Darani HY1 ; Dehghan P1
Authors
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Authors Affiliations
  1. 1. Department of Mycology and Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2024


Abstract

Background: Timely identification and treatment of trichosporonosis is consequential due to the severity of pathogenesis and different therapies for candidiasis and its high mortality. In this study, in addition to determining the abundance of this fungus in clinical urine samples using past experiences in the function of gold nanoparticles identification of this yeast based on serological methods for rapid detection of yeast in urine samples has been presented. Methods: 249 samples of people suspected of urinary fungal infection were analyzed using morphological and molecular PCR-RFLP methods. Antigenic suspension was prepared from different species of Trichosporon. This mixture was injected with a complete adjuvant to a healthy rabbit. After injecting four booster doses using incomplete adjuvant, blood was drawn from the rabbit. The anti-fungal antibody ability in the rabbit serum was confirmed using the ELISA method. Then, the antibody was placed chemically on the surface of gold nanoparticles, and the method's performance was investigated. Findings: C. glabrata and C. albicans species were the most common yeasts isolated. Among isolated yeasts, four cases of Trichosporon in urinary samples (1.6%) were reported. In this study, diabetes and corona disease were the underlying factors, and similar studies confirm the role of these factors. The serological method based on gold nanoparticles has been used in other studies to detect pathogens. Conclusion: The frequency of trichosporon in urine was 1.6% during the study period. In this study, diabetes and Coronavirus disease were the main predisposing factors, and similar studies confirm the role of these factors. The serological method based on gold nanoparticles has been used in other studies to detect pathogens. © 2024 Isfahan University of Medical Sciences(IUMS). All rights reserved.
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