Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Specific Identification of Candida Glabrata Via Colorimetric Assay Based on Gold Nanoparticles



Einloo A1 ; Dehghan P2 ; Saluti M3 ; Mirzaahmadi S4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Biology Research Center, Islamic Azad University, Zanjan Branch, Zanjan, Iran
  4. 4. Department of Genetics, School of Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran

Source: Journal of Isfahan Medical School Published:2015

Abstract

Background: Candida glabrata is one of the most common Candida species which is able to get resistant to antifungals. So, the rapid identification of it, seems to be necessary for early treatment of infections. The conventional methods for detection of Candida species are time-consuming and difficult. Today, using of nanoparticles with unique properties has been expanded. The purpose of this study was rapid identification of Candida glabrata using colorimetric assay based on gold nanoparticles. Methods: Candida glabrata was cultured in yeast extract peptone dextrose broth medium and DNA was extracted. The gene specific sequence (CAGL0M05005g) was determined from the Candida genetic information bank. For polymerase chain reaction (PCR), the primers and probe were designed via Oligo 7 software. The PCR product and probe was affected by denaturation and optimized binding temperatures. The gold nanoparticles were added and changed the color to be visual; by using ultraviolet-visible (UV-vis) spectrophotometer and transmission electron microscopy (TEM), they were confirmed. To evaluate the sensitivity of new method, different concentrations of PCR product were used and the results were analyzed using gel electrophoresis. Findings: The optimum temperatures of the primer binding to the DNA template and hybridization the probe was determined. After adding gold nanoparticles, the mixture solution color was changed from red to purple. The results were confirmed via spectroscopy and electron microscopy. Conclusion: The results indicated that in detection of Candida glabrata, the new method is faster than conventional methods and more sensitive than gel electrophoresis. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles, Journal of Isfahan Medical School (2022)
2. Cross-Sectional Study of Candidemia From Isfahan, Iran: Etiologic Agents, Predisposing Factors, and Antifungal Susceptibility Testing, Journal of Research in Medical Sciences (2021)
3. In Vitro Activities of Six Antifungal Drugs Against Candida Glabrata Isolates: An Emerging Pathogen, Jundishapur Journal of Microbiology (2016)
4. Methods for Identification of Candida Auris, the Yeast of Global Public Health Concern: A Review, Journal de Mycologie Medicale (2019)
5. The Matching Rate of the Frequency of the Isolated Candida Species Calculated by Morphological and Molecular Methods in Patients With Vaginitis, Journal of Isfahan Medical School (2017)
6. Determining the Frequency of Pathogenic Yeasts in Clinical Urine Samples and Tracking the Trichosporon Using Gold Nanoparticles; [تعیین فراوانی انواع مخمرهای بیماریزا در نمونههای بالینی ادرار و ردیابی مخمر ترایکوسپورونبا استفاده از نانوذرات طلا], Journal of Isfahan Medical School (2024)
7. Characterization of Beta-Tubulin Dna Sequences Within Candida Parapsilosis Complex, Current Medical Mycology (2018)
8. Differentiation of Candida Albicans Complex Species Isolated From Invasive and Non-Invasive Infections Using Hwp1 Gene Size Polymorphism, Current Medical Mycology (2021)
Experts (# of related papers)
View all Related Experts
Seyed Hossein Mirhendi Esfahani (21)
Rasoul Mohammadi (20)
Other Related Docs
9. Overview on the Infections Related to Rare Candida Species, Pathogens (2022)
10. Species Distribution and Susceptibility Profiles of Oral Candidiasis in Hematological Malignancy and Solid Tumor Patients, Brazilian Journal of Microbiology (2023)
11. Candida Africana and Candida Dubliniensis As Causes of Pediatric Candiduria: A Study Using Hwp1 Gene Size Polymorphism, AIMS Microbiology (2020)
12. Antifungal Activity of Anethum Graveolens Extract and Atrovastatin Against Candida Species Compared to Fluconazole, Journal of Isfahan Medical School (2015)
13. Evaluation of Prevalence of New Candida Species (C. Orthopsilosis and C. Metapsilosis) Among C. Parapsilosis Group Isolated From Patients by Pcr-Rflp of Sadh Gene in Iran, Journal of Isfahan Medical School (2011)
14. Candidemia in Children Caused by Uncommon Species of Candida, Archives of Pediatric Infectious Diseases (2018)
15. Identification and Frequency of Candida Species in Patients With Different Forms of Candidiasis in Isfahan, Using Pcr-Rflp Method, Journal of Isfahan Medical School (2011)
16. In Vitro Activity of Two Nanoparticles on Clinical Isolates of Candida Parapsilosis, Showing Resistance Against Antifungal Agents in Children, Archives of Clinical Infectious Diseases (2017)
17. The Evaluation of the Overexpression of the Erg-11, Mdr-1, Cdr-1, and Cdr-2 Genes in Fluconazole-Resistant Candida Albicans Isolated From Ahvazian Cancer Patients With Oral Candidiasis, Journal of Clinical Laboratory Analysis (2022)
18. Low Level of Antifungal Resistance in Iranian Isolates of Candida Glabrata Recovered From Blood Samples in a Multicenter Study From 2015 to 2018 and Potential Prognostic Values of Genotyping and Sequencing of Pdr1, Antimicrobial Agents and Chemotherapy (2019)
19. Amino Acid Substitutions in Erg11p of Azole-Resistant Candida Glabrata: Possible Effective Substitutions and Homology Modelling, Journal of Global Antimicrobial Resistance (2016)
20. The Frequency of Oral Candidiasis in Children and Adolescents With Leukemia in Isfahan Province, Iran, Journal of Isfahan Medical School (2017)
21. Prevalence, Molecular Identification, and Genotyping of Candida Species Recovered From Oral Cavity Among Patients With Diabetes Mellitus From Tehran, Iran, Advanced Biomedical Research (2022)
22. Identification of Candida Species Isolated From Candiduria Patients Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism in Isfahan, Iran, Journal of Isfahan Medical School (2016)
23. Epidemiology and Antifungal Susceptibility of Candida Species Isolated From 10 Tertiary Care Hospitals in Iran, Microbiology Spectrum (2022)
24. Phospholipase Activity of Candida Species Isolated From Diabetic Patients, Advanced Biomedical Research (2023)
25. Rapid Differential Diagnosis of Vaginal Infections Using Gold Nanoparticles Coated With Specific Antibodies, Medical Microbiology and Immunology (2019)
26. Utilization of Size Polymorphism in Its1 and Its2 Regions for Identification of Pathogenic Yeast Species, Journal of Medical Microbiology (2017)
27. Caspofungin-Non-Susceptible Candida Orthopsilosis Isolated From Onychomycosis in Iran, Iranian Journal of Public Health (2017)
28. Frequency of Uncommon Clinical Yeast Species Confirmed by Its-Sequencing, Archives of Clinical Infectious Diseases (2019)
29. Molecular Characterization of Candida Dubliniensis and Candida Albicans in the Oral Cavity of Drug Abusers Using Duplex Polymerase Chain Reaction, Current Medical Mycology (2018)
30. Microbial Epidemiology of Candidaemia in Neonatal and Paediatric Intensive Care Units at the Children's Medical Center, Tehran, Mycoses (2018)
31. Candida Palmioleophila Candidemia and Bacterial Co-Infection in a 3-Month-Old Infant With Biliary Atresia, Frontiers in Cellular and Infection Microbiology (2023)
32. Multicenter Identification and Antifungal Susceptibility Patterns of Candida Species Isolated From Clinical Samples, Jundishapur Journal of Microbiology (2017)
33. The Frequency of Candiduria in Hospitalized Patients at Nephrology Department, Labbafinejad Hospital, Tehran, Iran, Journal of Isfahan Medical School (2017)
34. Non-Invasive Methods to Diagnose Fungal Infections in Pediatric Patients With Hematologic Disorders, Jundishapur Journal of Microbiology (2016)
35. Evaluation of the Therapeutic Effect of 15 Ppm Silver Nanoparticle Spray Compared to Clotrimazole 1% on Candida Vaginitis: A Randomized Controlled Clinical Trial, Journal of Family and Reproductive Health (2023)
36. Identification of Candida Species and Antifungal Susceptibility in Cancer Patients With Oral Lesions in Ahvaz, Southern West of Iran, Advanced Biomedical Research (2020)
37. In Vitro Antifungal Susceptibility Pattern of Candida Species Isolated From Gastroesophageal Candidiasis, Gastroenterology and Hepatology from Bed to Bench (2021)
38. Yeast Species in the Respiratory Samples of Covid-19 Patients; Molecular Tracking of Candida Auris, Frontiers in Cellular and Infection Microbiology (2024)
39. Candida Infections Among Neutropenic Patients, Caspian Journal of Internal Medicine (2016)
40. Antifungal Susceptibility Testing of Candida Species Isolated From the Immunocompromised Patients Admitted to Ten University Hospitals in Iran: Comparison of Colonizing and Infecting Isolates, BMC Infectious Diseases (2017)
41. Frequency of Candida Species Isolated From Patients in Children’S Medical Center, Tehran, Iran, Archives of Pediatric Infectious Diseases (2018)
42. Species Distribution and Antifungal Susceptibility Profile of Candida Isolates From Blood and Other Normally Sterile Foci From Pediatric Icu Patients in Tehran, Iran, Medical Mycology (2020)
43. Frequency of Candida Species in the Oral Cavity of Narcotics and Stimulants Smokers in Isfahan, Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method, Advanced Biomedical Research (2020)
44. Epidemiology, Species Distribution, Antifungal Susceptibility, and Outcome of Candidemia in Intensive Care Units in Isfahan, Iran, Current Medical Mycology (2022)
45. Comparison of Proteinase Activity, Hemolysin Production, and Adherence Ability of Candida Albicans Isolates Obtained From Gastroesophageal Lesions and Urinary Tract Infections, Journal of Research in Medical Sciences (2022)
46. Molecular Characterization and Antifungal Susceptibilitytesting of Candida Nivariensis From Blood Samples – an Iranian Multicentre Study and a Review of the Literature, Journal of Medical Microbiology (2019)
47. Identification of Candida Species in Oral Cavity of Smokers and Nonsmokers, Journal of Isfahan Medical School (2016)
48. Translation Elongation Factor 1-Alpha Gene As a Marker for Diagnosing of Candidal Onychomycosis, Current Medical Mycology (2020)
49. Detection of Candida Albicans in Oral Squamous Cell Carcinoma by Fluorescence Staining Technique, Dental Research Journal (2015)