In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions



Masoumeh SH^{1, 5} ; Mosayyeb NA² ; Nasri H³ ; Hadi M⁴ ; Mahmoud RK^{1, 5} Show Affiliations
Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Ampeloprasum is traditionally used for gastrointestinal disorders. In this experimental study, the effect of ethanolic extract of Allium ampeloprasum was investigated on the rat ileum contractions. The mechanisms were also evaluated. Methods: In this experimental study, 48 male Wistar rats were randomly designated into six equal groups, including control and cumulative concentrations of Allium ampeloprasum, propranolol, naloxone, L-name, and CaCl2. In the day of the experiment, the ileum was dissected and its contractions were recorded in Tyrode solution in an organ bath. To evaluate the mechanisms involved, propranolol, naloxone, or L-name was incubated and the percent of changes in contractions were recorded. Findings: The extract of Allium ampeloprasum dose-dependently reduced the contractions induced by KCl (60 mM) (P < 0.0001); propranolol reversed this effect (P < 0.0010), but L-name and naloxone were not effective. CaCl2 increased the contractions (P < 0.0010) and the extract reversed this effect. Conclusion: Allium ampeloprasum leaves can affect the ileum contractions through beta adrenoreceptors and voltage-dependent calcium channels and might be used in gastrointestinal disorders.