Crocin Effects on Human Myeloma Cells Regarding Intracellular Redox State, Dna Fragmentation, and Apoptosis or Necrosis Profile Publisher



Rezaee R¹ ; Jamialahmadi K² ; Zanjani BR³ ; Mahmoudi M⁴ ; Abnous K^{5, 6} ; Rabe SZT⁴ ; Tabasi N⁴ ; Zali M¹ ; Rezaee M⁷ ; Amin B⁸ ; Karimi G¹ Show Affiliations
Source: Jundishapur Journal of Natural Pharmaceutical Products Published:2014

Abstract

Background: Well-documented studies reported several pharmacological properties for crocin, the active compound of Crocus sativus, such as its antitumor, radical scavenging, antidepressant, and memory-enhancing effects. Objectives: We aimed to evaluate the possible cytotoxic activity of crocin on B lymphocytes in human myeloma (U266 cell line) after 24- and 48-hour treatment. Materials and Methods: For this purpose, cell viability was determined by the colorimetric MTT assay and cell death pattern was evaluated using Annexin V-FITC/propidium iodide (PI) apoptosis detection kit. ROS (reactive oxygen species) production and DNA fragmentation were assessed using 2′,7′-dichlorofluorescein diacetate (DCFH-DA) kit and PI staining, respectively. Results: The highest concentration of crocin significantly decreased ROS production after 48 hours of treatment. However, crocin had no effect on the expression level of HSP (Heat shock protein). Additionally, its administration caused a mild decline in cell viability and a mild increase in the population of DNA fragmented cells as well as apoptosis. Conclusions: In our study, no prominent effect was seen; therefore, in order to have a better perspective of crocin activity against cancerous cell lines, further studies are highly recommended. © 2014, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences.