Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Lncarod Promotes the Proliferation and Migration of Gastric Cancer: A Bioinformatics Analysis and Experimental Validation Publisher Pubmed



Ansari S1 ; Nikpour P1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Hezar Jarib Street, Isfahan, Iran

Source: Functional and Integrative Genomics Published:2023


Abstract

Gastric cancer (GC) is a heterogeneous disease at the molecular and clinical levels. The diffuse subtype is associated with more aggressive behavior and poor prognosis than the intestinal subtype. Epithelial-to-mesenchymal transition (EMT) may be involved in the diffuse mesenchymal phenotype. Long non-coding RNA (lncRNA) deregulation plays a vital role in GC development and progression. Here, we aimed to comprehensively disclose lncRNAs associated with GC diffuse/mesenchymal type. RNA-sequencing expression profiles of patients with stomach adenocarcinoma and the corresponding clinical data were downloaded from The Cancer Genome Atlas database. Differentially expressed lncRNAs related to tumor samples and diffuse subtype were identified. The lncRNA activating regulator of DKK1 (LNCAROD) was experimentally studied. Furthermore, a lncRNA-miRNA-mRNA network was constructed to identify potential biological functions of LNCAROD. LNCAROD expression was detected by reverse transcription-quantitative polymerase chain reaction in GC cell lines. LNCAROD expression was silenced using the small interference RNA strategy. Cell proliferation and migration were evaluated using colony formation assay, scratch wound healing, and Transwell migration assays. LNCAROD was significantly upregulated in some GC cells. The knocking down of LNCAROD significantly reduced cell proliferation and migration. LNCAROD-miR-181-PROX1 axis was introduced as a potential regulatory mechanism by which LNCAROD may exert its functions in cells. Our findings highlight that LNCAROD is involved in cell proliferation and migration in GC and supports its implicit role in regulating EMT. It may serve as a potential diagnostic and therapeutic target in GC. In addition, LNCAROD may function through the possible regulatory axis in GC development. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Related Docs
View other Related Docs
1. Integrated Analysis of Transcriptome and Epigenome Reveals Ensr00000272060 As a Potential Biomarker in Gastric Cancer, Epigenomics (2024)
2. Construction of a Three-Component Regulatory Network of Transcribed Ultraconserved Regions for the Identification of Prognostic Biomarkers in Gastric Cancer, Journal of Cellular Biochemistry (2023)
3. Long Non-Coding Rnas As Potential Biomarkers or Therapeutic Targets in Gastric Cancer, Gastroenterology and Hepatology from Bed to Bench (2023)
4. Long Noncoding Rnas in Gastric Cancer Carcinogenesis and Metastasis, Briefings in Functional Genomics (2017)
5. The Pathogenic Roles of Lncrna-Taurine Upregulated 1 (Tug1) in Colorectal Cancer, Cancer Cell International (2022)
6. A Comprehensive Review of the Role of Lncrnas in Gastric Cancer (Gc) Pathogenesis, Immune Regulation, and Their Clinical Applications, Pathology Research and Practice (2023)
7. Identification of Cancer/Testis Antigens Related to Gastric Cancer Prognosis Based on Co-Expression Network and Integrated Transcriptome Analyses, Advanced Biomedical Research (2023)
8. Introduction of Lpin1 As a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer Via Integrative Bioinformatics Analysis of a Competing Endogenous Rna Network and Experimental Validation, Iranian Journal of Basic Medical Sciences (2024)
Experts (# of related papers)
View all Related Experts
Parvaneh Nikpour (17)
Mohammadreza Sharifi (13)
Other Related Docs
9. Aberrant Expression of Plncrna-1 and Tug1: Potential Biomarkers for Gastric Cancer Diagnosis and Clinically Monitoring Cancer Progression, Biomarkers in Medicine (2017)
10. Investigating Combined Hypoxia and Stemness Indices for Prognostic Transcripts in Gastric Cancer: Machine Learning and Network Analysis Approaches, Biochemistry and Biophysics Reports (2025)
11. Steroid Receptor Rna Activator Gene Footprint in the Progression and Drug Resistance of Colorectal Cancer Through Oxidative Phosphorylation Pathway, Life Sciences (2021)
12. Dysregulated of the Fam138e and Clrn1-As1 Lncrnas Could Be Diagnosis Biomarkers in Colorectal Cancer, Human Gene (2024)
13. Lncrna–Mirna–Mrna Networks of Gastrointestinal Cancers Representing Common and Specific Lncrnas and Mrnas, Frontiers in Genetics (2022)
14. Comprehensive Analysis of Ernas and Genes Involved in Gastric Cancer and Constructing a Prognostic Model Predicting the Overall Survival of Patients, Journal of Isfahan Medical School (2023)
15. Emerging Circulating Mirnas and Lncrnas in Upper Gastrointestinal Cancers, Expert Review of Molecular Diagnostics (2020)
16. Analysis of a Four-Component Competing Endogenous Rna Network Reveals Potential Biomarkers in Gastric Cancer: An Integrated Systems Biology and Experimental Investigation, Advanced Biomedical Research (2023)
17. Mir-146A Is Deregulated in Gastric Cancer, Journal of Cancer Research and Therapeutics (2019)
18. Micrornas Regulating Wnt Signaling Pathway in Colorectal Cancer: Biological Implications and Clinical Potentials, Functional and Integrative Genomics (2022)
19. Functional Roles of Lncrna-Tug1 in Hepatocellular Carcinoma, Life Sciences (2022)
20. The Effect of Long Non-Coding Rna Pvt1 Inhibition by Antisense Lna Gapmers Technology on Proliferation of Human Acute Erythroleukemia Cells, Journal of Isfahan Medical School (2018)
21. A Bioinformatics Approach of Specificity Protein Transcription Factors in Head and Neck Squamous Cell Carcinoma, Research in Pharmaceutical Sciences (2024)
22. Novel Somatic Variants in Ctnna1 Gene in Iranian Patients With Diffuse Gastric Cancer, Gastroenterology and Hepatology from Bed to Bench (2021)
23. Lna Inhibitor in Microrna Mir-23B As a Potential Anti-Proliferative Option in Human Hepatocellular Carcinoma, Journal of Gastrointestinal Cancer (2020)
24. Microrna Binding Site Polymorphism in Inflammatory Genes Associated With Colorectal Cancer: Literature Review and Bioinformatics Analysis, Cancer Gene Therapy (2020)
25. Inducing Cell Proliferative Prevention in Human Acute Promyelocytic Leukemia by Mir-182 Inhibition Through Modulation of Casp9 Expression, Biomedicine and Pharmacotherapy (2017)
26. A Prediction of the Crnde Role by Modulating Nf-Κb Pathway in Inflammatory Bowel Disease (Ibd), Biochemistry and Biophysics Reports (2024)
27. Reduce Proliferation of Human Bone Marrow Cells From Acute Myeloblastic Leukemia With Minimally Differentiation by Blocking Lncrna Pvt1, Clinical and Translational Oncology (2020)
28. Non-Coding Rnas and Glioblastoma: Insight Into Their Roles in Metastasis, Molecular Therapy Oncolytics (2022)
29. A H. Pylori-Independent Mir-21 Overexpression in Gastric Cancer Patients, Gene Reports (2019)
30. Methylation and Polymorphism in Cdh1 Gene Promoter Among Patients With Diffuse Gastric Cancer, International Journal of Preventive Medicine (2022)
31. Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes, Diagnostics (2022)
32. Msi1 Overexpression in Diffuse Type of Gastric Cancer, Pathology Research and Practice (2013)
33. Molecular Mechanisms of Mir-1236 in the Assessment of Tumor Lymphangiogenesis in Human Ovarian Cancer Patients, Journal of Gene Medicine (2023)
34. Mir-1236: Key Controller of Tumor Development and Progression: Focus on the Biological Functions and Molecular Mechanisms, Pathology Research and Practice (2023)
35. Inhibition of Microrna Mir-92A Induces Apoptosis and Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia Through Modulation of P63 Expression, Molecular Biology Reports (2014)
36. Gastrointestinal Cancers, Cancer Genetics and Psychotherapy (2017)
37. Exploring Potential Roles of Long Non-Coding Rnas in Cancer Immunotherapy: A Comprehensive Review, Frontiers in Immunology (2024)
38. A Computational Framework to Infer Prostate Cancer-Associated Long Noncoding Rnas and Analyses for Identifying a Competing Endogenous Rna Network, Genetic Testing and Molecular Biomarkers (2021)
39. Mir-17-92 Host Gene, Uderexpressed in Gastric Cancer and Its Expression Was Negatively Correlated With the Metastasis, Indian Journal of Cancer (2015)
40. Degradation of Hox Transcript Antisense Rna Provoked Apoptosis and Necrosis in Human Ovarian Cancer Cells, Indian Journal of Gynecologic Oncology (2020)
41. Epigenetic Regulation of Autophagy in Gastrointestinal Cancers, Biochimica et Biophysica Acta - Molecular Basis of Disease (2022)
42. Rs12904 Polymorphism in the 3'-Untranslated Region of Ephrin A1 Ligand and the Risk of Sporadic Colorectal Cancer in the Iranian Population, Journal of Cancer Research and Therapeutics (2019)
43. A Comparative Study of Hotair Expression in Breast Cancer Patient Tissues and Cell Lines, Cell Journal (2019)
44. Interplays Between Non-Coding Rnas and Chemokines in Digestive System Cancers, Biomedicine and Pharmacotherapy (2022)
45. Down-Regulation of Circular Rna Itch and Circhipk3 in Gastric Cancer Tissues, Turkish Journal of Medical Sciences (2019)
46. Cross-Talk Between Oxidative Stress Signaling and Microrna Regulatory Systems in Carcinogenesis: Focused on Gastrointestinal Cancers, Biomedicine and Pharmacotherapy (2020)
47. Crispr Technology: A Versatile Tool to Model, Screen, and Reverse Drug Resistance in Cancer, European Journal of Cell Biology (2023)
48. Induction of Apoptosis and Necrosis in Human Acute Erythroleukemia Cells by Inhibition of 47Tlong Non-Coding Rna Pvt1, Molecular Biology Research Communications (2018)
49. Construction of a Cell Cycle-Specific Lncrna-Mirna-Mrna Network Reveals Novel Key Lncrnas in Colorectal Cancer, Biomarkers (2024)
50. Novel Biomarkers for Neoplastic Progression From Ulcerative Colitis to Colorectal Cancer: A Systems Biology Approach, Scientific Reports (2023)