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Dysregulated of the Fam138e and Clrn1-As1 Lncrnas Could Be Diagnosis Biomarkers in Colorectal Cancer Publisher



Karimi Z1 ; Asghari Moghaddam N1 ; Yousefi M2, 6 ; Abdolvand M3, 4, 5 ; Zamani A6 ; Fatahi Dolatabadi N6, 7 ; Peymani M8
Authors
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Authors Affiliations
  1. 1. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  3. 3. Cellular, Molecular, and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
  6. 6. Gene Raz Bu Ali, Genetic and Biotechnology Academy, Isfahan, Iran
  7. 7. Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran
  8. 8. Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Source: Human Gene Published:2024


Abstract

Background: Colorectal cancer (CRC) is a malignant tumor that is prone to metastasis and seriously affects human health and longevity. Therefore, identifying suitable biomarkers for diagnostic and therapeutic purposes in CRC is a pressing requirement. The development of bioinformatics analysis has led to the introduction of an increasing number of long non-coding RNAs (lncRNAs) as potential biomarkers. Methods: The Cancer Genome Atlas Program (TCGA) data for CRC were computationally analyzed to identify new and potential lncRNAs that are dysregulated in CRC. Real-time qPCR was conducted to quantitatively measure the expression levels of FAM138E and CLRN1-AS1 lncRNAs. Result: This approach led us to select FAM138E and CLRN1-AS1 lncRNAs as dysregulated lncRNAs in the TCGA database. We then quantified the expression levels of these lncRNAs, which interestingly showed overexpression of CLRN1-AS1 (P-value<0.0001, Fold change = 1.55) and downregulation of FAM138E (P-value = 0.012, Fold change = 0.028) in tumors compared to normal adjacent tissues. Conclusion: The evaluated associations with prognosis in CRC using data from TCGA database showed that these lncRNAs could be used as diagnostic factors to detect tumors from normal samples. These findings may have implications for the deeper characterization of FAM138E and CLRN1-AS1 lncRNAs in colorectal cancer. © 2023 Elsevier B.V.
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