Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Can Zeta Sperm Selection Method, Recover Sperm With Higher Dna Integrity Compare to Density Gradient Centrifugation?



Khajavi NA1 ; Razavi S1 ; Mardani M1 ; Tavalaee M2 ; Deemeh MR3 ; Nasresfahani MH2, 3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Andrology and Embryology, Reproductive Medicine Research Center, Royan Institute, Tehran, Iran
  3. 3. Isfahan Fertility and Infertility Center, Isfahan, Iran

Source: Iranian Journal of Reproductive Medicine Published:2009

Abstract

Background: Sperm selection for ICSI based on morphology and motility might not be relevant to chromatin integrity. Thus sperm selection based on sperm characteristics has been suggested. Objective: The aim of this study was to compare the efficiency of Zeta method with routine Density Gradient Centrifugation method (DGC) for the selection of sperm with higher DNA integrity. Materials and Methods: Semen samples were obtained from 63 individuals referring to Andrology Unit of Isfahan Fertility and Infertility Center. Semen analysis was carried out according to WHO criteria. Each semen sample was divided into three equal portions. One portion was used as control, the second portion was used for Zeta method and the third portion underwent DGC method. Each portion was evaluated to DNA integrity by TUNEL assay. Student t-test was carried out using SPSS and p-value lower than 0.05 was considered significant. Results: The mean number of sperm DNA fragmentation in Zeta and DGC methods were significantly decreased compare to the control group (p<0.001). In addition, Zeta method was more efficient than the DGC method in the selection of sperm with intact DNA (p<0.001). Conclusion: The Zeta method appears to be a suitable procedure to recover sperm with normal DNA integrity.
Related Docs
View other Related Docs
1. Selection of Sperm Based on Combined Density Gradient and Zeta Method May Improve Icsi Outcome, Human Reproduction (2009)
2. The Comparison of Efficiency of Density Gradient Centrifugation and Zeta Methods in Separation of Mature Sperm With Normal Chromatin Structure, Yakhteh (2009)
3. Evaluation of Zeta and Ha-Binding Methods for Selection of Spermatozoa With Normal Morphology, Protamine Content and Dna Integrity, Andrologia (2010)
4. Evaluation of Sperm Selection Procedure Based on Hyaluronic Acid Binding Ability on Icsi Outcome, Journal of Assisted Reproduction and Genetics (2008)
5. Can Altered Expression of Hspa2 in Varicocele Patients Lead to Abnormal Spermatogenesis?, International Journal of Fertility and Sterility (2010)
6. Efficiency of Sil-Select and Percoll to Recover Spermatozoa With Normal Chromatin and Morphology and the Effect of These Parameters on Fertilization, Embryo Quality and Cleavage Score, Middle East Fertility Society Journal (2003)
7. Effect of Human Sperm Chromatin Anomalies on Fertilization Outcome Post-Icsi, Andrologia (2003)
8. The Role of Sperm Chromatin Anomalies on the Outcome of Assisted Reproductive Techniques, Yakhteh (2006)
Experts (# of related papers)
View all Related Experts
Shahnaz Razavi (30)
Mohammad Mardani (15)
Other Related Docs
9. Sperm Dna Damage and Its Relation With Leukocyte Dna Damage, Reproductive Toxicology (2010)
10. Relationship Between Protamine Deficiency With Fertilization Rate and Incidence of Sperm Premature Chromosomal Condensation Post-Icsi, Andrologia (2004)
11. Evaluation of Sperm Selection Procedure Based on Its Binding Ability to Hyaluronic Acid on Icsi Outcome, Journal of Isfahan Medical School (2008)
12. Relation Between Different Human Sperm Nuclear Maturity Tests and in Vitro Fertilization, Journal of Assisted Reproduction and Genetics (2001)
13. Failed Fertilization After Icsi and Spermiogenic Defects, Fertility and Sterility (2008)
14. Credibility of Chromomycin A3 Staining in Prediction of Fertility, International Journal of Fertility and Sterility (2009)
15. Influence of Sperm Chromatin Anomalies on Assisted Reproductive Technology Outcome, Fertility and Sterility (2009)
16. Effect of Varicocelectomy on Semen Parameters and Protamine Deficiency 3 and 6 Months Post Surgery, Journal of Isfahan Medical School (2007)
17. Effect of Saffron on Rat Sperm Chromatin Integrity, Iranian Journal of Reproductive Medicine (2014)
18. Effects of Failed Oocyte Activation and Sperm Protamine Deficiency on Fertilization Post-Icsi, Reproductive BioMedicine Online (2007)
19. Relationship Between Sperm Parameters and Intracytoplasmic Sperm Injection Outcome, Middle East Fertility Society Journal (2015)
20. Can Sperm Protamine Deficiency Induce Sperm Premature Chromosomal Condensation?, Andrologia (2006)
21. Sperm Mtt Viability Assay: A New Method for Evaluation of Human Sperm Viability, Journal of Assisted Reproduction and Genetics (2002)
22. Comparing the Roles of Sperm Chromatin Integrity and Apoptosis in Intrauterine Insemination Outcomes of Couples With Mild Male and Female Factor Infertility, Journal of Reproduction and Infertility (2014)
23. Effect of Protamine-2 Deficiency on Icsi Outcome, Reproductive BioMedicine Online (2004)
24. Effect of Human Sperm Mtt Viability Test on Outcome of Intracytoplasmic Sperm Injection, Yakhteh (2006)
25. Optimizing Acridine Orange Staining for Assessment of Protective Effects of Saffron and Vitamin E on Rat Sperm Dna Structure, Journal of Isfahan Medical School (2014)
26. Effect of Varicocelectomy on Semen Parameters and Sperm Chromatin Status, Yakhteh (2005)
27. Effect of Polyvinyl Pyrrolidone on Sperm Membrane Integrity and Chromatin Status, International Journal of Fertility and Sterility (2008)
28. Effect of Chromatin Condensation on Frozen-Thawed Sperm Dna Integrity in Normozoospermic Men, Scientific Journal of Kurdistan University of Medical Sciences (2019)
29. Protective Effects of Zinc on Rat Sperm Chromatin Integrity Involvement: Dna Methylation, Dna Fragmentation, Ubiquitination and Protamination After Bleomycin Etoposide and Cis-Platin Treatment, Theriogenology (2020)
30. Treatment Outcome Following Intracytoplasmic Injection of Sperm Retrieved From Ejaculate, Epididymis, or Testis of Infertile Men, Archives of Iranian Medicine (2004)
31. Varicocelectomy: Semen Parameters and Protamine Deficiency, International Journal of Andrology (2009)
32. Effects of Main Semen Parameters on Intrauterian Insemination (Iui) Outcome, Journal of Isfahan Medical School (2010)
33. The Correlation Between Sperm Dna Fragmentation and the Expression of Long Non-Coding Rna Hotair in Infertile Men, Journal of Isfahan Medical School (2021)
34. A Survey on Main Semen Parameters in Natural Pregnancy and Intrauterine Insemination: Are There Any Significant Differences?, American Journal of Men's Health (2018)
35. Does Folate Deficiency Alter Sperm Parameters in Infertile Males?, Immunopathologia Persa (2024)
36. The Effects of Main Semen Parameters on Intra-Uterine Insemination (Iui) Outcome, Journal of Isfahan Medical School (2013)
37. Assessment of the Changing Trend of Different Kinds of Sperm Motility During the Storage of Normozoospermic Men’S Semen Samples at 4-6 ºc, Journal of Isfahan Medical School (2017)
38. Associations of Major Dietary Patterns and Dietary Diversity Score With Semen Parameters: A Cross-Sectional Study in Iranian Infertile Men, International Journal of Fertility and Sterility (2020)
39. The Relationship of Oocyte and Sperm Membrane Binding Protein With the Success of Intrauterine Insemination, Journal of Isfahan Medical School (2014)
40. Role of Dietary Antioxidants and Vitamins Intake in Semen Quality Parameters: A Cross-Sectional Study, Clinical Nutrition ESPEN (2022)
41. Investigation of Association Between Genetic Diversities in Yq11.223 Region With Men Infertility in Isfahan Population, Journal of Isfahan Medical School (2011)
42. Effect of Zinc on Spermatogenesis and Sperm Chromatin Condensation in Bleomycin, Etoposide, Cisplatin Treated Rats, Cell Journal (2019)
43. Gonadotropins in Infertile Men With Idiopathic Hypogonadotropic Hypogonadism, International Journal of Fertility and Sterility (2008)
44. The Association Between Healthy Eating Index Score With Semen Parameters in Infertile Men: A Cross-Sectional Study, International Journal of Reproductive BioMedicine (2022)
45. Effects of Low Doses of Exogenous Thymoquinone on Sperm Motility and Viability of Normozoospermic Men, Journal of Isfahan Medical School (2014)
46. Investigating the Correlation Between Ubiquitination With Motility, Morphology, and Dna Methylation in Rat Sperm, Journal of Isfahan Medical School (2017)
47. The Effect of Consecutive Ejaculation on the Sperm Parameters in the Oligo-Astheno-Teratozoospermia (Oat) Men, Zygote (2025)
48. Is There a Relationship Between Serum Vitamin D and Semen Parameters? a Cross-Sectional Sample of the Iranian Infertile Men, Basic and Clinical Andrology (2021)
49. The Relationship Between Plant and Animal Based Protein With Semen Parameters: A Cross-Sectional Study in Infertile Men, Clinical Nutrition ESPEN (2022)
50. Correlation of Obesity and Serum Vitamin D Levels With Sperm Dna Integrity, Sperm Quality, and Sperm Viability in Normozoospermia Men, Advanced Biomedical Research (2022)