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Efficiency of Sil-Select and Percoll to Recover Spermatozoa With Normal Chromatin and Morphology and the Effect of These Parameters on Fertilization, Embryo Quality and Cleavage Score



Nasresfahani MH1, 2, 3 ; Razavi S3 ; Mardani M3 ; Tofighhesabi S3
Authors
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Authors Affiliations
  1. 1. Royan Institute, Department of Clinical Embryology, Tehran, Iran
  2. 2. Isfahan Fertil./Infertility Center, Isfahan, Iran
  3. 3. Department of Anatomy, Isfahan Univ. of Medical Sciences, Isfahan, Iran

Source: Middle East Fertility Society Journal Published:2003

Abstract

Objective: To evaluate and compare efficiency of Sil-Select with Percoll to recover sperms with normal chromatin or morphology and also to study the effect of these parameters on fertilization, embryo quality and cleavage score. Materials and methods: Assessment of semen parameters, protamine deficiency (CMA3) and excessive histone (aniline blue) were carried out before and after sperm processing on 46 semen samples from IVF patients referring to Isfahan fertility and infertility center. Results: Both Sil-select and Percoll were efficient to reduce sperm chromatin and morphology anomalies. Significant correlation, were obtained between sperm morphology (strict criteria), protamine deficiency and excessive histone with in vitro fertilization rate. Among these parameters only sperm morphology showed a significant correlation with embryo quality and cleavage score. Conclusion: Sil-Select is a suitable alternative for Percoll to recover sperm with normal chromatin and morphology. Furthermore density gradient techniques are recommended for sperm processing for IVF and especially for ICSI.
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