Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Comparing the Perimplantation Thickness of Mouse Uterine Endometrium in Natural Cycle and Those Received Human Menopausal Gonadotropin- Human Chorionic Gonadotropin (Hmg–Hcg) and Sildenafil Citrate



Rashidi B1 ; Mardani M1 ; Peyvandikarizbodagh M1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Uterine endometrium plays an important role in the implantation process. This layer of the uterus is affected during the menstrual period. Due to the influence of inhibitors and stimulators on uterine endometrium, the aim of this study was to assess the changes in endometrial thickness after injections of sildenafil citrate in super-ovulated mice. Methods: Thirty adult female mice were divided into 3 groups of control, gonadotropin and gonadotropin + sildenafil citrate. In 2 experimental groups, the mice received 7.5 IU of human menopausal gonadotropin (HMG) and later human chorionic gonadotropin (HCG). Then, every two female mice with one male mouse were put in one cage for fertilization. In gonadotropin + sildenafil citrate group, 3 mg/kg sildenafil citrate administrated at 24, 48, and 72 hours after HMG injection. Ninety six hours after HMG injection, the mice in 3 groups were sacrificed and their uterine specimens were prepared for light microscopic studies. Findings: The mean ± SD endometrial thickness was 358.05 ± 13.35 μm in control, 389.84 ± 12.46 μm in gonadotropin and 316.81 ± 12.10 μm in gonadotropin + sildenafil citrate groups. The endometrial thickness in all groups were not significantly deferent from each other (P < 0.05 for all). Conclusion: Using ovarian stimulation, followed by sildenafil citrate injection, would not modify the endometrial thickness of mouse uterus. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. Evaluation of the Endometrial Thickness in Mice After Ovarian Stimulation and Using Progesterone, Journal of Isfahan Medical School (2014)
2. Comparison of Morphological and Morphometrical Characteristics in the Glandular Epithelium of Mouse Endometrium in Preimplantation Period After Administration Hmg-Hcg, Progesterone and Sildenafil Citrate, Journal of Isfahan Medical School (2010)
3. Ultrastructural and Morphometrical Study of Preimplantation Endometrium in Superovulated Mice Treated With Progesterone or Sildenafil, International Journal of Reproductive BioMedicine (2013)
4. The Survey of Angiogenesis and Myometrial Thickness of Preimplantation Mouse Uterine in Natural Cycle in Those Received Human Menopausal Gonadotropin-Human Chorionic Gonadotropin (Hmg–Hcg) and Sildenafil Citrate, Journal of Isfahan Medical School (2017)
5. Evaluation of Pinopodes Expression on the Mouse Endometrium Immediately Before Implantation by Treatment With Hmg/Hcg and Sildenafil Citrate Administration, Iranian Journal of Basic Medical Sciences (2012)
6. Implantation Window and Angiogenesis, Journal of Cellular Biochemistry (2017)
7. Progesterone and Ovarian Stimulation Control Endometrial Pinopode Expression Before Implantation in Mice, Pathophysiology (2012)
8. The Effects of Different Luteal Support Hormones on Endometrial Alkaline Phosphatase Activity and Endometrial Thickness in Superovulated Mice, Iranian Journal of Reproductive Medicine (2010)
Experts (# of related papers)
View all Related Experts
Bahman Rashidi (12)
Parvaneh Nikpour (5)
Other Related Docs
9. Effect of Granulocyte Colony Stimulating Factor (G-Csf) on Ivf Outcomes in Infertile Women: An Rct, International Journal of Reproductive BioMedicine (2016)
10. The Laparoscopic Ovarian Electrocautery Versus Gonadotropin Therapy in Infertile Women With Clomiphene Citrate-Resistant Polycystic Ovary Syndrome; a Randomized Controlled Trial., JPMA. The Journal of the Pakistan Medical Association (2012)
11. Evaluation of Diabetes Effects on the Expression of Leukemia Inhibitory Factor and Vascular Endothelial Growth Factor a Genes and Proteins at the Time of Endometrial Receptivity After Superovulation in Rat Model, Advanced Biomedical Research (2019)
12. Ovarian Stimulation and Exogenous Progesterone Affect the Endometrial Mir-16-5P, Vegf Protein Expression, and Angiogenesis, Microvascular Research (2021)
13. Evaluation of Muc1 Gene Expression at the Time of Implantation in Diabetic Rat Models Treated With Insulin, Metformin and Pioglitazone in the Normal Cycle and Ovulation Induction Cycle, International Journal of Fertility and Sterility (2020)
14. Effects of L-Arginine and L-Nitro-Arginine Methyl Ester (L-Name) in the First Half of Pregnancy on Embryos and Offspring Testis Tissue Before and After Birth, Journal of Isfahan Medical School (2016)
15. Evaluation of Vascular Endothelial Growth Factor a and Leukemia Inhibitory Factor Expressions at the Time of Implantation in Diabetic Rats Following Treatment With Metformin and Pioglitazone, International Journal of Reproductive BioMedicine (2020)
16. Role of Granulocyte Colony-Stimulating Factor in Human Reproduction, Journal of Research in Medical Sciences (2018)
17. Can Autologous Platelet Rich Plasma Expand Endometrial Thickness and Improve Pregnancy Rate During Frozen-Thawed Embryo Transfer Cycle? a Randomized Clinical Trial, Taiwanese Journal of Obstetrics and Gynecology (2018)
18. A Study on the Presence of Osteopontin and Α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation, Journal of Reproduction and Infertility (2020)
19. The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture, Journal of Babol University of Medical Sciences (2010)
20. The Effect of Endometrial Cell Culture on Α3 and Β1 Integrin Genes and Protein Expression in Type 2 Diabetic Rats at the Time of Implantation, Cell Journal (2022)
21. Endometrial Pinopode Biomarkers: Molecules and Micrornas, Journal of Cellular Physiology (2018)