Evaluation of the Endometrial Thickness in Mice After Ovarian Stimulation and Using Progesterone



Rashidi B¹ ; Mardani M¹ ; Bodagh MPK¹ Show Affiliations
Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Human Endometrium is a dynamic tissue during the menstrual cycle which can be influenced by ovarian hormones. This layer is the main responsible for the successful implantation of the embryo. One of the most important factors affecting the implantation is endometrial thickness. This study aimed to evaluate the endometrial thickness under the influence of ovulation-stimulating hormones and progesterone. Methods: Thirty adult female mice were divided into 3 groups of control, gonadotropin and gonadotropin + progesterone. In 2 experimental groups, the mice received 7.5 IU human menopausal gonadotropin (HMG) and later, human chorionic gonadotropin (HCG). Then, every two female mice were put in one cage with one male mouse for fertilization. The gonadotropin + progesterone group recieved 1 mg/kg progesterone at the hours 24, 48, and 72 after the injection of human menopausal gonadotropin. Ninety six hours after the injection of human chorionic gonadotropin, the mice in 3 groups were sacrificed and their uterine specimens were prepared for light microscopic studies. Findings: Using the software Motic Image Plus 3.2, endometrial thickness in was 358.06 ± 13.36 μm in control, 389.85 ± 12.46 μm in gonadotropin, and 381.64 ± 13.03 μm in gonadotropin + progesterone group. There were not any significant difference between the control and gonadotropin groups and between the control and gonadotropin + progesterone groups. In addition, the endometrial thickness was not significantly deferent between the 3 groups (P < 0.05 for all of the comparisons). Conclusion: Using ovarian stimulation, followed by progesterone injection, would not modify the endometrial thickness of mouse endometrium. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.