The Effect of Intestinal Microbiota Metabolites on Ht29 Cell Line Using Mtt Method in Patients With Colorectal Cancer Publisher



Jahanisherafat S¹ ; Azimirad M¹ ; Ghasemiansafaei H² ; Amoli HA³ ; Moghim S² ; Sherkat G⁴ ; Zali MR⁵
Source: Gastroenterology and Hepatology from Bed to Bench Published:2019

Abstract

Aim: The aim of this study was to evaluate the effect of intestinal microbiota metabolites in colorectal cancer patients on HT29 cell line using MTT assay. Background: Colorectal cancer is one of the most common malignant tumors. Human guts harbor abundant microbes that adjust many aspects of the host physiology. Increasing studies suggest that gut microbiota play a significant role in the incidence and expansion of CRC, as a result of virulence factors, bacterial metabolites, or inflammatory pathways. Methods: In this cross-sectional study, 60 biopsy samples including 30 cancerous and 30 adjacent healthy tissues were collected from patients with CRC during 2017. Biopsy samples were first cultured on Thioglycollate broth medium for 24hr after which the microbiota metabolites were filtered and stored at -20 C° for further evaluation. HT29 cells were treated by microbiota metabolites at different times (3, 6, 12, 18h) and its viability was assessed by MTT assay. Results: The cells treated with microbiota metabolites showed increased viability and proliferation in time-dependent analysis by MTT assay, but there was not significant differences between the two groups. Conclusion: It seems that microbial metabolites are able to induce proliferation and increase cell viability and thus induce colorectal cancer. ©2019 RIGLD, Research Institute for Gastroenterology and Liver Diseases