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Gut Microbiota and Host Gene Mutations in Colorectal Cancer Patients and Controls of Iranian and Finnish Origin Publisher Pubmed



Sarhadi V1 ; Lahti L2 ; Saberi F3 ; Youssef O1 ; Kokkola A4 ; Karla T5 ; Tikkanen M5 ; Rautelin H6 ; Puolakkainen P5 ; Salehi R3, 7 ; Knuutila S1
Authors
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Authors Affiliations
  1. 1. Department of Pathology, Faculty of Medicine, University of Helsinki, Helsinki, Finland
  2. 2. Department of Future Technologies, University of Turku, Turku, Finland
  3. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. HUCH Gastrointestinal Clinic, University Central Hospital of Helsinki, Helsinki, Finland
  5. 5. Thermo Fisher Scientific, Vantaa, Finland
  6. 6. Department of Medical Sciences, Clinical Microbiology, Uppsala University, Uppsala, Sweden
  7. 7. Pediatric Inherited Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Anticancer Research Published:2020


Abstract

Background/Aim: Gut microbiota plays an important role in colorectal cancer (CRC) and its composition in CRC patients can be influenced by ethnicity and tumour genomics. Herein, the aim was to study the possible associations of ethnicity and gene mutations with the gut microbiota in CRC patients. Materials and Methods: Bacterial composition in stool samples of 83 CRC patients and 60 controls from Iran and Finland was studied by 16S rRNA gene sequencing. The association of gut microbiota composition with CRC, host mutations in KRAS, NRAS and TP53, and ethnicity analysed. Results: Beta diversity analysis indicated significant differences between the Iranian and Finnish gut microbiota composition, in both controls and patients' groups. The Iranian controls had higher abundance of Prevotella and lower abundance of Bacteroides compared to the Finnish controls, while the Finnish patients had higher abundance of Clostridium compared to Iranian patients. Abundance of Ruminococcus was higher in patients compared to the controls. Higher abundances of Herbaspirillum, Catenibacterium and lower abundances of Barnesiella were associated with mutations in NRAS, TP53, and RAS respectively. Conclusion: A possible link of host gene mutations with gut bacterial composition is suggested. © 2020 International Institute of Anticancer Research. All rights reserved.
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