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Immunization Against Leishmania Major Using Uv-Irradiated Promastigote in Experimental Model Balb/C Mice



Rahdar M1, 2 ; Hejazi SH3, 4 ; Askarisabzkohi A2 ; Arjmand R2
Authors
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Authors Affiliations
  1. 1. Infectious and Tropical Diseases Research Center, Health Research Institute Ahvaz, Jundishapur University of Medical Sciences, Ahvaz, Iran
  2. 2. Parasitology Department, Medical School, Jundishapur University of Medical Sciences, Ahvaz, Iran
  3. 3. Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan, Iran
  4. 4. University of Medical Sciences, Isfahan, Iran

Source: Biochemical and Cellular Archives Published:2017

Abstract

Cutaneous leishmaniasis is one of important parasitic disease in many part of the world. Iran is a hyper endemic area with cutaneous leishmaniasis permanently cause by L.major. Many efforts were conducted to achieve reliable vaccine against leishmaniasis by researches. The goal of this study concentrated on induces protective immunity against cutaneous leishmaniasis in experimental animal using UV-C irradiation promastigotes of L.major. Sixteen Balb/c mice divided to two group included vaccination and control group. The number of 2×106 promastigotes of L.major were exposed to more than 400 J/cm of UV-C irradiation and injected to mice with Freund's complete and incomplete adjuvant. After vaccination, all groups injected SC by 2×106 of wild type of L. major promastigotes in stationary phase. The mice examined weekly for lesion until 2 months. The size of lesions were measured weekly and recorded. Five out of 8 (62.5%) in vaccination group didn't show any lesion after 2 months and the size of lesion in other were significantly smaller than control group (P < 0 . 05). Vaccination against cutaneous leishmaniasis by UV irradiated promastigotes is appropriated for future successful control of leishmaniasis.
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