Identification of an Isolate of Pseudomonas Aeroginosa Deposited in Ptcc As a Pha Producer Strain: Comparison of Three Different Bacterial Genomic Dna Extraction Methods Publisher



Sadeghi HMM^{1, 2} ; Najafabadi AJ^{1, 3} ; Abedi D¹ ; Dehkordi AJ¹
Source: Journal of Biological Sciences Published:2008

Abstract

In this study, we used a nucleic acid based method to identify the capability of Polyhydroxy alkanoate (PHA) production of two isolates of Pseudomonas aeroginosa deposited in Persian Type Culture Collection (i.e., Pseudomonas aeroginosa PTCC 1310 and 1740) and present results showed this capability for the PTCC1310 but not for the other isolate. This identified isolate could be used for massive production of the mentioned polymers or its pha locus could be cloned and used for the functional expression of the PHA biopolymers. Here we also compared three bacterial genomic DNA extraction methods with respect to their difficulty, cost and the time of procedure. These methods included simple boiling method, phenol-chloroform method and commercial DNA extraction kit. All three methods gave reasonable amount of DNA that could be used as templates for the detection of pha gene. In conclusion we have identified a Pseudomonas aeroginosa strain present in Iran containing the necessary genes for PHA production and suggest that the simple boiling method can be utilized for further screening of bacterial samples for the presence of these genes. © 2008 Asian Network for Scientific Information.