Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310

Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310 Publisher

Abedi D^{1, 3} ; Najafabadi AJ¹ ; Sadeghi HM¹ ; Vallian S²

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1. Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Isfahan Medical University, Iran
2. Department of Biology, Faculty of Science, Isfahan University, Isfahan, Iran
3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Biotechnology Published:2007

Abstract

Poly (hydroxy alkanoic acid) (PHA) polymers are synthesized by numerous microorganisms. These compounds have similar properties to synthetic plastics with excellent biodegradability. Here we report identification and cloning of phaC1 and phaC2 genes of type II PHA synthase gene from the Iranian isolate of P. aeroginosaPTCC 1310 bacterium. The sequences of both genes were isolated using PCR amplification with specific primers and cloned into pTZ57R cloning vectors as pTZPHAC1 and pTZPHAC2. The correct sequence of the cloned genes was confirmed by restriction digestion followed by partial sequencing. The vectors could be used for future sub-cloning and expression analysis purposes. © 2007 Asian Network for Scientific Information.

2. Optimization of the Expression of Genes Encoding Poly (3-Hydroxyalkanoate) Synthase From Pseudomonas Aeruginosa Ptcc 1310 in Escherichia Coli, Avicenna Journal of Medical Biotechnology (2012)

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Hamid Mirmohammad Sadeghi (2)

Ali Jahanian Najafabadi (2)

Style	Citing Format
MLA	Abedi D, et al.. "Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310." Biotechnology, vol. 6, no. 4, 2007, pp. 497-504.
APA	Abedi D, Najafabadi AJ, Sadeghi HM, Vallian S (2007). Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310. Biotechnology, 6(4), 497-504.
Chicago	Abedi D, Najafabadi AJ, Sadeghi HM, Vallian S. "Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310." Biotechnology 6, no. 4 (2007): 497-504.
Harvard	Abedi D et al. (2007) 'Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310', Biotechnology, 6(4), pp. 497-504.
Vancouver	Abedi D, Najafabadi AJ, Sadeghi HM, Vallian S. Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310. Biotechnology. 2007;6(4):497-504.
BibTex	@article{ author = {Abedi D and Najafabadi AJ and Sadeghi HM and Vallian S}, title = {Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310}, journal = {Biotechnology}, volume = {6}, number = {4}, pages = {497-504}, year = {2007} }
RIS	TY - JOUR AU - Abedi D AU - Najafabadi AJ AU - Sadeghi HM AU - Vallian S TI - Cloning and Partial Sequencing of Phac1 and Phac2 Genes Encoding Poly (3-Hydroxyalkanoate) Synthases From Pseudomonas Aeroginusa Ptcc 1310 JO - Biotechnology VL - 6 IS - 4 SP - 497 EP - 504 PY - 2007 ER -

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