Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Prevalence of Ctx-M Genes in Escherichia Coli Strains in Outpatient and Inpatient Cases With Urinary Tract Infections in Isfahan, Iran



Mobasherizadeh M1 ; Bidoki SK1 ; Mobasherizadeh S2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Genetics and Biotechnology, School of Sciences, Payame Noor University, Tehran, Iran
  2. 2. Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2016

Abstract

Background: Extended-spectrum beta-lactamase of CTX-M-type is considered as an important mechanism resistant to beta-lactam drugs in the gram-negative pathogens. In this study, the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) as well as the frequency of CTX-M genes from urinary tract infections in inpatients and outpatients caused by Escherichia coli in selected health centers in Isfahan, Iran, was studied in 2013. Methods: The study was carried out on 120 E. coli isolates from inpatients and outpatients with urinary tract infection. The susceptibility of isolates was tested using disc diffusion method. The ESBL-producer strains were confirmed using combined discs method. Finally, the blaCTX-M gene was determined in ESBL-producer isolates using polymerase chain reaction (PCR) method. To identify the strains of E. coli producing CTX-M genes, PCR method was used. The collected data were entered and analyzed in WHONET 5.6 and Excel softwares. Findings: From 120 strains of E. coli, 12 outpatient (20.0%) and 33 inpatient (55.0%) isolates were ESBL-producer. 84.4% and 60.1% of outpatient isolates and 94.9% and 84.8% of inpatient isolates showed resistance to ampicillin and co-trimoxazole, respectively. PCR analysis revealed that 30 isolates had CTX-M gene. Conclusion: The production of ESBL, in particular in isolates of inpatients, is a big threat for use of the broadspectrum cephalosporins. Given the presence of CTX-M gene in the high proportion of the isolates, more molecular and epidemiological studies on pathogenic bacteria in health centers is required to be able to have a common plan to control bacterial resistance. © 2016, Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. Emergence of Bla-Ctx-M-Type Gene in Salmonella Enterica Serotypes Isolated From Patients Stool, Journal of Isfahan Medical School (2011)
2. Considerable Rate of Putative Virulent Phylo-Groups in Fecal Carriage of Extended-Spectrum Β-Lactamase Producing Escherichia Coli, Infection, Genetics and Evolution (2019)
3. Pathogenic Features of Urinary Escherichia Coli Strains Causing Asymptomatic Bacteriuria During Pregnancy, Gene Reports (2022)
4. Investigation of Ndm Metallo-Beta-Lactamase and Cmy-2 Ampc Β-Lactamase Production in Escherichia Coli and Enterobacter Spp. Isolated From Human, Comparative Clinical Pathology (2018)
5. Prevalence and Antibiotic Resistance Pattern of Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli in Clinical Specimens, Journal of Research in Medical Sciences (2019)
6. Prevalence and Antibiotic Susceptibility Patterns of Extended-Spectrum ß-Lactamase and Metallo-ß-Lactamase-Producing Uropathogenic Escherichia Coli Isolates, Laboratory Medicine (2014)
7. Frequency Assessment of Β-Lactamase Enzymes in Escherichia Coli and Klebsiella Isolates in Patients With Urinary Tract Infection, Journal of Research in Medical Sciences (2014)
8. Prevalence of Esbls and Biofilm Formation in Escherichia Coli Isolated From Urinary Tract Infection in Isfahan, Iran, Infection, Epidemiology and Microbiology (2022)
Experts (# of related papers)
View all Related Experts
Jamshid Faghri (14)
Hossein Fazeli (12)
Other Related Docs
9. Frequency and Antibiotic Resistance of Hybrid Entroaggregative/Uropathogenic Escherichia Coli Isolated From Patients Hospitalized in Isfahan, Iran, Infection, Epidemiology and Microbiology (2021)
10. Antibiotic Resistant Pattern in Escherichia Coli Derived From Outpatients and Inpatients With Urinary Tract Infections in Alzahra Hospital, Isfahan, Iran, Journal of Isfahan Medical School (2017)
11. Phenotypic and Molecular Characterization of Extended-Spectrum Β-Lactamase Produced by Escherichia Coli, and Klebsiella Pneumoniae Isolates in an Educational Hospital, Jundishapur Journal of Microbiology (2014)
12. Molecular Epidemiology of Bla Ctx-M Gene-Producing Uropathogenic Escherichia Coli Among Iranian Kidney Transplant Patients: Clonal Dissemination of Cc131 and Cc10, Annals of Clinical Microbiology and Antimicrobials (2021)
13. Prevalence and Antibiotic Resistance Profile of Shiga Toxin-Producing Escherichia Coli Isolated From Diarrheal Samples, Iranian Journal of Microbiology (2020)
14. Prevalence of Blactx-M Gene Among Extended-Spectrum Β-Lactamases Producing Klebsiella Pneumoniae Clinical Isolates in Iran: A Meta-Analysis, Iranian Journal of Medical Sciences (2018)
15. Characterization of Extended-Spectrum Β-Lactamase-Producing Uropathogenic Escherichia Coli Among Iranian Kidney Transplant Patients, Infection and Drug Resistance (2020)
16. Global Evaluation of the Antibacterial Activity of Ceftolozane/Tazobactam Against Esbls-Producing Escherichia Coli and Klebsiella Pneumoniae: A Systematic Review and Meta-Analysis, Therapeutic Advances in Infectious Disease (2023)
17. High Frequency of Integrons and Efflux Pump in Uropathogenic Escherichia Coli Isolated From Iranian Kidney and Non-Kidney Transplant Patients, Gene Reports (2020)
18. Characterization of Hypervirulent Extended-Spectrum Β-Lactamase-Producing Klebsiella Pneumoniae Among Urinary Tract Infections: The First Report From Iran, Infection and Drug Resistance (2020)
19. High Prevalence of Multidrug-Resistance Uropathogenic Escherichia Coli Strains, Isfahan, Iran, Journal of Natural Science, Biology and Medicine (2016)
20. Resistotyping, Phenotyping and Genotyping of New Delhi Metallo-Β-Lactamase (Ndm) Among Gram-Negative Bacilli From Iranian Patients, Journal of Medical Microbiology (2017)
21. Hospital Air: A Potential Route for Transmission of Infections Caused by Β-Lactam–Resistant Bacteria, American Journal of Infection Control (2016)
22. High Frequency of Integron-Associated Cassette Arrays Containing Dfra and Aad Genes in Urinary Isolates of Escherichia Coli in the Southwest of Iran, Molecular Genetics, Microbiology and Virology (2021)
23. Detection of Blactx-M15 and Blaoxa-48 Genes in Gram-Negative Isolates From Neonatal Sepsis in Central of Iran, Iranian Journal of Microbiology (2019)
24. Phylogenetic Group Distribution of Uropathogenic Escherichia Coli and Related Antimicrobial Resistance Pattern: A Meta-Analysis and Systematic Review, Frontiers in Cellular and Infection Microbiology (2022)
25. Isolation and Identification of Carbapenemase Kpc Producing Strains of Enterobacteriaceae and Determination of Their Antibiotic Susceptibility Patterns, Journal of Isfahan Medical School (2013)
26. Investigation of Antimicrobial Susceptibility, Class I and Ii Integrons Among Pseudomonas Aeruginosa Isolates From Hospitalized Patients in Isfahan, Iran, BMC Research Notes (2018)
27. The Emergence of Blaoxa-48 and Blandm Among Esbl-Producing Klebsiella Pneumoniae in Clinical Isolates of a Tertiary Hospital in Iran, Acta Microbiologica et Immunologica Hungarica (2018)
28. Evolutionary Trajectories of Beta-Lactamase Ndm and Dlst Cluster in Pseudomonas Aeruginosa: Finding the Putative Ancestor, Pathogens and Global Health (2024)
29. Molecular Epidemiology of Colistin-Resistant Pseudomonas Aeruginosa Producing Ndm-1 From Hospitalized Patients in Iran, Iranian Journal of Basic Medical Sciences (2019)
30. Molecular Characterization and Phage Typing of Methicillin-Resistant Staphylococcus Aureus Isolated From Clinical Samples in Isfahan, Iran, Jundishapur Journal of Microbiology (2023)
31. The Prevalence and Risk Factors for Urinary Tract Infection in Kidney Transplant Recipients in the First Year After Transplantation, Nephro-Urology Monthly (2022)
32. Investigating of Four Main Carbapenem-Resistance Mechanisms in High-Level Carbapenem Resistant Pseudomonas Aeruginosa Isolated From Burn Patients, Journal of the Chinese Medical Association (2018)
33. First Prevalence of Metallo Beta-Lactamases Producing Enterobacteriacea in Iranian Cancer Patients, Annali di Igiene Medicina Preventiva e di Comunita (2019)
34. Identification of Blaoxa−51−23−58, Blavim, Blandm, and Blaimp Carbapenemase Genes in Acinetobacter Baumannii Isolates From Hospitalized Patients, BMC Research Notes (2024)
35. Molecular Assessment of Microbial Etiology in Urinary Tract Infection in Renal Transplant Patients With Fever, Journal of Isfahan Medical School (2018)
36. Inducible Clindamycin Resistance in Clinical Isolates of Staphylococcus Aureus Due to Erm Genes, Iran, Iranian Journal of Microbiology (2014)
37. Molecular Detection of Extended-Spectrum Β-Lactamases (Esbls) and Biofilm Formation in Uropathogen Klebsiella Pneumoniae in Iran, Medical Journal of the Islamic Republic of Iran (2021)
38. Antibiotic Resistance Pattern of Waterborne Causative Agents of Healthcare-Associated Infections: A Call for Biofilm Control in Hospital Water Systems, Journal of Infection and Public Health (2024)
39. From the Urinary Catheter to the Prevalence of Three Classes of Integrons, Β -Lactamase Genes, and Differences in Antimicrobial Susceptibility of Proteus Mirabilis and Clonal Relatedness With Rep-Pcr, BioMed Research International (2021)
40. High Prevalence of Scc Mec-Associated Phenol-Soluble Modulin Gene in Clinical Isolates of Methicillin-Resistant: Staphylococcus Aureus, Annali di Igiene Medicina Preventiva e di Comunita (2019)
41. Clonal Relatedness Among Imipenem-Resistant Pseudomonas Aeruginosa Isolated From Icu-Hospitalized Patients, Critical Care Research and Practice (2015)
42. Epidemiology of Vim-1-Imipenem Resistant Pseudomonas Aeruginosa in Iran: A Systematic Review and Meta-Analysis, Journal of Research in Medical Sciences (2014)
43. Antimicrobial Resistance in Hospitalized Patients With Community Acquired Urinary Tract Infection in Isfahan, Iran, Archives of Iranian Medicine (2021)
44. Prevalence of Blatem and Blaveb Genes in Multidrug-Resistant Acinetobacter Baumannii Strains Isolated From Burn Wound Infection in Isfahan, Iran, Infection, Epidemiology and Microbiology (2020)
45. Determination of Antibiotic Resistance Pattern and Prevalence of Oxa-Type Carbapenemases Among Acinetobacter Baumannii Clinical Isolates From Inpatients in Isfahan, Central Iran, Infezioni in Medicina (2018)
46. Characterization of Clonal Groups of Antibiotic-Resistant Biofilm-Forming Staphylococcus Aureus Strains Isolated From Patients With Urinary Tract Infections in Isfahan, Iran, Archives of Clinical Infectious Diseases (2024)
47. The Determination of Metallo-Beta-Lactamase Enzymes Prevalence in Pseudomonas Aeruginosa Using Etest and Their Antibiogram Patterns in Kermanshah, Iran, Journal of Kerman University of Medical Sciences (2015)
48. Tracking Antibiotic Resistance Genes and Class 1 Integrons in Escherichia Coli Isolates From Wastewater and Agricultural Fields, Water Science and Technology (2021)
49. Agr Typing of Staphylococcus Aureus Species Isolated From Clinical Samples in Training Hospitals of Isfahan and Shahrekord, BMC Research Notes (2019)
50. Co-Selection of Antibiotic and Disinfectant Resistance in Environmental Bacteria: Health Implications and Mitigation Strategies, Environmental Research (2025)