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Simultaneous Detection of Integrase and Antibiotic Resistance Genes Within Sxt Constin in Vibrio Cholerae O1 El Tor Strains Isolated From Iran Using Multiplex-Pcr



Marashi SMA1 ; Esfahani BN2 ; Tavakoli A2 ; Moghim S2 ; Pourshafie MR3 ; Salehi M4
Authors
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Authors Affiliations
  1. 1. Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol, Iran
  2. 2. Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Departments of Genetics, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Iranian Journal of Basic Medical Sciences Published:2012

Abstract

Objective(s) Amongst the various antibiotic resistant elements in Vibrio. cholerae, SXT constin (SXT-C) is important. We were going to design a quick method for determination of antibiotic resistance gene pattern in SXT-C. Materials and Methods Ninety four V. cholerae O 1 El Tor isolates were used in this study. Antibiotic susceptibility testing, multiplex PCR amplification of SXT-C containing dfrA1, sulII, strB and the int in a multiplex PCR were done. Results Results of our study showed that 92 (97.8%) out of 94 isolates were positive for all above genes. Multiplex PCR results correlated with the antibiotic susceptibility data obtained by using disc diffusion assay. Conclusion Using this multiplex PCR, it would be easily possible to demonstrate the presence of antibiotic resistance in SXT-C which, in turn, allows for a suitable treatment in cholera patients causing reduction in the mortality and morbidity rate of the infected individuals.
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