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The Assessment of Biofilm Formation in Iranian Meat Processing Environments Publisher



Manijeh M1 ; Mohammad J2 ; Roha KK1
Authors
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Authors Affiliations
  1. 1. Department of Biology, Faculty of Science, University of Isfahan, Iran
  2. 2. School of Health, Isfahan University of Medical Sciences, Iran

Source: Research Journal of Microbiology Published:2008


Abstract

The biofilm is consist of microbial cell clusters with a network of internal channels or voids in the Extracellular Polymeric Substances (EPS) and glycoprotein matrix. Biofilms due to special structure and EPS are more resistant to control measures. Biofilms can remain on various surfaces which may assist the survival of pathogenic and spoilage bacteria in the food processing environment, a contributing factor in foodborne disease outbreaks. In this research bacterial strains were isolated by swabbing method from surfaces of meat processing factory environments. More than 60 different species of bacteria isolated from various segment of meat processing plant and the hydrophobicity of isolates measured by Microbial Adhesion Test to Hydrocarbon (MATH) method for screening of isolates. The quantity of biofilm of isolates with high hydrophobicity was determined using microliter plate assay method and ELISA reader machine. Results indicated Bacillus megaterium and Staphylococcus epidermidis with 45 and 33% of hydrophobicity have the highest potential in biofilm formation. Pathogenic S. aureus with 30% of hydrophobicity classified under moderately adherent. B. subtilis with 22% of hydrophobicity considered as weakly adherent. Micrococcus varians and M. roseus with 1 and 5% of hydrophobicity were non adherent. The result from this study highlighted the problems of spread of bacteria. In the development of cleaning and sanitization protocol in meat processing environments, an awareness of these biofilm forming bacteria is essential for the meat processing. © 2008 Academic Journals Inc.
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