Anti-Oxidant Effect of Flavonoids on Hemoglobin Glycosylation Publisher Pubmed



Asgary S¹ ; Naderi GH¹ ; Sarrafzadegan N¹ ; Ghassemi N¹ ; Boshtam M¹ ; Rafie M¹ ; Arefian A¹ Show Affiliations
Source: Pharmaceutica Acta Helvetiae Published:1999

Abstract

A high glucose concentration has been found to lead to the glycosylation of amino groups of lysine residue in proteins. The addition of reducing agent not only prevents this reaction but also reverses it. On the other hand, flavonoids which found in plant sources have antioxidant properties. Since the glycosylation of protein is an oxidation reaction, therefore, antioxidants should be able to prevent this reaction. In this study, the best concentration and time to incubate glucose with hemoglobin was investigated. Then the glycosylation degree of hemoglobin in the presence of flavonoids and their absence was measured by means of a colorimetric method. Different concentration of flavonoids (Quercetin, Rutin, Kaempferol) were used. The preventing effect on hemoglobin glycosylation by the three concentrations; 0.5, 5, 10 μg/ml was estimated as follows: for Rutin; 11%, 27%, 42%, Quercetin; 3%, 37%, 52%, Kaempferol; 10%, 12%, 15% respectively. So, the in vivo effect should be investigated and then plants that containing flavonoids can be utilized to prevent or treat complication of diabetes. Copyright (C) 1999 Elsevier Science B.V.