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Suppressive Role of Viola Odorata Extract on Malignant Characters of Mammosphere-Derived Breast Cancer Stem Cells Publisher Pubmed



Yousefnia S1 ; Naseri D2 ; Seyed Forootan F2, 3 ; Tabatabaeian M4 ; Moattar F5 ; Ghafghazi T5 ; Nasr Esfahani MH2 ; Ghaedi K1, 2
Authors
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Authors Affiliations
  1. 1. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Hezar Jerib Ave., Azadi Sq., Isfahan, 81746-73441, Iran
  2. 2. Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Royan St., Salman St., Khorsagan, Isfahan, 816513-1378, Iran
  3. 3. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran
  4. 4. Department of Surgery and Breast Cancer Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Goldaru Pharmaceutical Company, Isfahan, Iran

Source: Clinical and Translational Oncology Published:2020


Abstract

Background: Mammospheres are breast cancer stem cells (BCSCs) that could be yielded through culturing cells in non-adherent and non-differentiating condition. With regard to therapy resistance of cancer stem cells (CSCs), it is essential to discover efficient approaches targeting CSCs. Viola odorata extract has been considered as a traditional herbal anti-metastatic drug in several cancer cells. Effect of this drug on BCSCs has not been clearly identified. Current study tries to detect and to compare effect of Viola odorata extract on malignant characterization of breast cancer cell lines and BCSCs. Materials and methods: MCF7 and SKBR3 and their derived mammospheres as BCSCs were used and the effect of alcoholic extraction of Viola odorata on apoptosis and malignant characters of MCF7, SKBR3 and their derived BCSCs were analyzed and compared. Results: Viola odorata extract induced cell death in MCF7, SKBR3 and their derived mammospheres through apoptosis without any effects on MCF10A. Also, this extract showed anti-migratory, anti-invasion and anti-colony formation activity in MCF7, SKBR3 and their derived mammospheres which was significantly more in MCF7- and SKBR3-derived mammospheres. Also, this extract decreased size and volume of tumors generated by MCF7, SKBR3 and their derived mammospheres in chicken embryo model. Conclusion: Viola odorata extract exerted anti-cancerous activity on both breast cancer cell lines and their derived BCSCs. Anti-cancerous activity of this extract was significantly more in MCF7-, SKBR3-derived mammospheres in comparison with dedicated cell lines. Data suggest that Viola odorata extract mostly targets cancerous cells, not normal cells with exception in high concentration. It acts in a cell-dependent manner. © 2020, Federacion de Sociedades Espanolas de Oncologia (FESEO).
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