Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Incorporation of Rosuvastatin-Loaded Chitosan/Chondroitin Sulfate Nanoparticles Into a Thermosensitive Hydrogel for Bone Tissue Engineering: Preparation, Characterization, and Cellular Behavior Publisher Pubmed



Rezazadeh M1 ; Parandeh M1 ; Akbari V2 ; Ebrahimi Z1 ; Taheri A1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmaceutics and Novel Drug Delivery System Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Pharmaceutical Development and Technology Published:2019


Abstract

Rosuvastatin (RSV) has been shown to have significant impact on the simulation of bone regeneration after local injection. The current study aimed to develop a localized controlled delivery system from RSV by incorporating RSV-loaded chitosan/chondroitin sulfate (CTS/CS) nanoparticles into thermosensitive Pluronic F127/hyaluronic acid (PF127/HA) hydrogel. RSV-loaded CTS/CS nanoparticles were prepared by ionic gelation, and the impact of various formulation variables was assessed using the Box–Behnken design. Consequently, optimized RSV-loaded nanoparticles were incorporated into the PF127/HA hydrogel. Rheological properties, degradation rates of hydrogels, and the release rate of RSV from hydrogel were examined. Mean particle size, zeta potential, entrapment efficiency, and mean release time of the optimized RSV-loaded nanoparticles were confirmed as 283.2 ± 16 nm, –31.2 ± 6.8 mV, 63.1 ± 4.2%, and 6.14 ± 0.3 h, respectively. The hydrogel containing 3% w/v CTS/CS nanoparticles existed as a solution with low viscosity at room temperature converted to a semisolid upon increasing the temperature to 35 °C. Hydrogel engrafted with CTS/CS showed controlled release of RSV during 48 h with superior in vitro gel stability. As revealed by cytotoxicity and mineralization assays, incorporation of RSV-loaded particles into PF127/HA hydrogel led to improvement in osteoblast viability and proliferation. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
Related Docs
View other Related Docs
1. Comparison the Effects of Chitosan and Hyaluronic Acid-Based Thermally Sensitive Hydrogels Containing Rosuvastatin on Human Osteoblast-Like Mg-63 Cells, Research in Pharmaceutical Sciences (2020)
2. An Injectable Carboxymethyl Chitosan-Methylcellulose-Pluronic Hydrogel for the Encapsulation of Meloxicam Loaded Nanoparticles, International Journal of Biological Macromolecules (2020)
3. Preparation and Characterization of an Injectable Thermosensitive Hydrogel for Simultaneous Delivery of Paclitaxel and Doxorubicin, Research in Pharmaceutical Sciences (2018)
4. Biocompatible Nanocomposite Scaffolds Based on Carrageenan Incorporating Hydroxyapatite and Hesperidin Loaded Nanoparticles for Bone Tissue Regeneration, Polymers for Advanced Technologies (2024)
5. Fabrication and Characterization of Injectable Thermosensitive Hydrogel Containing Dipyridamole Loaded Polycaprolactone Nanoparticles for Bone Tissue Engineering, Journal of Drug Delivery Science and Technology (2021)
6. A Forsterite-Reinforced Polypropylene Fumarate/Methoxy Polyethylene Glycol-Hydrogel Enriched With Flavonoid Nanoparticles Enhances Osteoconductivity, Materials Advances (2024)
7. Preparation, Physicochemical, and Retinal Anti-Angiogenic Evaluation of Poloxamer Hydrogel Containing Dexamethasone/Avastin-Loaded Chitosan-N-Acetyl-L-Cysteine Nanoparticles, International Journal of Biological Macromolecules (2022)
8. Preparation and in Vitro Evaluation of Novel Cross-Linked Chondroitin Sulphate Nanoparticles by Aluminium Ions for Encapsulation of Green Tea Flavonoids, IET Nanobiotechnology (2018)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (46)
Saeed Karbasi (29)
Other Related Docs
9. Injectable Gellan Gum/Lignocellulose Nanofibrils Hydrogels Enriched With Melatonin Loaded Forsterite Nanoparticles for Cartilage Tissue Engineering: Fabrication, Characterization and Cell Culture Studies, Materials Science and Engineering C (2020)
10. Injectable Natural Polymer Compound for Tissue Engineering of Intervertebral Disc: In Vitro Study, Materials Science and Engineering C (2017)
11. Polyhedral Oligomeric Silsesquioxane /Platelets Rich Plasma/Gelrite-Based Hydrogel Scaffold for Bone Tissue Engineering, Current Pharmaceutical Design (2020)
12. Pharmaceutical Evaluation of Atorvastatin-Loaded Nanostructured Lipid Carriers Incorporated Into the Gelatin/Hyaluronic Acid/Polycaprolactone Scaffold for the Skin Tissue Engineering, Journal of Biomaterials Applications (2021)
13. Calcitonin-Loaded Octamaleimic Acid–Silsesquioxane Nanoparticles in Hydrogel Scaffold Support Osteoinductivity in Bone Regeneration, Pharmaceutical Development and Technology (2021)
14. Evaluation of the Effects of Chitosan Nanoparticles on Polyhydroxy Butyrate Electrospun Scaffolds for Cartilage Tissue Engineering Applications, International Journal of Biological Macromolecules (2023)
15. Fabrication and Characterization of 3D-Printed Composite Scaffolds of Coral-Derived Hydroxyapatite Nanoparticles/Polycaprolactone/Gelatin Carrying Doxorubicin for Bone Tissue Engineering, Journal of Materials Science: Materials in Medicine (2024)
16. Biological Evaluation of the Effects of Hyaluronic Acid on Poly (3-Hydroxybutyrate) Based Electrospun Nanocomposite Scaffolds for Cartilage Tissue Engineering Application, Materials Technology (2020)
17. Evaluation Avocado Soybean Unsaponifiables Loaded in Poly (Lactic-Co-Glycolic) Acid/Avocado Soybean Unsaponifiables-Fibrin Nanoparticles Scaffold (New Delivery System) Is an Effective Factor for Tissue Engineering, Advanced Biomedical Research (2021)
18. Sustained-Release of Erythropoietin Using a Novel Injectable Thermosensitive Hydrogel: In Vitro Studies, Biological Activity, and Efficacy in Rats, Pharmaceutical Development and Technology (2021)
19. A New Approach to Fabrication of Cs/Bg/Cnt Nanocomposite Scaffold Towards Bone Tissue Engineering and Evaluation of Its Properties, Applied Surface Science (2015)
20. Evaluation of Mechanical Properties and Cell Viability of Poly (3-Hydroxybutyrate)-Chitosan/Al2o3nanocomposite Scaffold for Cartilage Tissue Engineering, Journal of Medical Signals and Sensors (2019)
21. Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2021)
22. Nanobiomaterials in Periodontal Tissue Engineering, Nanobiomaterials in Hard Tissue Engineering: Applications of Nanobiomaterials (2016)
23. Differential Effects of Rat Adscs Encapsulation in Fibrin Matrix and Combination Delivery of Bdnf and Gold Nanoparticles on Peripheral Nerve Regeneration, BMC Neuroscience (2021)
24. Supramolecular Self-Assembled Nanogels a New Platform for Anticancer Drug Delivery, Current Pharmaceutical Design (2017)
25. A Modular Approach to 3D-Printed Bilayer Composite Scaffolds for Osteochondral Tissue Engineering, Journal of Materials Science: Materials in Medicine (2024)
26. Nanocrystalline Cellulose-Hyaluronic Acid Composite Enriched With Gm-Csf Loaded Chitosan Nanoparticles for Enhanced Wound Healing, Biomedical Materials (Bristol) (2019)
27. Transferrin-Targeted Poly(Butylene Adipate)/Terephthalate Nanoparticles for Targeted Delivery of 5-Fluorouracil in Ht29 Colorectal Cancer Cell Line, Journal of Bioactive and Compatible Polymers (2017)
28. Incorporation of Nanohydroxyapatite and Vitamin D3 Into Electrospun Pcl/Gelatin Scaffolds: The Influence on the Physical and Chemical Properties and Cell Behavior for Bone Tissue Engineering, Polymers for Advanced Technologies (2018)
29. Biodegradable Nanocomposite Coatings Accelerate Bone Healing: In Vivo Evaluation, Dental Research Journal (2015)
30. A Novel Flexible, Conductive, and Three-Dimensional Reduced Graphene Oxide/Polyurethane Scaffold for Cell Attachment and Bone Regeneration, Materials and Design (2022)
31. Regeneration of Rat Sciatic Nerve Using Plga Conduit Containing Rat Adscs With Controlled Release of Bdnf and Gold Nanoparticles, Journal of Molecular Neuroscience (2021)
32. Insight Into the Current Directions in Functionalized Nanocomposite Hydrogels, Frontiers in Materials (2020)
33. Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs, Polymers (2022)
34. In Vitro Characterisation of a Sol–Gel Derived in Situ Silica-Coated Silicate and Carbonate Co-Doped Hydroxyapatite Nanopowder for Bone Grafting, Materials Science and Engineering C (2017)
35. Polycaprolactone/Gelatin/Hydroxyapatite Nanocomposite Scaffold Seeded With Stem Cells From Human Exfoliated Deciduous Teeth to Enhance Bone Repair: In Vitro and in Vivo Studies, Materials Technology (2022)
36. Controlled Gentamicin- Strontium Release As a Dual Action Bone Agent: Combination of the Porous Titanium Scaffold and Biodegradable Polymers, Journal of Alloys and Compounds (2017)
37. Poly(Glycerol Sebacate) Nanoparticles for Ocular Delivery of Sunitinib: Physicochemical, Cytotoxic and Allergic Studies, IET Nanobiotechnology (2019)
38. Preparation and Characterization of 3D Nanocomposite Scaffold From Bioactive Glass/Β-Tricalcium Phosphate Via Robocasting Method for Bone Tissue Engineering, Journal of Non-Crystalline Solids (2022)
39. Evaluation of the Effects of Decellularized Wharton Jelly Nanoparticles on Polyhydroxy Butyrate-Chitosan Electrospun Scaffolds for Cartilage Tissue Engineering Applications, Journal of Polymers and the Environment (2025)
40. Local Delivery of Usnic Acid Loaded Rhamnolipid Vesicles by Gelatin / Tragacanth Gum / Montmorillonite/ Vanillin Cryogel Scaffold for Expression of Osteogenic Biomarkers and Antimicrobial Activity, Journal of Drug Delivery Science and Technology (2022)
41. Preparation and Characterization of Aligned Porous Pcl/Zein Scaffolds As Drug Delivery Systems Via Improved Unidirectional Freeze-Drying Method, Materials Science and Engineering C (2016)
42. Ciprofloxacin Loaded Alginate/Chitosan and Solid Lipid Nanoparticles, Preparation, and Characterization, Journal of Dispersion Science and Technology (2012)
43. Synthesis, Characterization, in Vitro Bioactivity and Biocompatibility Evaluation of Hydroxyapatite/Bredigite (Ca7mgsi4o16) Composite Nanoparticles, JOM (2016)
44. Polycaprolactone-Chitosan/Multi-Walled Carbon Nanotube: A Highly Strengthened Electrospun Nanocomposite Scaffold for Cartilage Tissue Engineering, International Journal of Biological Macromolecules (2022)
45. In Vivo and in Vitro Evaluation of Poly (Lactide-Co-Glycolide)/Bioactive Glass Nanocomposite Coating, Advanced Materials Research (2014)
46. An Injectable Thermosensitive Hydrogel/Nanomicelles Composite for Local Chemo-Immunotherapy in Mouse Model of Melanoma, Journal of Biomaterials Applications (2022)
47. Protein-Polysaccharide Interactions for the Fabrication of Bioactive-Loaded Nanocarriers: Chemical Conjugates and Physical Complexes, Pharmacological Research (2022)
48. Development and Optimization of Solid Lipid Nanoparticles of Amikacin by Central Composite Design, Journal of Liposome Research (2010)
49. Fabrication and Characterization of Polycaprolactone Fumarate/Gelatin-Based Nanocomposite Incorporated With Silicon and Magnesium Co-Doped Fluorapatite Nanoparticles Using Electrospinning Method, Materials Science and Engineering C (2020)
50. 5Fu-Loaded Pcl/Chitosan/Fe3o4 Core-Shell Nanofibers Structure: An Approach to Multi-Mode Anticancer System, Advanced Pharmaceutical Bulletin (2022)