Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Hyaluronic Acid-Conjugated Gliadin Nanoparticles for Targeted Delivery of Usnic Acid in Breast Cancer: An in Vitro / in Vivo Translational Study Publisher



Farzan M1 ; Varshosaz J1 ; Mirian M2 ; Minaiyan M3 ; Pezeshki A4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Novel Drug Delivery Systems Research Center, Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Pathology Department and Isfahan Medical Students Research Committee (ISRC), School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Drug Delivery Science and Technology Published:2023


Abstract

Usnic acid (UA) has been used as an antitumor drug; however, its hepatotoxicity hinders its therapeutic use. This study aimed to design a targeted nanocarrier loaded with UA for drug delivery to breast cancer tissue. UA was loaded in gliadin nanoparticles (GNPs) using a desolvation method and conjugated with hyaluronic acid (HA) to target CD44 receptors. After determining the physicochemical properties of the NPs, their cytotoxicity and drug uptake were studied in the 4T1 cell line. The antitumor activity and histopathological effects were assessed in vivo in BALB/c mice. HA-conjugated UA-loaded GNPs (HA-UA-GNP) showed a significantly higher cytotoxic effect and cellular uptake in the 4T1 cell line compared to non-targeted UA-GNPs and free UA. When CD44 receptors were saturated, the cytotoxicity of HA-UA-GNPs on 4T1 cells decreased significantly. Tumor growth in mice treated with HA-UA-GNPs was significantly less than in UA-GNPs or free UA treatment groups. The higher cytotoxic effect and cellular uptake of HA-UA-GNPs compared to the equal dosage of non-targeted UA-GNPs and the free drug showed a successful CD44 targeting. Histochemical findings showed a higher necrosis level in the group treated with HA-UA-GNPs compared to those treated with UA-GNPs, which could be due to the targeting procedure. In conclusion, the designed nanoparticulate formulation of gliadin, functionalized with HA, served as an effective approach for the delivery of UA to breast cancer tissue in vitro and in vivo. © 2023
Related Docs
View other Related Docs
1. Cytotoxic Effects of Chitosan/Retinoic Acid/Albumin Targeted Nanoparticles Loaded With Doxorubicin on Human Hepatoma Cells, Journal of Isfahan Medical School (2013)
2. Preparation and Evaluation of Targeted Albumin Lipid Nanoparticles With Lactobionic Acid for Targeted Drug Delivery of Sorafenib in Hepatocellular Carcinoma, Journal of Drug Delivery Science and Technology (2022)
3. Targeted Nanostructured Lipid Carriers for Delivery of Paclitaxel to Cancer Cells: Preparation, Characterization, and Cell Toxicity, Current Drug Delivery (2017)
4. Fabrication of Poly Hydroxybutyrate-Polyethylene Glycol-Folic Acid Nanoparticles Loaded by Paclitaxel, Current Drug Delivery (2016)
5. Msc-Derived Exosomes Enhance the Anticancer Activity of Drugs in 3D Spheroid of Breast Cancer Cells, Journal of Drug Delivery Science and Technology (2024)
6. In Vitro and in Vivo Evaluation of Novel Dtx-Loaded Multifunctional Heparin-Based Polymeric Micelles Targeting Folate Receptors and Endosomes, Recent Patents on Anti-Cancer Drug Discovery (2020)
7. Sonodynamic Therapy Using Dacarbazine-Loaded Ausio2 Nanoparticles for Melanoma Treatment: An In-Vitro Study on the B16f10 Murine Melanoma Cell Line, Ultrasound in Medicine and Biology (2022)
8. Synthesis and in Vitro/In Vivo Characterization of Raloxifene Grafted Poly(Styrene Maleic Acid)-Poly(Amide-Ether-Ester-Imide) Micelles for Targeted Delivery of Docetaxel in G Protein-Coupled Estrogen Receptor Breast Cancer, Anti-Cancer Agents in Medicinal Chemistry (2018)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (43)
Farshid Hassanzadeh (27)
Other Related Docs
9. Boron Phenyl Alanine Targeted Ionic Liquid Decorated Chitosan Nanoparticles for Mitoxantrone Delivery to Glioma Cell Line, Pharmaceutical Development and Technology (2021)
10. Hyaluronic Acid Targeted Metal Organic Framework Based on Iron (Iii) for Delivery of Platinum Curcumin Cytotoxic Agent to Triple Negative Breast Cancer Cell Line, Applied Organometallic Chemistry (2022)
11. Folated Synperonic-Cholesteryl Hemisuccinate Polymeric Micelles for the Targeted Delivery of Docetaxel in Melanoma, BioMed Research International (2015)
12. Anti Her-2 Aptamer Functionalized Gold Nanoparticles of Dasatinib for Targeted Chemo-Radiotherapy in Breast Cancer Cells, Biomaterials Advances (2023)
13. Multifunctional Plant Virus Nanoparticles for Targeting Breast Cancer Tumors, Vaccines (2022)
14. Gene Delivery by Pei-Nanocomplex Into Breast and Colorectal Tumor Cell Lines, the Impacts of N/P Ratio, Size and Type of the Cell; [Pei-Nanocomplex Tarafindan Meme Ve Kolorektal Tumor Hucre Hatlarinda Gen Dagitimi, N / P Oraninin, Hucrenin Boyutu Ve Turunun Etkileri], Fabad Journal of Pharmaceutical Sciences (2020)
15. A Concise Review on Cancer Treatment Methods and Delivery Systems, Journal of Drug Delivery Science and Technology (2019)
16. Plga-Peg-Ra-Based Polymeric Micelles for Tumor Targeted Delivery of Irinotecan, Pharmaceutical Development and Technology (2018)
17. Chondroitin/Doxorubicin Nanoparticulate Polyelectrolyte Complex for Targeted Delivery to Hepg2 Cells, IET Nanobiotechnology (2017)
18. Dendrimers As Nanoscale Vectors: Unlocking the Bars of Cancer Therapy, Seminars in Cancer Biology (2022)
19. Biotin-Encoded Pullulan-Retinoic Acid Engineered Nanomicelles: Preparation, Optimization and in Vitro Cytotoxicity Assessment in Mcf-7 Cells, Indian Journal of Pharmaceutical Sciences (2016)
20. Development and Optimization of Transferrin-Conjugated Nanostructured Lipid Carriers for Brain Delivery of Paclitaxel Using Box–Behnken Design, Pharmaceutical Development and Technology (2017)
21. Nanoparticles for Targeted Delivery of Therapeutics and Small Interfering Rnas in Hepatocellular Carcinoma, World Journal of Gastroenterology (2015)
22. Boron Phenyl Alanine Targeted Chitosan–Pnipaam Core–Shell Thermo-Responsive Nanoparticles: Boosting Drug Delivery to Glioblastoma in Bnct, Drug Development and Industrial Pharmacy (2021)
23. Multifunctional and Stimuli-Responsive Liposomes in Hepatocellular Carcinoma Diagnosis and Therapy, Translational Oncology (2024)
24. An Injectable Thermosensitive Hydrogel/Nanomicelles Composite for Local Chemo-Immunotherapy in Mouse Model of Melanoma, Journal of Biomaterials Applications (2022)
25. Investigation of Different Targeting Decorations Effect on the Radiosensitizing Efficacy of Albumin-Stabilized Gold Nanoparticles for Breast Cancer Radiation Therapy, European Journal of Pharmaceutical Sciences (2019)
26. Pharmacokinetics, Organ Toxicity and Antitumor Activity of Docetaxel Loaded in Folate Targeted Cholesterol Based Micelles, Current Drug Delivery (2016)
27. Pegylated Multifunctional Ph-Responsive Targeted Polymeric Micelles for Ovarian Cancer Therapy: Synthesis, Characterization and Pharmacokinetic Study, International Journal of Polymeric Materials and Polymeric Biomaterials (2021)
28. Docetaxel-Loaded Nanostructured Lipid Carriers Functionalized With Trastuzumab (Herceptin) for Her2-Positive Breast Cancer Cells, Journal of Liposome Research (2018)
29. Lipoprotein Like Nanoparticles Used in Drug and Gene Delivery, Current Pharmaceutical Design (2016)
30. Cancer Stem Cells (Cscs): The Blockage of Metastatic and Stemness Properties by Metal Nanoparticles, Nanomedicine Research Journal (2021)
31. Folate-Graphene Chelate Manganese Nanoparticles As a Theranostic System for Colon Cancer Mr Imaging and Drug Delivery: In-Vivo Examinations, Journal of Drug Delivery Science and Technology (2019)
32. Cytotoxic Effects of Targeted Galactosylated Pluronic F127/Pluronicl122 Nano-Sized Micelles Loaded With Doxorubicin in Human Hepatocellular Carcinoma Cell Line, Journal of Isfahan Medical School (2013)
33. Cytotoxic Effect of Pluronic F127-Poly (Methyl Vinyl Ether-Alt-Maleic Acid) Micelles Loaded With Doxorubicin on Mcf-7 Cells, Journal of Isfahan Medical School (2013)
34. Tissue Distribution and Systemic Toxicity Evaluation of Raloxifene Targeted Polymeric Micelles of Poly (Styrene-Maleic Acid)-Poly (Amide- Ether-Ester-Imide)-Poly (Ethylene Glycol) Loaded With Docetaxel in Breast Cancer Bearing Mice, Recent Patents on Anti-Cancer Drug Discovery (2019)
35. Fluorescence Resonance Energy Transfer Monitoring of Ph-Responsive Doxorubicin Release From Carbon Dots/Aptamer Functionalized Magnetic Mesoporous Silica, Nanomedicine (2021)
36. Biodegradable and Biocompatible Subcutaneous Implants Consisted of Ph-Sensitive Mebendazole-Loaded/Folic Acid-Targeted Chitosan Nanoparticles for Murine Triple-Negative Breast Cancer Treatment, Journal of Nanobiotechnology (2022)
37. Co-Delivery of Carboplatin and Doxorubicin Using Zif-8 Coated Chitosan-Poly(N-Isopropyl Acrylamide) Nanoparticles Through a Dual Ph/Thermo Responsive Strategy to Breast Cancer Cells, International Journal of Biological Macromolecules (2024)
38. Luteinizing Hormone-Releasing Hormone Targeted Poly(Methyl Vinyl Ether Maleic Acid) Nanoparticles for Doxorubicin Delivery to Mcf-7 Breast Cancer Cells, IET Nanobiotechnology (2016)
39. A Novel Mixed Polymeric Micelle for Co-Delivery of Paclitaxel and Retinoic Acid and Overcoming Multidrug Resistance: Synthesis, Characterization, Cytotoxicity, and Pharmacokinetic Evaluation, Drug Development and Industrial Pharmacy (2018)
40. Folate-Targeted Polyacrylamide/Punicic Acid Nanomicelles for Flutamide Delivery in Prostate Cancer: Characterization, in Vitro Biological Evaluation, and Its Dft Study, Recent Patents on Nanotechnology (2020)
41. As1411 Aptamer Conjugated Gold Nanoclusters As a Targeted Radiosensitizer for Megavoltage Radiation Therapy of 4T1 Breast Cancer Cells, RSC Advances (2018)
42. Lipid Nanocapsules for Imatinib Delivery: Design, Optimization and Evaluation of Anticancer Activity Against Melanoma Cell Line, Iranian Journal of Pharmaceutical Research (2019)
43. Synthesis and in Vitro Evaluation of Self-Assembling Biocompatible Heparin-Based Targeting Polymeric Micelles for Delivery of Doxorubicin to Leukemic Cells, Research in Pharmaceutical Sciences (2025)
44. Ph-Responsive, Adorned Nanoniosomes for Codelivery of Cisplatin and Epirubicin: Synergistic Treatment of Breast Cancer, ACS Applied Bio Materials (2022)
45. Advances in Aptamer-Based Drug Delivery Vehicles for Cancer Therapy, Biomaterials Advances (2022)
46. Uptake of Etoposide in Ct-26 Cells of Colorectal Cancer Using Folate Targeted Dextran Stearate Polymeric Micelles, BioMed Research International (2014)
47. Co-Delivery of Rituximab Targeted Curcumin and Imatinib Nanostructured Lipid Carriers in Non-Hodgkin Lymphoma Cells, Journal of Liposome Research (2021)
48. Optimisation of Processing Variables Effective on Self-Assembly of Folate Targeted Synpronic-Based Micelles for Docetaxel Delivery in Melanoma Cells, IET Nanobiotechnology (2015)
49. Her-2 Aptamer-Targeted Ecoflex® Nanoparticles Loaded With Docetaxel Promote Breast Cancer Cells Apoptosis and Anti-Metastatic Effect, IET Nanobiotechnology (2019)
50. Assessment of Ph Responsive Delivery of Methotrexate Based on Phema-St-Peg-Da Nanohydrogels, Macromolecular Research (2021)