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Evaluation of Lymphocyte Activation Gene3 (Lag-3) on Regulatory T Cells in Patients With Multiple Sclerosis by Intervention Effect of Fingolimod



Sedaghat N1 ; Zarkeshesfahani SH2 ; Alsahebfosoul F3 ; Etemadifar M4 ; Ostadi V5 ; Kianpour F5 ; Akbari M6
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Authors Affiliations
  1. 1. Department of Immunology, School of Medicine AND Isfahan MS and Neuroimmunology Research Center AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
  3. 3. Department of Immunology, School of Medicine AND Isfahan MS and Neuroimmunology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Neurology, School of Medicine AND Isfahan MS and Neuroimmunology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Epidemiology, School of Health and Nutrition, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Journal of Isfahan Medical School Published:2016

Abstract

Background: Regulatory CD4+Foxp3+ T cells (Tregs) play an important role in the prevention of autoimmune disease. It is also suggested that in multiple sclerosis (MS), a central nervous system disease, an aberrant Treg function may play a role. Lymphocyte activation gene3 (LAG-3) as a surface marker of Treg cells with negative regulation of the immune system can effectively encounter with effector lymphocytes. In this study, we evaluated frequency of CD4+Foxp3+LAG-3+ T cells before and after treatment with Fingolimod. Methods: Blood samples was obtained from 20 patients with multiple sclerosis before and after 1 months of Fingolimod treatment, and from 12 age-matched healthy control subjects. Then pripheral blood mononuclear cell (PBMCs) were isolated and the frequency of Treg cells expressing LAG-3 were determined by flowcytometry. Findings: The frequency of CD4+Foxp3+LAG3+ T cells in the peripheral blood of MS patients was increased when compared to baseline (P = 0.005). Conclusion: Our findings suggested that Fingolimod not only increases CD4+Foxp3+ Treg cells, but also is able to enhance expression of LAG-3 on their surface. This is a new finding for the Fingolimod on the immune system. © 2016, Journal of Isfahan Medical School. All rights reserved.
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