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Evaluating T-Cell Immunoglobulin Mucin-3 (Tim-3) Receptor in the Growth Inhibition of Acute Lymphoblastic Leukemia (All) Cell Lines



Zargarbalajam N1 ; Shabani M2 ; Haghighi M3 ; Bayat AA2 ; Aghaei M4
Authors
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Authors Affiliations
  1. 1. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Monoclonal Antibody Research Center, Avicenna Research Institute, The Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
  3. 3. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences AND Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2015

Abstract

Background: T-cell immunoglobulin-mucin (TIM) is a cell-surface and transmembrane glycoprotein. TIM-3 plays a pivotal role in proliferation, invasion and metastasis of tumor cells. The present study was designed to evaluate the expressions of the TIM-3 and the role of the galectin-9, as TIM-3 ligand, in the regulation of cell proliferation in human acute lymphoblastic leukemia (ALL) cell lines. Methods: The expression level of TIM-3 was examined in the Jurkat and KE-37 cell lines using realtime polymerase chain reaction method. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] viability test was used to study the cell proliferation effect of galectin-9. Findings: TIM-3 mRNAs were detected in the both Jurkat and KE-37 cell lines. The expression of TIM-3 in Jurkat cell line was higher than KE-37 cell line (P < 0.001). The MTS assay revealed that galectin-9 reduced cells proliferation in a dose-dependent manner (> 1 nM) in the both cell lines (P < 0.050). Conclusion: The present investigation introduced a possible mechanism for the control of acute lymphoblastic leukemia cell proliferation through TIM-3 and demonstrated that galectin-9 can inhibit the proliferation of Jurkat and KE-37 cell lines. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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