Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of the Effect of Tim-3 Suppression by Mir-498 and Its Effect on Apoptosis and Proliferation Rate of Hl-60 Cell Line Publisher Pubmed



Moghaddam Y1 ; Andalib A1 ; Mohammadganji M1 ; Homayouni V2 ; Sharifi M3 ; Ganjalikhanihakemi M1, 2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Acqured Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran

Source: Pathology Research and Practice Published:2018


Abstract

Background: Acute Myeloid Leukemia (AML) is a Cancer of hematopoietic stem cells with a rapid progression. TIM-3 is expressed on leukemic stem cells (LSCs) in most types of AML and might have a positive effect on maintenance of malignant phenotype. MicroRNAs play important roles in either cancer progression or suppression. In this study were evaluated, the inhibitory effect of miR-498 on TIM-3 expression and its impact on proliferation and survival of HL-60 cell line. Methods: Firstly, the probable inhibitory effect of miR-498 on TIM-3 expression was predicted. HL-60 cells were cultured and expression of TIM-3 was induced on the cells using phorbol miristate acetate. The cells were transfected with miR-498 and expression level of TIM-3 were measured using with q-RT-PCR and flow cytometry methods. In addition, the effect of suppression of TIM-3 expression in HL-60 cell line was analyzed with apoptosis and cell proliferation assays. Results: Bioinformatics analyses predicted that miR-498 has high ability to silence TIM-3 gene expression. Our experiments confirmed that miR-498 was able to strongly silence TIM-3 expression (68% silencing) in HL-60 cell line (P < 0.002). Also, the cells with suppressed expression of TIM-3 had a lower proliferation and higher apoptosis rates. Conclusion: Based on our results, the miR-498 can effectively suppress TIM-3 expression in the AML cell line. TIM-3 suppression, in turn, inhibits malignant cell proliferation and induces its apoptosis. Collectively, suppression of TIM-3 by miR-498 can be considered as a potential powerful way for treatment of AML. © 2018 Elsevier GmbH
Related Docs
View other Related Docs
1. Evaluation of Silencing Effect of Mir-133A-5P Mimic on Tim-3 Expression in Aml (Hl-60) Cell Line, Indian Journal of Clinical Biochemistry (2020)
2. The Mir-125A-3P Inhibits Tim-3 Expression in Aml Cell Line Hl-60 in Vitro, Indian Journal of Hematology and Blood Transfusion (2017)
3. Tim-3 in Leukemia; Immune Response and Beyond, Frontiers in Oncology (2021)
4. Silencing of Tim-3 Expression by Mir-326 Affects Apoptosis and Proliferation of Human Hl-60 Leukemia Cell Line, UHOD - Uluslararasi Hematoloji-Onkoloji Dergisi (2018)
5. Negative Regulation of Tim-3 Expression in Aml Cell Line (Hl-60) Using Mir-330-5P, British Journal of Biomedical Science (2016)
6. The Role of Tim-3 in Hepatocellular Carcinoma: A Promising Target for Immunotherapy?, Frontiers in Oncology (2020)
7. Blockage of Mir-92A-3P With Locked Nucleic Acid Induces Apoptosis and Prevents Cell Proliferation in Human Acute Megakaryoblastic Leukemia, Cancer Gene Therapy (2016)
8. Inducing Cell Proliferative Prevention in Human Acute Promyelocytic Leukemia by Mir-182 Inhibition Through Modulation of Casp9 Expression, Biomedicine and Pharmacotherapy (2017)
Experts (# of related papers)
View all Related Experts
Mohammadreza Sharifi (31)
Mazdak Ganjalikhani Hakemi (12)
Other Related Docs
9. Inhibition of Microrna Mir-92A Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia; [Mikrorna Mir-92A Inhibisyonu Insan Akut Promyelositik Losemide Hucre Proliferasyonunu Inhibe Eder], Turkish Journal of Hematology (2013)
10. Tim-3/Gal-9 Interaction Affects Glucose and Lipid Metabolism in Acute Myeloid Leukemia Cell Lines, Frontiers in Immunology (2023)
11. The Role of Bone Marrow Microenvironment (Bmm) Cells in Acute Myeloid Leukemia (Aml) Progression: Immune Checkpoints, Metabolic Checkpoints, and Signaling Pathways, Cell Communication and Signaling (2023)
12. Dysregulated Expression and Functions of Microrna-330 in Cancers: A Potential Therapeutic Target, Biomedicine and Pharmacotherapy (2022)
13. Differential Expression Profile of Mir-27B, Mir-29A, and Mir-155 in Chronic Lymphocytic Leukemia and Breast Cancer Patients, Molecular Therapy Oncolytics (2020)
14. Tim-3/Galectin-9 Interaction and Glutamine Metabolism in Aml Cell Lines, Hl-60 and Thp-1, BMC Cancer (2024)
15. Microrna in Leukemia: Tumor Suppressors and Oncogenes With Prognostic Potential, Journal of Cellular Physiology (2019)
16. Inhibition of Microrna Mir-92A Induces Apoptosis and Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia Through Modulation of P63 Expression, Molecular Biology Reports (2014)
17. Inducing Apoptosis and Decreasing Cell Proliferation in Human Acute Promyelocytic Leukemia Through Regulation Expression of Casp3 by Let-7A-5P Blockage, Indian Journal of Hematology and Blood Transfusion (2018)
18. Circulating Microrna-192 As a Diagnostic Biomarker in Human Chronic Lymphocytic Leukemia, Cancer Gene Therapy (2016)
19. Antiproliferative Effect of Upregulation of Hsa-Let-7C-5P in Human Acute Erythroleukemia Cells, Cytotechnology (2018)
20. Effect of Hypoxia on Mir-21 and Mir-130A Expression in Murine Adipose-Derived Mesenchymal Stem Cells in Primary and Immortality Phases, Journal of Isfahan Medical School (2016)
21. Apoptosis Induction in Acute Promyelocytic Leukemia Cells Through Upregulation of Cebpα by Mir-182 Blockage, Molecular Biology Research Communications (2018)
22. Therapeutic Inhibition of Microrna-21 (Mir-21) Using Locked-Nucleic Acid (Lna)-Anti-Mir and Its Effects on the Biological Behaviors of Melanoma Cancer Cells in Preclinical Studies, Cancer Cell International (2020)
23. Degradation of Mir-21 Induces Apoptosis and Inhibits Cell Proliferation in Human Hepatocellular Carcinoma, Cancer Gene Therapy (2015)
24. Downregulation of Mir100hg Induces Apoptosis in Human Megakaryoblastic Leukemia Cells, Indian Journal of Hematology and Blood Transfusion (2021)
25. Microrna Dysregulation and Its Impact on Apoptosis-Related Signaling Pathways in Myelodysplastic Syndrome, Pathology Research and Practice (2024)
26. Circulating Lncrna Hotair Is a Biomarker for Pediatric Acute Lymphoblastic Leukemia and Mediator of Mir-326 Exosomal Export, Scientific Reports (2025)
27. Single Cell Rna Sequencing Improves the Next Generation of Approaches to Aml Treatment: Challenges and Perspectives, Molecular Medicine (2025)
28. Mir-370 Expression Analysis in Breast Cancer, Journal of Isfahan Medical School (2015)
29. The Prominent Role of Mir-942 in Carcinogenesis of Tumors, Advanced Biomedical Research (2022)
30. Circulating Micrornas in Hepatocellular Carcinoma: Potential Diagnostic and Prognostic Biomarkers, Current Pharmaceutical Design (2016)
31. Study of T-Cell Immunoglobulin and Mucin Domain-3 Expression Profile in Peripheral Blood and Bone Marrow of Human Acute Lymphoblastic Leukemia Patients, Journal of Research in Medical Sciences (2020)
32. Inhibition of Microrna Mir-222 With Lna Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia, Indian Journal of Hematology and Blood Transfusion (2017)
33. Apoptosis-Inducing and Antiproliferative Effect by Inhibition of Mir-182-5P Through the Regulation of Casp9 Expression in Human Breast Cancer, Cancer Gene Therapy (2017)
34. Evaluation of the Expressed Mir‑129 and Mir‑549A in Patients With Multiple Sclerosis, Advanced Biomedical Research (2021)
35. Locked Nucleic Acid Anti-Mir-21 Inhibits Cell Growth and Invasive Behaviors of a Colorectal Adenocarcinoma Cell Line: Lna-Anti-Mir As a Novel Approach, Cancer Gene Therapy (2016)
36. Expression of Hsa-Mir-204, Runx2, Pparγ, and Bcl2 in Bone Marrow Derived Mesenchymal Stem Cells From, Cell Journal (2017)
37. Inhibition of Microrna-21 Via Locked Nucleic Acid-Anti-Mir Suppressed Metastatic Features of Colorectal Cancer Cells Through Modulation of Programmed Cell Death 4, Tumor Biology (2017)
38. The Effect of Decitabine on the Expression and Methylation of the Ppp1ca, Btg2, and Pten in Association With Changes in Mir-125B, Mir-17, and Mir-181B in Nalm6 Cell Line, Journal of Cellular Biochemistry (2019)
39. Recent Insights Into the Microrna-Dependent Modulation of Gliomas From Pathogenesis to Diagnosis and Treatment, Cellular and Molecular Biology Letters (2022)
40. Transcription Analysis of a Histones Modifiers Panel Coupled With Critical Tumor Suppressor Genes Displayed Frequent Changes in Patients With Aml.: Mrna Levels of Histones Modifiers and Tsgs in Aml, Current Research in Translational Medicine (2021)
41. Mir-196: Emerging of a New Potential Therapeutic Target and Biomarker in Colorectal Cancer, Molecular Biology Reports (2020)
42. Modulation of Long Non-Coding Rnas and Micrornas by Quercetin As a Potential Therapeutical Approach in Cancer: A Comprehensive Review, Current medicinal chemistry (2025)
43. Semaphorin-3A As an Immune Modulator Is Suppressed by Microrna-145-5P, Cell Journal (2018)
44. Current Status and Perspectives Regarding Lna-Anti-Mir Oligonucleotides and Microrna Mir-21 Inhibitors As a Potential Therapeutic Option in Treatment of Colorectal Cancer, Journal of Cellular Biochemistry (2017)
45. Unveiling Mesenchymal Stem Cells’ Regenerative Potential in Clinical Applications: Insights in Mirna and Lncrna Implications, Cells (2023)
46. Microrna-200C Overexpression in Cancer-Associated Fibroblasts Reduces the Invasive Properties of Breast Tumour Cells, Clinical and Translational Discovery (2022)
47. Comparison of Mir-211 Expression in Murine Melanoma Cell Line and Murine Melanoma Tumor by Real-Time Polymerase Chain Reaction, Journal of Isfahan Medical School (2012)
48. The Significant Role of Micrornas in Gliomas Angiogenesis: A Particular Focus on Molecular Mechanisms and Opportunities for Clinical Application, Cellular and Molecular Neurobiology (2023)
49. Deciphering the Multifaceted Role of Micrornas in Hepatocellular Carcinoma: Integrating Literature Review and Bioinformatics Analysis for Therapeutic Insights, Heliyon (2024)
50. Pathogenic and Therapeutic Roles of Cytokines in Acute Myeloid Leukemia, Cytokine (2021)