Comparative Real-Time Pcr Quantification of Cytomegalovirus in Severe Early Childhood Caries and Caries-Free Children Publisher Pubmed



Nourbakhsh N¹ ; Sadri L² ; Moghim S^{3, 4} ; Mohammadi S⁵
Source: European Archives of Paediatric Dentistry Published:2022

Abstract

Purpose: The present study was undertaken to evaluate cytomegalovirus (CMV) load in carious lesions in children with severe early childhood caries (S-ECC) and compare it with caries-free (CF), healthy children, by quantitative real-time polymerase chain reaction (PCR). Materials and methods: Dental plaque samples were collected from 57 subjects including S-ECC (n = 33) and CF (n = 24) patients. The two groups were matched in terms of age, gender, and socioeconomic status. After deoxyribonucleic acid (DNA) extraction, SYBR Green real-time PCR was used to quantify CMV DNA. Results: The CMV DNA was detected in seven patients in the S-ECC group. The mean of decayed, missing, and filled tooth surfaces (dmfs) was 11.90 ± 5.95 in the S-ECC group. There was no significant difference in gender between the two groups. There was a significant relationship between CMV positivity in children and the S-ECC group (p = 0.043). Conclusion: Results of this study suggest a relationship between the presence of CMV in the oral cavity and S-ECC. We can design clinical trials to confirm these results and improve children's dental care. © 2022, The Author(s), under exclusive licence to European Academy of Paediatric Dentistry.