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Population Structure of Leishmania Tropica Causing Anthroponotic Cutaneous Leishmaniasis in Southern Iran by Pcr-Rflp of Kinetoplastid Dna Publisher Pubmed



Ghatee MA1, 2 ; Mirhendi H3 ; Marashifard M1 ; Kanannejad Z4 ; Taylor WR5, 6 ; Sharifi I7
Authors
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Authors Affiliations
  1. 1. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
  2. 2. Department of Parasitology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
  3. 3. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Mahidol Oxford Tropical Medicine Research Unit, Bangkok, Thailand
  6. 6. Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, United Kingdom
  7. 7. Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran

Source: BioMed Research International Published:2018


Abstract

Iran is one of the six countries with the most cutaneous leishmaniasis (CL) patients. Understanding better the genotypes of the parasite population in relation to geography and climate is critical to achieving better CL control. We aimed to characterise the population structure of Leishmania tropica, the cause of anthroponotic cutaneous leishmaniasis (ACL), from important foci in southeast (Bam and Kerman) and southwest (Shiraz) Iran. A total of 39 L. tropica isolates from ACL patients from southeast (Bam 14, Kerman 12) and southwest (Shiraz 13) Iran were analysed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) of the kinetoplast DNA (kDNA) using restriction enzymes MspI (HpaII) and ClaI. 37 genotypes were identified among south Iran L. tropica isolates. The unweighted pair group method with arithmetic mean (UPGMA) tree obtained from the banding patterns of ClaI digested kDNA RFLP distinguished southeast from and southwest L. tropica isolates with some subclustering but the MspI derived tree showed greater discrimination with greater subclustering and divergence of the two foci of southeast region but with some overlapping. Although a monophyletic structure has been defined for southeast L. tropica, isolates from two foci of southeast Iran were partly discriminated in the current study. Copyright © 2018 Mohammad Amin Ghatee et al.
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