Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Negative Regulation of Semaphorin-3A Expression in Peripheral Blood Mononuclear Cells Using Microrna-497-5P Publisher Pubmed

Summary: Study suggests miR-497-5p suppresses Sema3A, potentially aiding autoimmune disease treatment like MS or arthritis. #AutoimmuneDisease #MicroRNA

Shapoori S1 ; Ganjalikhanihakemi M1 ; Rezaeepoor M1 ; Alsahebfosoul F1 ; Khosravi S2 ; Etemadifar M3 ; Mansourian M4
Authors

Source: Iranian Journal of Medical Sciences Published:2019


Abstract

Background: Semaphorin-3A (Sema3A), as a secreted semaphorin, is an immune modulator molecule participating in the pathogenesis of autoimmune diseases. MicroRNAs (miRNAs) modulate the target-gene expression at the post-transcriptional level. It has been proposed that miRNAs may be crucial to the modulation of the function of semaphorins. Previous findings have proven that miR-497-5p is upregulated and Sema3A is downregulated in some autoimmune disorders. Thus, we aimed to examine the presence of any correlation between Sema3A and miR-497-5p in peripheral blood mononuclear cells (PBMCs). Methods: PBMCs were cultured and transfected with miR-497-5p mimic using the X-tremeGENE™ reagent. The expression level of Sema3A was assessed after 48 hours in supernatants and cells via the enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction, respectively. Cell viability was evaluated using the methylthiazol tetrazolium assay. All the experiments were done in triplicate, and the statistical analyses were performed with SPSS, version 20. P values equal to or less than 0.05 were considered significant. Results: We observed downregulation of the Sema3A gene (P=0.0001) and its secretion (P=0.032) in the PBMCs through miR-497-5p transfection. Moreover, transfection with miR-497-5p mimic and downregulation of Sema3A did not affect the viability of the PBMCs (P=0.061). Conclusion: Based on the obtained results, we suggest that miR-497-5p has a high suppressive effect on Sema3A expression and both Sema3A and miR-497-5p can be considered critical targets for further studies on future therapeutic attempts for the treatment of autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. © 2019, Shiraz University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Semaphorin-3A As an Immune Modulator Is Suppressed by Microrna-145-5P, Cell Journal (2018)
2. Decreased Expression of Sema3a, an Immune Modulator, in Blood Sample of Multiple Sclerosis Patients, Gene (2017)
3. The Role of Micrornas Involved in the Disorder of Blood–Brain Barrier in the Pathogenesis of Multiple Sclerosis, Frontiers in Immunology (2023)
Experts (# of related papers)
View all Related Experts
Mazdak Ganjalikhani Hakemi (6)
Masoud Etemadifar (6)
Negative Regulation of Semaphorin-3A Expression in Peripheral Blood Mononuclear Cells Using Microrna-497-5P
Other Related Docs
4. Comparison of the Expression of Mir-326 Between Interferon Beta Responders and Non-Responders in Relapsing-Remitting Multiple Sclerosis, Cell Journal (2020)
5. Analysis of the Expression of Mir-34A, Mir-199A, Mir-30C and Mir-19A in Peripheral Blood Cd4+T Lymphocytes of Relapsing-Remitting Multiple Sclerosis Patients, Gene (2018)
6. Oleuropein As an Effective Suppressor of Inflammation and Microrna-146A Expression in Patients With Rheumatoid Arthritis, Cell Journal (2023)
7. Risk Factor Effect of Rs1044165 and Rs3745453 As Neighboring Variants of Mir-223, Mir-24, Mir-23A and Mir-27A on the Onset of Ms Disease in Isfahan/Iran, Gene Reports (2018)
8. Differential Expression Profile of Mir-27B, Mir-29A, and Mir-155 in Chronic Lymphocytic Leukemia and Breast Cancer Patients, Molecular Therapy Oncolytics (2020)
9. Blockage of Mir-92A-3P With Locked Nucleic Acid Induces Apoptosis and Prevents Cell Proliferation in Human Acute Megakaryoblastic Leukemia, Cancer Gene Therapy (2016)
10. Negative Regulation of Tim-3 Expression in Aml Cell Line (Hl-60) Using Mir-330-5P, British Journal of Biomedical Science (2016)
11. Microrna-29B Variants and Mxa Expression Change During Interferon Beta Therapy in Patients With Relapsing-Remitting Multiple Sclerosis, Multiple Sclerosis and Related Disorders (2019)
12. Microrna in Leukemia: Tumor Suppressors and Oncogenes With Prognostic Potential, Journal of Cellular Physiology (2019)
13. Lactobacillus Rhamnosus and Lactobacillus Delbrueckii Ameliorate the Expression of Mir-125A and Mir-146A in Systemic Lupus Erythematosus Patients, Applied Biochemistry and Biotechnology (2024)
14. Circulating Microrna-192 As a Diagnostic Biomarker in Human Chronic Lymphocytic Leukemia, Cancer Gene Therapy (2016)
15. Mir-326 and Mir-26A, Two Potential Markers for Diagnosis of Relapse and Remission Phases in Patient With Relapsing-Remitting Multiple Sclerosis, Gene (2014)
16. Microrna-92A Drives Th1 Responses in the Experimental Autoimmune Encephalomyelitis, Inflammation (2019)
17. Antiproliferative Effect of Upregulation of Hsa-Let-7C-5P in Human Acute Erythroleukemia Cells, Cytotechnology (2018)
18. Upregulation of Cd4+ T-Cell Derived Mir-223 in the Relapsing Phase of Multiple Sclerosis Patients, Cell Journal (2016)
19. Mir-939, As an Important Regulator in Various Cancers Pathogenesis, Has Diagnostic, Prognostic, and Therapeutic Values: A Review, Journal of the Egyptian National Cancer Institute (2024)
20. Microrna Binding Site Polymorphism in Inflammatory Genes Associated With Colorectal Cancer: Literature Review and Bioinformatics Analysis, Cancer Gene Therapy (2020)