Changes in Erythrocyte Sodium-Lithium Countertransport and Plasma Parameters Following Selenium Treatment Publisher



Akbarzadeh S¹ ; Ani M² ; Moshtaghie AA² ; Movahed A¹ Show Affiliations
Source: Journal of Biological Sciences Published:2008

Abstract

The effects of selenium as an antioxidant on erythrocyte Sodium-Lithium Countertransport (SLC) activity and plasma parameters have not already been studied in full detail. In the present study, the relationship between selenium and SLC activity, plasma parameters (lipids, lipoproteins, sodium, potassium, urea and creatinine) was investigated. Male rabbits weighed 1350±50 g were divided in to four groups (5 in each). For in vivo studies selenium dioxide (SeO2) was administered intraperitoneally on alternate days for 2 weeks as acute dose (250 μg kg-1 body weight) and for 7 weeks as chronic dose (125 μg kg-1 body weight). The control groups for each doses received deionized water at the same time. The results showed that the acute dose of selenium decreased SLC activity and Vmax/Km but the Km of the transporter was increased. Also this metal decreased plasma concentrations of cholesterol, triglyceride and VLDL. All these changes may be helpful to the health. The chronic dose of the metal increased SLC activity, Vmax/Km and Vmax, but it decreased Km of the transporter. This dose of selenium increased plasma concentrations of cholesterol, triglyceride, VLDL, LDL, sodium and decreased the potassium level. In vitro studies showed that, the metal at low concentration (1 μM) can lower SLC activity and then it is beneficial to the health, but at high concentration (50 and 100 μM) increases the SLC activity and may cause a serious problem to the health. It is concluded that selenium at high concentration and with chronic dose increases the SLC activity and plasma lipids. But at low concentration and acute dose will have a beneficial effect to the health. © 2008 Asian Network for Scientific Information.