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The Comparison of the Ifn-ɤ, Tnf-Α and Il-10 Cytokines in Healing and Non-Healing Cutaneous Leishmaniasis Publisher



Taraghian M1 ; Hanif H2 ; Mousavi P3 ; Cheshmeh ZB4 ; Samei A5 ; Abdollahi A6 ; Vazini H7
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran
  2. 2. Department of Biology, Faculty of Education, Herat University, Herat, Afghanistan
  3. 3. Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Medical Laboratory Department, Laboratory Expert, University of Medical Sciences, Hamedan, Iran
  5. 5. Department of Laboratory Sciences, School of Allied Medical Sciences, Kashan University of Medical Sciences, Kashan, Iran
  6. 6. Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran
  7. 7. Nursing Department, Basic Sciences Faculty, Hamedan Branch, Islamic Azad University, Hamedan, Iran

Source: Iranian Journal of Parasitology Published:2021


Abstract

Background: Leishmaniasis is one of the main vectors borne and neglected trop-ical parasitic diseases. T cell cytokine responses are highly important in the presen-tations of disease such as control or progression, and understanding of the host immunological response is valuable in diagnosis, follow-up, and vaccine designs. In the current study, the profile of IFN-ɤ, TNF-α, and IL-10 cytokines was inves-tigated through the ELISA technique in PBMCs isolated from antimony re-sistance and susceptible patients. Methods: In this experimental study, 54 patients with healing (n=27) or nonhealing (n=27) CL were recruited. Lesion samples were collected to determine the genotype of Leishmania spp. and peripheral blood mononuclear cells (PBMCs) were obtained to evaluate the cytokines profiles using soluble Leishmania antigen (SLA) and phytohaemagglutinin (PHA) mitogen. Cytokines were assessed by the ELISA technique Results: The IFN-ɤ and TNF-α cytokines were significantly increased in the healing group treated with both SLA antigen and PHA mitogen (P<0.001). The level of IL-10 was significantly increased in non-healing and significantly declined in healing groups (P<0.001). Conclusion: The profile of IFN-ɤ, TNF-α, and IL-10 cytokines are crucially associated with the response of treatment. © 2021 Taraghian et al. Published by Tehran University of Medical Sciences.
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