Changes of Serum Inflammatory Markers After Gamma-Secretase Inhibitor Administration Mice



Khazaei M¹ ; Saeidi H² ; Shabanikia N² ; Kalantari E² ; Tahergorabi Z³
Source: Journal of Isfahan Medical School Published:2013

Abstract

Background: Notch signaling is involved in physiological and pathological processes and gammasecretase inhibitors can suppress it. The aim of this study was to evaluate the effect of DAPT, a gamma secretase inhibitor, on serum inflammatory markers in mice. Methods: 24 C57BL6 mice (age: 5 weeks old) were randomly divided into control and obese groups. Obese group received high-fat diet (60% fat, 20% protein, and 20% carbohydrate) for 20 weeks. Control group received standard diet during this time. Then, each group was divided into receiving and non-receiving DAPT (50mg/kg; Subcutaneous). After 14 days, blood samples were taken for measurements of serum high-sensitive C-reactive protein (hs-CRP) and interlukin-6 (IL-6). Findings: There was no significant difference in serum hs-CRP concentration between control and obese groups (P > 0.05). Administration of DAPT did not alter serum hs-CRP level in both groups (P > 0.05). Serum Il-6 was significantly higher in obese animals compare to controls (P < 0.05) and administration of DAPT reduced serum IL-6 in control and obese animals (P < 0.05). Conclusion: DAPT, as a gamma secretase inhibitor, could affect several physiological and pathological vascular changes by alteration of serum inflammatory markers.