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Changes of Serum Inflammatory Markers After Gamma-Secretase Inhibitor Administration Mice



Khazaei M1 ; Saeidi H2 ; Shabanikia N2 ; Kalantari E2 ; Tahergorabi Z3
Authors
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Authors Affiliations
  1. 1. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Students Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Physiology, School of Medicine and Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2013

Abstract

Background: Notch signaling is involved in physiological and pathological processes and gammasecretase inhibitors can suppress it. The aim of this study was to evaluate the effect of DAPT, a gamma secretase inhibitor, on serum inflammatory markers in mice. Methods: 24 C57BL6 mice (age: 5 weeks old) were randomly divided into control and obese groups. Obese group received high-fat diet (60% fat, 20% protein, and 20% carbohydrate) for 20 weeks. Control group received standard diet during this time. Then, each group was divided into receiving and non-receiving DAPT (50mg/kg; Subcutaneous). After 14 days, blood samples were taken for measurements of serum high-sensitive C-reactive protein (hs-CRP) and interlukin-6 (IL-6). Findings: There was no significant difference in serum hs-CRP concentration between control and obese groups (P > 0.05). Administration of DAPT did not alter serum hs-CRP level in both groups (P > 0.05). Serum Il-6 was significantly higher in obese animals compare to controls (P < 0.05) and administration of DAPT reduced serum IL-6 in control and obese animals (P < 0.05). Conclusion: DAPT, as a gamma secretase inhibitor, could affect several physiological and pathological vascular changes by alteration of serum inflammatory markers.
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