Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Protective Effects of Omega3 on Ubiquitination and Protamination of Rat Sperm After Bleomycin, Etoposide, and Cisplatin Treatment Publisher Pubmed



Hashemi F1 ; Razavi S1 ; Khadivi F1, 2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Nutrition and Cancer Published:2018


Abstract

The purpose of this study is evaluation the protective effect of omega3 on rat sperm protamination and ubiquitination after treatment with Bleomycin, Etoposide and Cisplatin (BEP) drugs. In present study, 40 male rats were divided into four groups: Control, BEP, Omega3 and BEP + Omega3. Sperm protamination and ubiquitination were assessed using chromomycin A3 and immunofluorescence staining respectively. The mean percentage of ubiquitinated sperm in BEP group was significantly increased relative to control group (P <.001). But, the mean percentage of sperm protamination significantly decreased in BEP group relative to control group (P <.001). Rats in BEP + Omega3 group showed a significantly decreased in the mean percentage of sperm ubiquitination as compared to BEP group (P <.05) while, sperm protamination increased significantly relative to BEP group (P <.001). Administration of Omega3 after chemotherapy showed an improvement in sperm ubiquitination and protamination. Our data indicated that omega3 after chemotherapy may be beneficial for chromatin remodeling during spermatogenesis following BEP treatment. © 2018, © 2018 Taylor & Francis Group, LLC.
Related Docs
View other Related Docs
1. Improvement of Rat Sperm Chromatin Integrity and Spermatogenesis With Omega 3 Following Bleomycin, Etoposide and Cisplatin Treatment, Nutrition and Cancer (2021)
2. Protective Effects of Zinc on Rat Sperm Chromatin Integrity Involvement: Dna Methylation, Dna Fragmentation, Ubiquitination and Protamination After Bleomycin Etoposide and Cis-Platin Treatment, Theriogenology (2020)
3. Effect of Omega-3 on Rat Sperm Dna Methylation and Histological Structure of Testis After Treatment With Bleomycin, Etoposide and Cisplatin (Bep), Journal of Babol University of Medical Sciences (2019)
4. Effect of Zinc on Spermatogenesis and Sperm Chromatin Condensation in Bleomycin, Etoposide, Cisplatin Treated Rats, Cell Journal (2019)
5. Investigating the Correlation Between Ubiquitination With Motility, Morphology, and Dna Methylation in Rat Sperm, Journal of Isfahan Medical School (2017)
6. Effect of Protamine-2 Deficiency on Icsi Outcome, Reproductive BioMedicine Online (2004)
7. Relationship Between Protamine Deficiency With Fertilization Rate and Incidence of Sperm Premature Chromosomal Condensation Post-Icsi, Andrologia (2004)
8. Optimizing Acridine Orange Staining for Assessment of Protective Effects of Saffron and Vitamin E on Rat Sperm Dna Structure, Journal of Isfahan Medical School (2014)
Experts (# of related papers)
View all Related Experts
Shahnaz Razavi (26)
Mohammad Mardani (12)
Other Related Docs
9. Can Sperm Protamine Deficiency Induce Sperm Premature Chromosomal Condensation?, Andrologia (2006)
10. Selection of Sperm Based on Combined Density Gradient and Zeta Method May Improve Icsi Outcome, Human Reproduction (2009)
11. Effect of Varicocelectomy on Semen Parameters and Protamine Deficiency 3 and 6 Months Post Surgery, Journal of Isfahan Medical School (2007)
12. The Role of Sperm Chromatin Anomalies on the Outcome of Assisted Reproductive Techniques, Yakhteh (2006)
13. Effect of Saffron on Rat Sperm Chromatin Integrity, Iranian Journal of Reproductive Medicine (2014)
14. Effect of Sperm Dna Damage and Sperm Protamine Deficiency on Fertilization and Embryo Development Post-Icsi, Reproductive BioMedicine Online (2005)
15. Correlation Between Sperm Motility and Sperm Chromatin/Dna Damage Before and After Cryopreservation and the Effect of Folic Acid and Nicotinic Acid on Post-Thaw Sperm Quality in Normozoospermic Men, Cell and Tissue Banking (2019)
16. Coadministration of Calcium Chloride With Lead Acetate Can Improve Motility of Cauda Epididymal Spermatozoa in Swiss White Mice, International Journal of Reproductive BioMedicine (2016)
17. Evaluation of Sperm Selection Procedure Based on Hyaluronic Acid Binding Ability on Icsi Outcome, Journal of Assisted Reproduction and Genetics (2008)
18. Effect of Polyvinyl Pyrrolidone on Sperm Membrane Integrity and Chromatin Status, International Journal of Fertility and Sterility (2008)
19. Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men During Cryopreservation., Scientific Journal of Kurdistan University of Medical Sciences (2022)
20. Impact of Vitamin C on Gene Expression Profile of Inflammatory and Anti-Inflammatory Cytokines in the Male Partners of Couples With Recurrent Pregnancy Loss, International Journal of Inflammation (2022)
21. Effects of Biotin and Folic Acid on Motility, Viability, Morphology, Chromatin Density and Integrity of Cryopreserved and Thawed Sperm in Normozoospermic Men, Scientific Journal of Kurdistan University of Medical Sciences (2021)
22. Relationship Between Dietary Antioxidant Intake and Sperm Analysis Parameters, Iranian Journal of Obstetrics, Gynecology and Infertility (2018)
23. Effect of Human Sperm Chromatin Anomalies on Fertilization Outcome Post-Icsi, Andrologia (2003)
24. Comparing the Roles of Sperm Chromatin Integrity and Apoptosis in Intrauterine Insemination Outcomes of Couples With Mild Male and Female Factor Infertility, Journal of Reproduction and Infertility (2014)
25. Effect of Cholesterol, Iron and Vitamin E on Protamine Deficiency and Dna Fragmentation of Male Rabbit Sperm, Journal of Isfahan Medical School (2014)
26. A Histopathological Study of Nephrotoxicity, Hepatoxicity or Testicular Toxicity: Which One Is the First Observation As Side Effect of Cisplatin-Induced Toxicity in Animal Model?, Journal of Nephropathology (2012)
27. Cancer Induced Infertility and the Role of L-Carnitine: A Review for Possible Future Clinical Applications, International Journal of Cancer Management (2017)
28. The Correlation Between Sperm Dna Fragmentation and the Expression of Long Non-Coding Rna Hotair in Infertile Men, Journal of Isfahan Medical School (2021)
29. The Effects of Vitamin C and Menthone on Acyclovir Induced Dna Damage in Rat Spermatozoa: An Experimental Study, International Journal of Reproductive BioMedicine (2018)
30. Influence of Sperm Chromatin Anomalies on Assisted Reproductive Technology Outcome, Fertility and Sterility (2009)
31. Credibility of Chromomycin A3 Staining in Prediction of Fertility, International Journal of Fertility and Sterility (2009)
32. Failed Fertilization After Icsi and Spermiogenic Defects, Fertility and Sterility (2008)
33. Effect of Ascorbic Acid and Menthone on the Caspase 3 in the Sperm Cells of Acyclovir Treated Rats, Acta Medica Mediterranea (2016)
34. Varicocelectomy: Semen Parameters and Protamine Deficiency, International Journal of Andrology (2009)
35. Efficiency of Sil-Select and Percoll to Recover Spermatozoa With Normal Chromatin and Morphology and the Effect of These Parameters on Fertilization, Embryo Quality and Cleavage Score, Middle East Fertility Society Journal (2003)
36. Scsa Results Correlated With Rate of Motility Reduction After Ejaculation in Asthenozoospermia, Andrologia (2019)
37. Evaluation of Zeta and Ha-Binding Methods for Selection of Spermatozoa With Normal Morphology, Protamine Content and Dna Integrity, Andrologia (2010)
38. The Comparison of Efficiency of Density Gradient Centrifugation and Zeta Methods in Separation of Mature Sperm With Normal Chromatin Structure, Yakhteh (2009)
39. Effect of Varicocelectomy on Semen Parameters and Sperm Chromatin Status, Yakhteh (2005)
40. Impairment of Sperm Efficiency in Mice Following Short-Term Nano-Titanium Dioxide Exposure: An Experimental Study, International Journal of Reproductive BioMedicine (2021)
41. Altered Expression of Klc3 May Affect Semen Parameters, International Journal of Reproductive BioMedicine (2016)
42. The Effects of Lycopene Supplement on the Spermatogram and Seminal Oxidative Stress in Infertile Men: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial, Phytotherapy Research (2019)
43. Chromomycin A3 Staining As a Useful Tool for Evaluation of Male Fertility, Journal of Assisted Reproduction and Genetics (2000)
44. Effect of Chromatin Condensation on Frozen-Thawed Sperm Dna Integrity in Normozoospermic Men, Scientific Journal of Kurdistan University of Medical Sciences (2019)
45. Can Altered Expression of Hspa2 in Varicocele Patients Lead to Abnormal Spermatogenesis?, International Journal of Fertility and Sterility (2010)