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In Silico Analysis of Transmembrane Protein 31 (Tmem31)Antigen to Design Novel Multiepitope Peptide and Dna Cancer Vaccines Against Melanoma Publisher Pubmed



Safavi A1 ; Kefayat A2 ; Abiri A3 ; Mahdevar E4 ; Behnia AH5 ; Ghahremani F6
Authors
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Authors Affiliations
  1. 1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Oncology, Cancer Prevention Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Medicinal Chemistry, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
  4. 4. Department of Biology, Faculty of Science and Engineering, Science and Arts University, Yazd, Iran
  5. 5. Department of Biology, Faculty of the Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
  6. 6. Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences, Arak, Iran

Source: Molecular Immunology Published:2019


Abstract

Multiepitope cancer vaccines are announcing themselves as the future of melanoma treatment. Herein, high immunogenic regions of transmembrane protein 31 (TMEM31)antigen were selected according to cytotoxic T lymphocytes’ (CTL)epitopes and major histocompatibility complex (MHC)binding affinity through in silico analyses. The 32–62, 77–105, and 125–165 residues of the TMEM31 were selected as the immunodominant fragments. They were linked together by RVRR and HEYGAEALERAG motifs to improve epitopes separation and presentation. In addition, to activate helper T lymphocytes (HTL), Pan HLA DR-binding epitope (PADRE)peptide sequence and tetanus toxin fragment C (TTFrC)were incorporated into the final construct. Also, the Beta-defensin conserved domain was utilized in the final construct as a novel adjuvant for Toll-like receptor 4/myeloid differentiation factor (TLR4-MD)activation. The CTL epitopes, cleavage sites, post-translational modifications, TAP transport efficiency, and B cells epitopes were predicted for the peptide vaccine. The final construct contained multiple CTL and B cell epitopes. In addition, it showed 93.55% and 99.13% population coverage in the world for HLA I and HLA II, respectively. According to these preliminary results, the multiepitope cancer vaccine can be an appropriate choice for further experimental investigations. © 2019 Elsevier Ltd
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