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In-Silico Design of a New Multi-Epitope Vaccine Candidate Against Sars-Cov-2 Publisher



Alibakhshi A1 ; Alagheband Bahrami A2 ; Mohammadi E3, 4 ; Ahangarzadeh S5 ; Mobasheri M6
Authors
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Authors Affiliations
  1. 1. Molecular Medicine Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
  3. 3. Core Research Facilities, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Biotechnology, Faculty of Advanced Sciences and Technology, Tehran Islamic Azad University of Medical Sciences, Tehran, Iran

Source: Acta Virologica Published:2023


Abstract

Frequent, and sometimes more dangerous, mutations in SARS-CoV-2 indicate that a stronger strategy is needed to produce an effective vaccine—a vaccine that contains a wider range of virus factors and remains effective if one or more mutations have occurred in a part of the genome. In this study, four important virus proteins were used to make a multi-epitope protein vaccine. For this purpose, antigenic determinant of 4 proteins were selected and a protein structure was designed using 4 domains containing epitopes. After examining its antigenic potential, its three-dimensional structure was designed and then docked with immune system receptors. Finally, using the dynamic molecular (MD) simulation, complexes and interactions were investigated and their interaction energies were measured. The results of the study showed that the designed structure has good relative stability and interacts well with its receptors and can be used as a vaccine candidate for further studies. Copyright © 2024 Alibakhshi, Alagheband Bahrami, Mohammadi, Ahangarzadeh and Mobasheri.
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