Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Identification of Leishmania Species and Treatment Courses in Patients With Leishmaniasis in Isfahan, Iran



Hejazi SH1 ; Hashemi N2 ; Hashemi M3 ; Abdian N4 ; Shafiei L5 ; Hashemi S6 ; Nilforoushzadeh MA7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Parasitology and Mycology, School of Medicine, Northern Khorasan University of Medical Sciences, Bojnurd, Iran
  3. 3. Department of Statistics, Deputy of Research, Northern Khorasan University of Medical Sciences, Bojnurd, Iran
  4. 4. Department of Parasitology and Mycology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
  5. 5. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Northern Khorasan University of Medical Sciences, Bojnurd, Iran
  7. 7. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Isfahan Medical School Published:2012

Abstract

Background: Leishmaniasis is a parasitic infectious disease with a wide spectrum in tropical and subtropical areas. Antimoniates are compounds of choice to treat cutaneous leishmaniasis in Iran and many other countries of the world. There are many fluctuations in response to leishmanicidal agents according to type of the disease, involved species of the parasite, and drug regimen. Methods: This study was conducted in the Skin Disease and Leishmaniasis Research Center, Isfahan, Iran. Novy-MacNeal-Nicolle (NNN) and Roswell Park Memorial Institute (RPMI) 1640 media were used to isolate parasites from skin lesions of patients. Deoxyribonucleic acid (DNA) extraction of Leishmania isolates was performed and strain identification was determined using specific internal transcribed spacers-1 (ITS-1) primers. After amplification, the resulted products were analyzed using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Patients were under traditional glucantime therapy and were followed up for 14 weeks to detect their treatment courses. Findings: PCR-RELP showed that all etiologic agents of cutaneous leishmaniasis (CL) among referral patients in Isfahan were L. major. The mean duration of ulcer recovery of cases with CL was 58 days. Conclusion: The mean recovery period of patients with zoonotic CL was noticeably shorter than cases with anthroponotic CL.
Related Docs
View other Related Docs
1. Pcr-Rflp Diagnosis and Characterization of Leishmania Species Causing Human Cutaneous Leishmaniasis and Evaluation of Treatment Times With Glucantime in These Patients, Tropical Biomedicine (2016)
2. Detection of Leishmania Parasites From Cutaneous Leishmaniasis Patients With Negative Direct Microscopy Using Nnn and Pcr-Rflp, Journal of Isfahan Medical School (2012)
3. Comparison of Three Different Therapies for Cutaneous Leishmaniasis and Identification of the Etiologic Isolates in Isfahan, Iran, Archives of Iranian Medicine (2020)
4. Comparative Molecular Epidemiology of Leishmania Major and Leishmania Tropica by Pcr-Rflp Technique in Hyper Endemic Cities of Isfahan and Bam, Iran, Case Reports and Clinical Practice Review (2010)
5. Comparative Molecular Epidemiology of Leishmania Major and Leishmania Tropica by Pcr-Rflp Technique in Hyper Endemic Cities of Isfahan and Bam, Iran, Medical Science Monitor (2010)
6. Identification of Leishmania Isolates From Healing and Nonhealing Cutaneous Leishmaniasis Patients Using Internal Transcribed Spacer Region Pcr, Jundishapur Journal of Microbiology (2014)
7. Identification and Isolation of the Cause of Cutaneous Leishmaniasis in Isfahan Using Its-Pcr Method, Journal of Isfahan Medical School (2012)
8. Identification and Isolation of the Cause of Cutaneous Leishmaniasis in Isfahan Using Its-Pcr Method, Journal of Isfahan Medical School (2012)
Experts (# of related papers)
View all Related Experts
Seyed Hossein Hejazi (66)
Sedighe Saberi (18)
Other Related Docs
9. The Molecular Epidemiology of Crithidia Strains in Isfahan City and Surrounding Areas, Iran, Journal of Isfahan Medical School (2014)
10. Genetic Variation in Rpoiils Gene Encoding Rna Polymerase Ii Largest Subunit From Leishmania Major, Molecular Biology Reports (2014)
11. Genetic Polymorphism of Leishmania Major in Two Hyper Endemic Regions of Iran Revealed by Ppip-Pcr and Its- Rflp, Archives of Iranian Medicine (2012)
12. Molecular Epidemiological Survey of Cutaneous Leishmaniasis in Two Highly Endemic Metropolises of Iran, Application of Fta Cards for Dna Extraction From Giemsa-Stained Slides, Jundishapur Journal of Microbiology (2016)
13. Effect of Combination Therapy With Systemic Glucantime and Pentoxifylline in the Treatment of Cutaneous Leishmaniasis, International Journal of Dermatology (2006)
14. Molecular Diagnosis of Old World Leishmaniasis: Real-Time Pcr Based on Tryparedoxin Peroxidase Gene for the Detection and Identification of Leishmania Spp, Journal of Vector Borne Diseases (2012)
15. Evaluation of the 11-Year Trend of Cutaneous Leishmaniasis in Six Main Foci of the Disease in Isfahan Province During the Years 2008 to 2018, Journal of Isfahan Medical School (2022)
16. The Epidemiology of Cutaneous Leishmaniasis in Isfahan Province, Iran, During 2001-2011, Journal of Isfahan Medical School (2015)
17. Treatment of Recurrent Cutaneous Leishmaniasis, International Journal of Dermatology (1995)
18. The Efficacy of Intralesional Meglumine Antimoniate (Glucantime) Versus a Combination of Topical Trichloroacetic Acid 50% and Local Heat Therapy by Non-Ablative Radiofrequency on Cutaneous Leishmaniasis Lesions, Journal of Isfahan Medical School (2012)
19. Antimonial Treatment Failure Rate in Patients With Cutaneous Leishmaniasis, Tehran University Medical Journal (2018)
20. A Randomized, Double-Blind, Placebo-Controlled Clinical Trial of Itraconazole in the Treatment of Cutaneous Leishmaniasis, Journal of the European Academy of Dermatology and Venereology (2005)
21. Intraspecific Variation in Leishmania Major Isolated From Different Forms of Zoonotic Cutaneous Leishmaniasis, Iranian Journal of Medical Sciences (2005)
22. Molecular Identification of Leishmania Species in Phlebotomus Alexandri (Diptera: Psychodidae) in Western Iran, Journal of Arthropod-Borne Diseases (2020)
23. Genetic Analysis of Clinical Isolates of Leishmania Major From Isfahan, Iran, Journal of Vector Borne Diseases (2012)
24. Molecular Identification of Leishmania Rna Virus in Cutaneous Leishmaniasis Patients and Rodent Reservoirs in Isfahan Province, Iran, Infection, Genetics and Evolution (2022)
25. In Vitro Antileishmanial Activity of Falcaria Vulgaris Fractions on Leishmania Major, Jundishapur Journal of Natural Pharmaceutical Products (2017)
26. Comparison Between the Efficacy of Photodynamic Therapy and Topical Paromomycin in the Treatment of Old World Cutaneous Leishmaniasis: A Placebo-Controlled, Randomized Clinical Trial, Journal of Isfahan Medical School (2012)
27. A New Focus of Zoonotic Cutaneous Leishmaniasis in Shiraz, Iran, Transactions of the Royal Society of Tropical Medicine and Hygiene (2009)
28. Emergence of Cutaneous Leishmaniasis Due to Leishmania Major in a New Focus of Central Iran, Transactions of the Royal Society of Tropical Medicine and Hygiene (2009)
29. Preparation and Evaluation of Clinical Effects of Glucantime Gel Mask, Journal of Isfahan Medical School (2013)
30. Regulatory T-Cell Profile in Early and Late Lesions of Cutaneous Leishmaniasis Due to Leishmania Major, Journal of Research in Medical Sciences (2012)
31. Clinical Picture of Cutaneous Leishmaniasis in Isfahan, Iran, International Journal of Dermatology (1994)
32. Isolation of Bacteria Causing Secondary Bacterial Infection in the Lesions of Cutaneous Leishmaniasis, Indian Journal of Dermatology (2008)
33. Evaluation of Leishmanin Skin Test and Its Relationship With the Clinical Form and Duration of Cutaneous Leishmaniasis, Dermatology Online Journal (2006)
34. Comparative Effect of Topical Trichloroacetic Acid and Intralesional Meglumine Antimoniate in the Treatment of Acute Cutaneous Leishmaniasis, International Journal of Pharmacology (2006)
35. Evaluation of Leishmanin Skin Test Reaction in Different Variants of Cutaneous Leishmaniasis, Indian Journal of Dermatology (2013)
36. Leishmania Spp. Infection in Rhombomys Opimus and Meriones Libycus As Main Reservoirs of Zoonotic Cutaneous Leishmaniasis in Central Parts of Iran: Progress and Implications in Health Policy, Acta Tropica (2022)
37. The Skin Side Effects of Topical Glucantime in Patients With Cutaneous Leishmaniasis, Journal of Isfahan Medical School (2015)
38. A Comparison Between the Effects of Glucantime, Topical Trichloroacetic Acid 50% Plus Glucantime, and Fractional Laser Plus Glucantime on Cutaneous Leishmaniasis Lesions, Journal of Isfahan Medical School (2013)
39. Molecular Identification and Phylogenetic Classification of Leishmania Spp. Isolated From Human Cutaneous Leishmaniasis in Iran: A Cross-Sectional Study, Iranian Journal of Parasitology (2018)
40. The Efficacy of 5% Trichloroacetic Acid Cream in the Treatment of Cutaneous Leishmaniasis Lesions, Journal of Dermatological Treatment (2012)
41. Bacterial Superinfection in Zoonotic Cutaneous Leishmaniasis, Medical Science Monitor (2012)
42. An Outbreak of Cutaneous Leishmaniasis Due to Leishmania Major in an Endemic Focus in Central Iran, Journal of Parasitic Diseases (2022)
43. Epidemiological Aspects of Cutaneous Leishmaniasis in Iran, Archives of Clinical Infectious Diseases (2015)
44. Aquaglyceroporin1 Gene Expression in Antimony Resistance and Susceptible Leishmania Major Isolates, Journal of Vector Borne Diseases (2016)
45. Population Structures of Leishmania Infantum and Leishmania Tropica the Causative Agents of Kala-Azar in Southwest Iran, Parasitology Research (2018)
46. Regulatory T-Cell Profile in Early and Late Lesions of Cutaneous Leishmaniasis Due to Leishmania Major, Journal of Isfahan Medical School (2014)
47. The Effect of Intralesional Meglumine Antimoniate (Glucantime) Versus a Combination of Topical Trichloroacetic Acid 50% and Local Heat Therapy by Non-Ablative Radiofrequency on Cutaneous Leishmaniasis Lesions, Journal of Research in Medical Sciences (2012)
48. Distribution Frequency of Pathogenic Bacteria Isolated From Cutaneus Leishmaniasis Lesions, Korean Journal of Parasitology (2008)
49. Comparison of Protein Profile of Killed and Autoclaved Leishmania Major Using Polyacrylamide Gel Electrophoresis, Journal of Isfahan Medical School (2013)
50. Review of the Prevalence and Causes of Antimony Compounds Resistance in Different Societies: Review Article, Tehran University Medical Journal (2017)