Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Expression of Mtor/70S6k Signaling Pathway in Melanoma Cancer Cells and the Effects of Dacarbazine and Metformin Publisher



Javarsiani MH1 ; Javanmard SH2 ; Sajedianfard J1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
  2. 2. Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Current Cancer Therapy Reviews Published:2022


Abstract

Background: Melanoma, also known as malignant melanoma, is a type of skin cancer that develops from the pigment-producing cells known as melanocytes. Melanomas typically occur in the skin but may rarely occur in the mouth, intestines or, eye. This study aims to examine the expression of the mammalian target of rapamycin (mTOR)/70S6K signaling pathway in melanoma cancer. Methods: The B16F10 cell line treated with dacarbazine IC50 and different concentrations of met-formin (0.5, 2, and 8 mM) for 24 hr and mTOR and 70S6k proteins expression were examined by western blotting. Cell viability was measured by MTT assay. Results: Western blot analysis showed that after different concentrations of metformin and dacar-bazine treatments, the mTOR and 70S6K protein expression significantly (P<0.05) decreased. Conclusion: Metformin-induced repression of mTOR/70S6k axis activity disrupts B16F10 growth. Thus, we believe that combination therapy may be a suitable potential therapeutic target for melanoma cancer. © 2022 Bentham Science Publishers.
Related Docs
View other Related Docs
1. In Vitro Inhibition of Melanoma (B16f10) Viability and Colonization Through Combining Metformin and Dacarbazine, Middle East Journal of Cancer (2020)
2. The Effects of Metformin on the Hippo Pathway in the Proliferation of Melanoma Cancer Cells: A Preclinical Study, Archives of Physiology and Biochemistry (2022)
3. Exploiting New Strategies in Combating Head and Neck Carcinoma: A Comprehensive Review on Phytochemical Approaches Passing Through Pi3k/Akt/Mtor Signaling Pathway, Phytotherapy Research (2024)
4. The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model, Cancer Letters (2018)
5. Administration of Metformin to Increase the Efficacy of Chemotherapy Regimen in Cancers; a New Look to an Old Drug, Immunopathologia Persa (2019)
6. Survivin Promoter-Driven Dff40 Gene Expression Sensitizes Melanoma Cancer Cells to Chemotherapy, International Journal of Toxicology (2021)
7. Exploring Stk3 in Melanoma: A Systematic Review of Signaling Networks and Therapeutic Opportunities, Molecular Biology Reports (2025)
8. Mini Review: The Fda-Approved Prescription Drugs That Target the Mapk Signaling Pathway in Women With Breast Cancer, Breast Disease (2021)
Experts (# of related papers)
View all Related Experts
Shaghayegh Haghjooy Javanmard (10)
Golnaz Vaseghi (5)
Other Related Docs
9. Comparison of Mir-211 Expression in Murine Melanoma Cell Line and Murine Melanoma Tumor by Real-Time Polymerase Chain Reaction, Journal of Isfahan Medical School (2012)
10. Therapeutic Inhibition of Microrna-21 (Mir-21) Using Locked-Nucleic Acid (Lna)-Anti-Mir and Its Effects on the Biological Behaviors of Melanoma Cancer Cells in Preclinical Studies, Cancer Cell International (2020)
11. The Inhibitory Effects of Vanillin on the Growth of Melanoma by Reducing Nuclear Factor-?B Activation, Advanced Biomedical Research (2022)
12. Synergistic Antitumor Effect of Nvp-Bez235 and Cape on Mda-Mb-231 Breast Cancer Cells, Biomedicine and Pharmacotherapy (2017)
13. Atorvastatin Enhances the Antitumor Activity of Tamoxifen in B16f10 Mouse Melanoma Cell Lines, Physiology and Pharmacology (Iran) (2021)
14. Standardized Cannabis Sativa Extract Attenuates Tau and Stathmin Gene Expression in the Melanoma Cell Line, Iranian Journal of Basic Medical Sciences (2017)
15. The Dual Role of Nrf2 in Melanoma: A Systematic Review, BMC Molecular and Cell Biology (2023)
16. Application of Mesenchymal Stem Cells in Melanoma: A Potential Therapeutic Strategy for Delivery of Targeted Agents, Current Medicinal Chemistry (2016)
17. The Role of Dapk2 As a Key Regulatory Element in Various Human Cancers: A Systematic Review, Molecular Biology Reports (2024)
18. Leptin Induces Matrix Metalloproteinase 7 Expression to Promote Ovarian Cancer Cell Invasion by Activating Erk and Jnk Pathways, Journal of Cellular Biochemistry (2018)
19. Activation of Pparγ Inhibits Tlr4 Signal Transduction Pathway in Melanoma Cancer in Vitro, Advanced Pharmaceutical Bulletin (2020)
20. The Effects of Alk5 Inhibition and Simultaneous Inhibition or Activation of Hif-1Α in Melanoma Tumor Growth and Angiogenesis, Tumor Biology (2023)
21. Induction of G2/M Phase Arrest and Apoptosis by a New Tetrahydroingenol Diterpenoid From Euphorbia Erythradenia Bioss. in Melanoma Cancer Cells, Biomedicine and Pharmacotherapy (2017)