Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Sperm Dna Damage and Its Relation With Leukocyte Dna Damage Publisher Pubmed



Babazadeh Z1 ; Razavi S1 ; Tavalaee M2 ; Deemeh MR2, 3 ; Shahidi M4, 5 ; Nasresfahani MH2, 3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy, Isfahan Medical University, Isfahan, Iran
  2. 2. Department of Reproduction and Development, Royan Institute for Animal Biotechnology, ACER, Isfahan, Iran
  3. 3. Isfahan Fertility and Infertility Center, Isfahan, Iran
  4. 4. Department of Medical Physics, Mazandaran Medical University, Mazandaran, Iran
  5. 5. Sari Fertility and Infertility Center, Sari, Iran

Source: Reproductive Toxicology Published:2010


Abstract

DNA fragmentation in human sperm has been related to endogenous and exogenous factors. Exogenous factors can also affect leukocyte DNA integrity. This study evaluated the relation between sperm DNA damage and leukocyte DNA integrity, as a predictor of exogenous factors. DNA damage in the sperm and leukocytes of 41 individuals undergoing ICSI were measured by Comet assay. In addition, sperm chromatin dispersion (SCD) was carried out on semen samples. A positive correlation was observed between the DNA integrity of sperm with leukocytes. When patients were divided into low and high DNA exposure groups, sperm DNA fragmentation was significantly different between the two groups. Cleavage rate and embryo quality showed significant correlation with leukocyte DNA integrity. The results showed that leukocyte DNA integrity could be used to identify individuals at high risk in order to reduce the extent of DNA damage in patients before ICSI in order to improve the subsequent outcome of this procedure. © 2009 Elsevier Inc. All rights reserved.
Related Docs
View other Related Docs
1. Influence of Sperm Chromatin Anomalies on Assisted Reproductive Technology Outcome, Fertility and Sterility (2009)
2. Selection of Sperm Based on Combined Density Gradient and Zeta Method May Improve Icsi Outcome, Human Reproduction (2009)
3. Effect of Sperm Dna Damage and Sperm Protamine Deficiency on Fertilization and Embryo Development Post-Icsi, Reproductive BioMedicine Online (2005)
4. The Role of Sperm Chromatin Anomalies on the Outcome of Assisted Reproductive Techniques, Yakhteh (2006)
5. The Comparison of Efficiency of Density Gradient Centrifugation and Zeta Methods in Separation of Mature Sperm With Normal Chromatin Structure, Yakhteh (2009)
6. Can Zeta Sperm Selection Method, Recover Sperm With Higher Dna Integrity Compare to Density Gradient Centrifugation?, Iranian Journal of Reproductive Medicine (2009)
7. Evaluation of Zeta and Ha-Binding Methods for Selection of Spermatozoa With Normal Morphology, Protamine Content and Dna Integrity, Andrologia (2010)
8. Can Altered Expression of Hspa2 in Varicocele Patients Lead to Abnormal Spermatogenesis?, International Journal of Fertility and Sterility (2010)
Experts (# of related papers)
View all Related Experts
Shahnaz Razavi (26)
Mohammad Mardani (15)
Other Related Docs
9. Evaluation of Sperm Selection Procedure Based on Hyaluronic Acid Binding Ability on Icsi Outcome, Journal of Assisted Reproduction and Genetics (2008)
10. Effect of Saffron on Rat Sperm Chromatin Integrity, Iranian Journal of Reproductive Medicine (2014)
11. The Correlation Between Sperm Dna Fragmentation and the Expression of Long Non-Coding Rna Hotair in Infertile Men, Journal of Isfahan Medical School (2021)
12. Effects of Failed Oocyte Activation and Sperm Protamine Deficiency on Fertilization Post-Icsi, Reproductive BioMedicine Online (2007)
13. Evaluation of Sperm Selection Procedure Based on Its Binding Ability to Hyaluronic Acid on Icsi Outcome, Journal of Isfahan Medical School (2008)
14. Optimizing Acridine Orange Staining for Assessment of Protective Effects of Saffron and Vitamin E on Rat Sperm Dna Structure, Journal of Isfahan Medical School (2014)
15. Comparing the Roles of Sperm Chromatin Integrity and Apoptosis in Intrauterine Insemination Outcomes of Couples With Mild Male and Female Factor Infertility, Journal of Reproduction and Infertility (2014)
16. Effect of Protamine-2 Deficiency on Icsi Outcome, Reproductive BioMedicine Online (2004)
17. Effect of Varicocelectomy on Semen Parameters and Protamine Deficiency 3 and 6 Months Post Surgery, Journal of Isfahan Medical School (2007)
18. Relationship Between Protamine Deficiency With Fertilization Rate and Incidence of Sperm Premature Chromosomal Condensation Post-Icsi, Andrologia (2004)
19. Effects of Keeping Semen Samples in 4-6 °C on Sperm Motility, Viability and Dna Denaturation, Journal of Isfahan Medical School (2016)
20. Protective Effects of Zinc on Rat Sperm Chromatin Integrity Involvement: Dna Methylation, Dna Fragmentation, Ubiquitination and Protamination After Bleomycin Etoposide and Cis-Platin Treatment, Theriogenology (2020)
21. The Epididymal Sperm Viability, Motility and Dna Integrity in Dead Mice Maintained at 4-6°C, Iranian Journal of Reproductive Medicine (2013)
22. Can Sperm Protamine Deficiency Induce Sperm Premature Chromosomal Condensation?, Andrologia (2006)
23. Credibility of Chromomycin A3 Staining in Prediction of Fertility, International Journal of Fertility and Sterility (2009)
24. Correlation Between Sperm Motility and Sperm Chromatin/Dna Damage Before and After Cryopreservation and the Effect of Folic Acid and Nicotinic Acid on Post-Thaw Sperm Quality in Normozoospermic Men, Cell and Tissue Banking (2019)
25. Does Folate Deficiency Alter Sperm Parameters in Infertile Males?, Immunopathologia Persa (2024)
26. Effects of Biotin and Folic Acid on Motility, Viability, Morphology, Chromatin Density and Integrity of Cryopreserved and Thawed Sperm in Normozoospermic Men, Scientific Journal of Kurdistan University of Medical Sciences (2021)
27. Effects of Temperature and Storage Time on the Motility, Viability, Dna Integrity and Apoptosis of Processed Human Spermatozoa, Andrologia (2020)
28. Effect of Human Sperm Chromatin Anomalies on Fertilization Outcome Post-Icsi, Andrologia (2003)
29. Effect of Cholesterol, Iron and Vitamin E on Protamine Deficiency and Dna Fragmentation of Male Rabbit Sperm, Journal of Isfahan Medical School (2014)
30. The Association Between Serum Vitamin D Level and Sperm Parameters: A Pilot Study in a Subset of Iranian Infertile Males, Immunopathologia Persa (2020)
31. The Methylation Status of Gata3 Potentially Predicts the Outcomes of Assisted Reproductive Technologies, Human Fertility (2023)
32. Can the Decreased Expression of Human Leukocyte Antigen Class I and Ii by Spermatozoa Lead to Recurrent Spontaneous Abortion?, Iranian Journal of Pathology (2020)
33. The Association of Food Pattern and Sperm Quality Parameters and Biochemical Markers in Infertile Men: A Review Study, Journal of Health System Research (2020)
34. The Relationship of Oocyte and Sperm Membrane Binding Protein With the Success of Intrauterine Insemination, Journal of Isfahan Medical School (2014)
35. Sperm Mtt Viability Assay: A New Method for Evaluation of Human Sperm Viability, Journal of Assisted Reproduction and Genetics (2002)
36. Failed Fertilization After Icsi and Spermiogenic Defects, Fertility and Sterility (2008)
37. The Effect of Consecutive Ejaculation on the Sperm Parameters in the Oligo-Astheno-Teratozoospermia (Oat) Men, Zygote (2025)
38. Is There a Relationship Between Serum Vitamin D and Semen Parameters? a Cross-Sectional Sample of the Iranian Infertile Men, Basic and Clinical Andrology (2021)
39. Correlation of Obesity and Serum Vitamin D Levels With Sperm Dna Integrity, Sperm Quality, and Sperm Viability in Normozoospermia Men, Advanced Biomedical Research (2022)
40. Varicocelectomy: Semen Parameters and Protamine Deficiency, International Journal of Andrology (2009)
41. Efficiency of Sil-Select and Percoll to Recover Spermatozoa With Normal Chromatin and Morphology and the Effect of These Parameters on Fertilization, Embryo Quality and Cleavage Score, Middle East Fertility Society Journal (2003)
42. Sperm Epigenetics and Male Infertility: Unraveling the Molecular Puzzle, Human Genomics (2024)
43. Resolution of Sperm Quality Impairment Following Sars-Cov-2 Infection: A Prospective Study, Asian Pacific Journal of Reproduction (2024)
44. Relationship Between Sperm Parameters and Intracytoplasmic Sperm Injection Outcome, Middle East Fertility Society Journal (2015)
45. The Expression of Human Leukocyte Antigen by Human Ejaculated Spermatozoa, Molecular Genetics and Genomic Medicine (2019)
46. Effect of Varicocelectomy on Semen Parameters and Sperm Chromatin Status, Yakhteh (2005)
47. Spermatozoa Induce Maternal Mononuclear Cells for Production of Antibody With Cytotoxic Activity on Paternal Blood Mononuclear Cells, Cell Journal (2021)
48. Relationship Between Long Non-Coding Rna Malat1 and Hotair Expression With Sperm Parameters, Dna and Malondialdehyde Levels in Male Infertility, Tissue and Cell (2023)
49. The Association Between Dietary Antioxidants and Semen Parameters: A Cross-Sectional Study Among Iranian Infertile Men, Biological Trace Element Research (2022)
50. Effect of Human Sperm Mtt Viability Test on Outcome of Intracytoplasmic Sperm Injection, Yakhteh (2006)